TFome Information for pUT3413

TFome Name:pUT3413
Genbank Insert ID:KJ726872
Protein Name:ZmbHLH7
Species: Maize
TF Family: bHLH
Template: BT034344
Corresponding TF Gene: GRMZM2G042920
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3306up 3' Primer Name: UT3306dn
5' Primer Sequence: caccATGGAGGATGGCAGCAGTGCTC 3' Primer Sequence: CTCCATAAAATTATACGAGGAACTCCTCCC
5' Primer Tm (º C): 65.3 3' Primer Tm (º C): 58
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57.8°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGAGGATGGCAGCAGTGCTCCAAGACGGGCTACTCCCCCCACCCGCAGGAGCAGATCTGCCGACTTCCACAACTTTTC
AGAAAGGAGGAGAAGGGACAGGATCAACGAGAAGCTGAGAGCGTTGCAGGAGCTTCTTCCAAACTGCACCAAGACCGACA
AAGTTTCCATGCTAGATGAAGCGATCGACTACCTAAAATCCCTCCAGCTACAGCTTCAGATGCTGGTCATGGGGAAGGGG
GGAGGAATGGCACCGGTGGTTCCTCCTGAGCTGCAGCAGTACATGCACTACATCACGGCCGACCCCGCCCACCAGATGAT
GCCTCCGCCGCTGCGGCCCTCCGCCGGGCAGCTGCAGCCCGCCGCCGGCCGGCAGTTTCAGATAACCCAAGCAGCAGCAG
CAGCAGCCAACAACGACAACGACCCCCAGCGACGACGACAGTCCAACGTCGAGTCGGACTTCTTGAGCCAGATGCAGAAC
CTGCACCCCTCCGATCAGCCGCCTCATCATCACAACTTCCTCAGGCCGCCGAGATTGCAGCTCTTTACGCCGGAACAGAG
GGGCGGCGGCGGCGGCCTGCCTACCACGAGCCACAACGCCGGCTGGATTTCCGGGAGGAGTTCCTCGTATAATTTTATGG
AG
TFome clones do not contain stop codon at 3'end.
Translation: MEDGSSAPRRATPPTRRSRSADFHNFSERRRRDRINEKLRALQELLPNCTKTDKVSMLDEAIDYLKSLQLQLQMLVMGKG
GGMAPVVPPELQQYMHYITADPAHQMMPPPLRPSAGQLQPAAGRQFQITQAAAAAANNDNDPQRRRQSNVESDFLSQMQN
LHPSDQPPHHHNFLRPPRLQLFTPEQRGGGGGLPTTSHNAGWISGRSSSYNFME
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map