TFome Information for pUT3408

TFome Name:pUT3408
Genbank Insert ID:KJ726867
Protein Name:ZmbHLH139
Species: Maize
TF Family: bHLH
Template: BT061585
Corresponding TF Gene: GRMZM2G064638
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3280up 3' Primer Name: UT3280dn
5' Primer Sequence: caccATGGGGGACGGCCAGTGC 3' Primer Sequence: CAATAACGTTTGCTGAATTTCCTGCA
5' Primer Tm (º C): 67.5 3' Primer Tm (º C): 56.8
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57.8°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGGGGACGGCCAGTGCGAGCTCGTGGTCGACAACCACTGCCAGCTCCGGCACCGCCTTGACCTGTCTGGAGCCGAGGA
CCTCTTCAGCGTCATCGGGACGTGGGAGGAGCGCACGGACGACGGCGGCGCCGGTGATGGTGGTGGAGGCGGTTCTTCTG
CGCCTGCTATGCGAGCTTATAGGCAGATAAGCTGCAGTGCTGGCGCCGTGGCGGCGACTAGGCTGACTGGGAAGAGCCGG
CGGCGCACGGGCGAGGAGGAGGAGGAGAAAGGCAGTGGCGGCAGCGCTCCCGGGCCCGCGCATAAGAAGCACAATAAGGC
GGGTTCAGCGGTGACAGACGACGACGAGGGGGCGCCGAAGATTTCACATGTGGCGGTGGAGCGCAACCGGAGGAAGCAGA
TGAACGAGCACCTCACCGTGCTGCGCTCGCTCATGCCCTGCTTCTACGTCAAGAGGGGAGACCAAGCGTCCATCATAGGA
GGGGTGGTGGACTACATCAAGGAGCTTCAGCAGGTGCTGCGTTCGCTGGAGGCTAAGAAGCACCGCAAGGCGTATGCGGA
GCAGGTGCTGAGCCCGCGGCCTTCAGCAGGTGGCGTGTCGACGTCGGTATCGGCGGCCAGCCCGCGGCATCTCGCCGTCA
AGTCCGTGGCGCCGCTCAGCCCTCGAATGGCGGTTCCCATAAGCCCCCGGACGCCGACCCCGGGCAGCCCTTACAAGGCA
GCTGCATCCGGCGTGGCTGGATGCTGCAGGCTACCACTACTCCCGCCCTACATGCTGTCGTCGTCGGCGGCGGCGGCTGC
GGCGTCCTACTCGCATGATCAGCAGCAGCACTACTCCACGCAGACGACGACGTACCTGCCGACCCTAGACAGCCTTGTGA
CCGAACTCGCCACGCAGCAGGCAGCTTGCAGGCCAGCGGCGGCGGCGGCCGGGCTCGCCCTCCCCGACGTGAAGGTGGAG
TTTGCAGGGCCCAACCTCGTGCTCAAGACGGTGTCTCACCGCGCGCCGGGGCAGGCGCTCAAGATCATCGCCGCACTCGA
GAGTCTCTCGCTGCAGATCCTTCACGTCAGCGTTAGCGCCGTGGACGACACCATGCTGCACTCCTTCACCATTAAGATTG
GGATCGAGTGCGAGCTTAGCGCGGAGGAGCTTGTGCAGGAAATTCAGCAAACGTTATTG
TFome clones do not contain stop codon at 3'end.
Translation: MGDGQCELVVDNHCQLRHRLDLSGAEDLFSVIGTWEERTDDGGAGDGGGGGSSAPAMRAYRQISCSAGAVAATRLTGKSR
RRTGEEEEEKGSGGSAPGPAHKKHNKAGSAVTDDDEGAPKISHVAVERNRRKQMNEHLTVLRSLMPCFYVKRGDQASIIG
GVVDYIKELQQVLRSLEAKKHRKAYAEQVLSPRPSAGGVSTSVSAASPRHLAVKSVAPLSPRMAVPISPRTPTPGSPYKA
AASGVAGCCRLPLLPPYMLSSSAAAAAASYSHDQQQHYSTQTTTYLPTLDSLVTELATQQAACRPAAAAAGLALPDVKVE
FAGPNLVLKTVSHRAPGQALKIIAALESLSLQILHVSVSAVDDTMLHSFTIKIGIECELSAEELVQEIQQTLL
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map