TFome Information for pUT3421

TFome Name:pUT3421
Genbank Insert ID:KJ726880
Protein Name:ZmABI14
Species: Maize
TF Family: ABI3-VP1
Template: BT037444
Corresponding TF Gene: GRMZM2G106673
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2843up 3' Primer Name: UT-2843dn
5' Primer Sequence: caccATGATCTCACATACTGCAGCAATAACAA 3' Primer Sequence: GTCCTCGCTTTCGTAGTAGGAACTTG
5' Primer Tm (º C): 57.3 3' Primer Tm (º C): 57.9
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; Step 4, 4°C hold. 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, template 40pg/ul final concentration.
Nucleotide Sequence: ATGATCTCACATACTGCAGCAATAACAATATACACAAAGACGGGAGAGAGAAGAGAGATGGCGGAAGCGACGCCGTACGA
GGAGCAGCGGCGGAGGCAGGTGGAGGCGAACAAACGGAAGCTGGAGGAGCTGCAGCTCCACCACCTCTCTGCTGCCATCA
GAGAGGCCGCCGCCGCCGCCAAGCCCTCGCTGGTCAAGAAGCGGAAGGCGCGGGTGCCTCTCGACGTCGCCGACGCCGTG
ATGGAGCCAGTACGGCGGTCCGGCCGGCTCGCCAACCTCCCGGATAAGCCCGTGTACCGCGAGAAGGTTCCCGATTTTGG
GATGAAGATTAGGAGGACGTACGGATCGGTGAGAAGAGATCTGACCAACCGGGTGTACGCGACCGACGATGCGAGGAGCT
ACGCCATCTCTAAGGCGGAGGATCTGGAGCAAGAGCTGGACTCCAGCTTCCCCATCTTCATCAAGCCCATGACCCAGTCC
CATGTCACCGGGGGCTTCTGGCTGGGCCTTCCGATTCATTTCTGCCGGAAGTATCTGCCCAAGCGTGACGAGATGATCAC
CCTGGTGGATGAGGATGATGACGAGTCCGACACGCTGTACCTCGCAATGAAGCGGGGGCTCAGTGCTGGATGGAGAGGGT
TTGCCGTCCAGCAGAAGCTGGTGGATGGAGATTGCCTGGTCTTCCAGCTGATTGAGCAAACAAAGTTCAAGGTCTACATA
ACTAGAGCAAGTTCCTACTACGAAAGCGAGGAC
TFome clones do not contain stop codon at 3'end.
Translation: MISHTAAITIYTKTGERREMAEATPYEEQRRRQVEANKRKLEELQLHHLSAAIREAAAAAKPSLVKKRKARVPLDVADAV
MEPVRRSGRLANLPDKPVYREKVPDFGMKIRRTYGSVRRDLTNRVYATDDARSYAISKAEDLEQELDSSFPIFIKPMTQS
HVTGGFWLGLPIHFCRKYLPKRDEMITLVDEDDDESDTLYLAMKRGLSAGWRGFAVQQKLVDGDCLVFQLIEQTKFKVYI
TRASSYYESED
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map