TFome Information for pUT3410

TFome Name:pUT3410
Genbank Insert ID:KJ726869
Protein Name:ZmbHLH104
Species: Maize
TF Family: bHLH
Template: BT064087
Corresponding TF Gene: GRMZM2G088443
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3322up 3' Primer Name: UT3322dn
5' Primer Sequence: caccATGATGCCTGGTTCAATGGCTGC 3' Primer Sequence: ATGCGACTCAAGAATCATCTGCCG
5' Primer Tm (º C): 64.1 3' Primer Tm (º C): 59.4
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 61.6°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGATGCCTGGTTCAATGGCTGCGCGCCAACCTCTGCAAGGGAAGCAGGAGCTGCAGCCATACGATGGCCGTGACCCTTC
AGCGCTCGGGGCCGGGGGTAGTCCAGTTCTTCTGCCCCGGCAGCAGGAAAGCGCCGCTCCACCGGCCGTGCGGGTAGCGC
CGCCGCCGGACATGTCGTCGTCGTCGGGGTCGGGGAGGAGTGCGACGGAGGCGAGGGCCCTGAAAGTCCACAGCGAGGCC
GAGCGGCGGCGCCGGGAGAGGATCAACGCTCATCTGGCTACGCTCAGGAGGATGGTTCCTGATACCAGGCAGATGGACAA
GGCCACCTTGCTTGCCAGAGTGGTGGAGCAAGTGAAGCTCCTGAAGAGGAAAGCAAGCGAGGCCGCCACCACCACCACCC
AGAGCACGCCCCTGCCCCCGGAGACCGACGAGGTCTCCATCGAGCTCCACACCGGCGACGCCGGCGCCGACAGGTCCGTC
TACATAAGGGCCTCGATAAGCTGCGCCGACCGGCCGGACCTCGTCGCCGGGCTCGCCCAGGCGTTCCATGGCCTGAGGCT
GAAGACGGTGAGGGCCAACATGACCTCGTTAGGAGGAAGGGCGCGGCATGTGTTCGTGCTGTGCATGGAGGAGGGCTGGG
GAAGTGCAGGTGCAGGTGCAGGTGCAAGCCTCAGGTCTCTGAAGGAGGCTGTCAGGCAGGCGTTAGCAAGGGTTGCTTCC
CCAGAAACGGCATACGGGAGTAGTCCCTTTCAGAGCAAGCGGCAGATGATTCTTGAGTCGCAT
TFome clones do not contain stop codon at 3'end.
Translation: MMPGSMAARQPLQGKQELQPYDGRDPSALGAGGSPVLLPRQQESAAPPAVRVAPPPDMSSSSGSGRSATEARALKVHSEA
ERRRRERINAHLATLRRMVPDTRQMDKATLLARVVEQVKLLKRKASEAATTTTQSTPLPPETDEVSIELHTGDAGADRSV
YIRASISCADRPDLVAGLAQAFHGLRLKTVRANMTSLGGRARHVFVLCMEEGWGSAGAGAGASLRSLKEAVRQALARVAS
PETAYGSSPFQSKRQMILESH
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map