TFome Information for pUT3407

TFome Name:pUT3407
Genbank Insert ID:KJ726866
Protein Name:ZmbHLH141
Species: Maize
TF Family: bHLH
Template: BT037022
Corresponding TF Gene: GRMZM2G080168
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3277up 3' Primer Name: UT3277dn
5' Primer Sequence: caccATGGCGCACGACGGCTTTC 3' Primer Sequence: GATCGGGCCGCCAATGATC
5' Primer Tm (º C): 65.4 3' Primer Tm (º C): 59
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59.5°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGCGCACGACGGCTTTCTCGGGGGATTCCACGAGCTTTCCTGGCCTCTTGTTCATGCTGCAGCCGCCGGCGCCGCGCC
GTTCGATCCCCTGCTGGTACATGTGCCGCACGCGCTTATGGGCGCCGGTGCGGGGTGGCAACACGAGGCTGCGGTGGGCG
CGATCGTGCCGTCGCAGTCGCAGTCGCAATCGCCACCGCCACCGCCATCGTCGCTCGCGGCGGCCGCCGTAGGAGGAGCA
GATGCCGTTGAGGCCGCCATGATGGAGCAGCTCGCGAGCAGGCTCGGCGTCAGCGTCCCGTCGCCGCCGTCATCGCGGTA
CGTGTCGTGCCACAGTACGCCGGTGGCGCCGTGCGTGCTCGCCGGGGACGCGGAGCGCGCCGAAAGACTGTCGTGCTTCC
CGGCCTCCGGTAGGAAGCTCTCGCGCGTGGCCAGCAGCCAGTCGCTTCTCCGCGAACAGGCTCCGGCGCCCGCGCCCGCG
CCCTCGCCTGGCGCTGCTGCTAAACAGCACGCGGGCGATGGGGCGTGCGATGGCCCCTGCCGCAAGCGGAAGGCGTCGGG
CACGAGCAGCAAGCAGTCCAAGGCCAAGGAAGCGGTCACCACGGCTCCACCCGAGTCCCGGGAGACTGCAGAGACGAGGG
CCAAGAAATGCAAGCTCTCCACGGACGAGGAGCGGAAGCCGGCGGCCGGAGAGGGATGGCGTGGCAGTGGCAAGGGTAAG
GAGCTGGTGGCGGCGGATGCGGAGCCACCCAAGGACTACATCCACGTCCGCGCGCGCCGGGGCCAAGCCACCGACAGCCA
CAGCCTCGCCGAGCGGGTGAGGAGGGAGAAGATCGGCGAGCGCATGAAGCTACTGCAGGACCTCGTGCCGGGCTGCAGCA
AGGTGACCGGGAAGGCCGTGATGCTGGACGAGATCATAAACTACGTGCAGTCGCTGCAACGGCAAGTAGAGTTCTTGTCG
ATGAAGCTGTCGACCGTCAACCCGCGCCTCGAACTCGGCGCGGACGACAGCTTCGTCCCCAGAGACGACGCCAACAAGAT
GTGCGCCGCCGCGACGTCCTCCATCTCCATGGCACAGCAGCCACTGCCACTGCCAGCAGCCTACCACGCGCTCGAGGGCT
CGAGCCCGGCGTTCTGCTACACCACCACGCCCCCAGCTCCCGGCACCGCCGCGCGGCTGGGCGTTGCTGCGTCGGACGCC
AAAGCCTTCGAAATGGCTCCTCCTTCGTCGGCGGCCACCGTGAACCACGCCGGCACCGCGGAGCGGCGGCCACTCGAAGG
ACCTGCTGATGAGAACGCGAGTCCCCAGATGGGGGGGAGGAGCTTGTGGGAAGAGGGCGATGATGATCTGCAGAGCCTAG
TCCTGATGGGATTCCGGGGGCGACACGTGATCATTGGCGGCCCGATC
TFome clones do not contain stop codon at 3'end.
Translation: MAHDGFLGGFHELSWPLVHAAAAGAAPFDPLLVHVPHALMGAGAGWQHEAAVGAIVPSQSQSQSPPPPPSSLAAAAVGGA
DAVEAAMMEQLASRLGVSVPSPPSSRYVSCHSTPVAPCVLAGDAERAERLSCFPASGRKLSRVASSQSLLREQAPAPAPA
PSPGAAAKQHAGDGACDGPCRKRKASGTSSKQSKAKEAVTTAPPESRETAETRAKKCKLSTDEERKPAAGEGWRGSGKGK
ELVAADAEPPKDYIHVRARRGQATDSHSLAERVRREKIGERMKLLQDLVPGCSKVTGKAVMLDEIINYVQSLQRQVEFLS
MKLSTVNPRLELGADDSFVPRDDANKMCAAATSSISMAQQPLPLPAAYHALEGSSPAFCYTTTPPAPGTAARLGVAASDA
KAFEMAPPSSAATVNHAGTAERRPLEGPADENASPQMGGRSLWEEGDDDLQSLVLMGFRGRHVIIGGPI
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map