TFome Information for pUT3411

TFome Name:pUT3411
Genbank Insert ID:KJ726870
Protein Name:ZmbHLH129
Species: Maize
TF Family: bHLH
Template: BT040920
Corresponding TF Gene: GRMZM5G856837
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3325up 3' Primer Name: UT3325dn
5' Primer Sequence: caccATGATGGACTGCGCTGGAGGC 3' Primer Sequence: AGCATTTGGGGGCCAGAGCT
5' Primer Tm (º C): 65.8 3' Primer Tm (º C): 62.2
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 62.0°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGATGGACTGCGCTGGAGGCCGAGACCCCGTGGAGGACTTCCTCCTCGGCGGCGCCGGCGCCGGCGACGACGGAGATCT
CGTCGCCGTCTTCTGCGACGACGGAGGACTTGGGATTGAGGGTATCCATGGAGATGCTTGTGGATTTGAGCAATCTAATT
TGGGGAAAAGGGGTAGAGATGAACCATGTTCATCTGGCCCAACATCCAAAGCTTGCCGTGAAAAAATGAGGAGGGACAAG
CTGAATGACAGGTTCCTGGAATTAAGTTCAATTATGAATCCTGGTAAGGAAGCAAAGTTAGATAAAGCAAATATATTGAG
TGATGCAGCCCGTATGGTGGCACAACTTAGAGGTGAGGCAGAAAAGCTTAAGGAATCAAATGAGAAGTTGCGGGAGAATA
TCAAGGATCTGAAGGAGGAGAAGAACGAGCTCCGTGAAGAGAAAGTGAGACTAAAGGTAGAAAAGGATAGGCTGGAGCAG
CAAGTCAAAGCTATGAGTGTAGCTCCTACAGGATATGTCCCCCATCTCCCTCATCCAGCTTCATACCATCCTGCTGCGTT
CACTCCATTTGCGCCGCCACAGCAAGCTCCAGCCAACAAAAGTGCTCCAATCCCAGCACCGTTCCCTGGGATGGCGATGT
GGCAGTGGTTGCCTCCGACCATCGTGGACACAACTCAAGATCCAAAGCTCTGGCCCCCAAATGCT
TFome clones do not contain stop codon at 3'end.
Translation: MMDCAGGRDPVEDFLLGGAGAGDDGDLVAVFCDDGGLGIEGIHGDACGFEQSNLGKRGRDEPCSSGPTSKACREKMRRDK
LNDRFLELSSIMNPGKEAKLDKANILSDAARMVAQLRGEAEKLKESNEKLRENIKDLKEEKNELREEKVRLKVEKDRLEQ
QVKAMSVAPTGYVPHLPHPASYHPAAFTPFAPPQQAPANKSAPIPAPFPGMAMWQWLPPTIVDTTQDPKLWPPNA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map