TFome Information for pUT3414

TFome Name:pUT3414
Genbank Insert ID:KJ726873
Protein Name:ZmbHLH161
Species: Maize
TF Family: bHLH
Template: BT038106
Corresponding TF Gene: GRMZM2G045883
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3285up 3' Primer Name: UT3285dn
5' Primer Sequence: caccATGGCGCTCGAAGCTGTGGTGT 3' Primer Sequence: GTGGCCTGGCCCCCCGC
5' Primer Tm (º C): 66.9 3' Primer Tm (º C): 68.9
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 66.0°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGCGCTCGAAGCTGTGGTGTTCCCGAAGGAGCACCCGGGGTGTACGGCGGTCAAGGCGCGCCCGGCGTCGCTGGGCTG
CGGCTTCCACACCGAGGAGCTCGAGGTCGAGGACAAGGGCGGCGTGGTTCTGCAAGGAGAGGCCGCCGACGCGCTGCCGC
CGCCCGGTGGTGCCGCCACCACGTGGGACTGGGACGCGGTGCTCTGCCCGTGCTCCACTGCACCAGCTGCAGGCGCCGTG
GAGGAGTGCTGGGACGTGCAGCAGCAGCAGCATCAGCTCCTGTCCGTGTCCCCGCCGCCCTCGTCCGCCGTCGCAGCGTC
GTCGTCTGGGCGCCGCAAGGCTGCCGCGTCGGAGGCGGCGCGGCGACGGCGGCGACGCCCCAAGGCGGTGAGGAACACGG
ACGAGATGGAGAGCCAGCGCCGCAACCACATTGCCGTGGAGCGCAACCGGCGGCGGCAGATGAACGAGTACCTCTCCGTG
CTCCGCTCCGCCATGCCGCCTTCCTACACGCAGCGGGGTGACCAGGCGTCGATCGTGGCTGGTGCCATCAACTTCGTCAA
GGAGCTGGAGCAGCTGCTGCAGTCGCTGGAGGCGCAGAAGCGGTGCACCGAGCCACCGGCGCCGGCGCCGTTCGCCGGCT
TCTTCATCTTCCCTCAGTACTCCACCACCGCCGCGACGGGTGGTGCGGTTGGCTCTAGTAGCGACAGCGCTGGCTCCGGC
GGCGATCAGAGTGGCGGCGGCGGCGGCTGCGCGGGAGCGCGGCGAGGCGTGGCCGACATCGAGGTGTCCCTGGCGGAGAG
CCACGCCAACGTGAAGGTGCTCGCGGCGCGGCGGCCGCGGCAGCTGCTGAGGATGGTGGTGGCGCTGCAGTGCCTCGGCC
TCACCGTGCTCCACCTCAACGTGACCACCACCGCCGACCACCTGGCCTTGTACTCCCTCAGCCTCAAGATGGAGGACGAG
TGCCGGCTGTCGCCGTCGGTCGACGACATCGCCGCCGCGGTGAACGAGATACTCGCCGATATCTCCGACGAGTGCGCCCG
CGGGGGGCCAGGCCAC
TFome clones do not contain stop codon at 3'end.
Translation: MALEAVVFPKEHPGCTAVKARPASLGCGFHTEELEVEDKGGVVLQGEAADALPPPGGAATTWDWDAVLCPCSTAPAAGAV
EECWDVQQQQHQLLSVSPPPSSAVAASSSGRRKAAASEAARRRRRRPKAVRNTDEMESQRRNHIAVERNRRRQMNEYLSV
LRSAMPPSYTQRGDQASIVAGAINFVKELEQLLQSLEAQKRCTEPPAPAPFAGFFIFPQYSTTAATGGAVGSSSDSAGSG
GDQSGGGGGCAGARRGVADIEVSLAESHANVKVLAARRPRQLLRMVVALQCLGLTVLHLNVTTTADHLALYSLSLKMEDE
CRLSPSVDDIAAAVNEILADISDECARGGPGH
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map