TFome Information for pUT3423

TFome Name:pUT3423
Genbank Insert ID:KJ726882
Protein Name:ZmbHLH106
Species: Maize
TF Family: bHLH
Template: BT053989
Corresponding TF Gene: GRMZM2G089501
Transcript: T02
Vector: pENTR/SD/D-TOPO
Note: c.A894G p.(=) c.G1200A p.P(=) Present in template FlcDNA.

5' Primer Name: UT3318up 3' Primer Name: UT3318dn
5' Primer Sequence: caccATGGGCATCCAAGGGAACAAGG 3' Primer Sequence: GCTGTTGCTCCGTTTATGCCG
5' Primer Tm (º C): 63.7 3' Primer Tm (º C): 59.3
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59.5°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGGCATCCAAGGGAACAAGGCGGCGACGCACGAGCACGACTTCCTCTCGCTGTACGCCGCCGCCGCCGCCAAGGACGC
TCCTCTCCTGCTACACGACTCCAAGGCGCCGCCTCCTTCTCAAGGTAATTTCCTCTTGAAGACGCACGACTTCCTGCAGC
CGTTGGATCAGAAGCCGGGAGCGCCGCCGGAGCCGTCGCCGCTGCCCGCTTCCGCCGCGGAGAGCAGGCACCAGCCGCAG
CAGCAGGTCGTCGTCGCGAACCAGCACGCGCTGCCCCTGCCCGGCGGCGTCGGCACCTTCAGCATCAGCCCCGCGCCCGT
GTCCGTCGCGCTGCCGGCGGCCGTCGTCAAGTCGGAGCCGCCGTTCGTGCTCTGGGGCCAGCCTGCCGCGACGCTCCATC
CGGGCGCGCGAGCAGGGCACCAGCAGCAGTGGGCGCTCCCCTTCGCCGGCGCCGGGCAGGTCAGGCTGCCGCCGCAGCAG
GCGCCTCCGGATAGAAAGGGCCGGGGCGGCGCCGGTGGCGGCGTCATGGAGTCTGGCTCCAGATCCAGCGGCGGGGCGGG
GTTCGACGACGACGACGGCCTCACGACGCGCCGCGAGGTCTCCTCTTCGCTCCAAGATTTGACAGTAAGGGTGGACCGGA
AGGGTGGGAGCTGCAGCGATGGTGGTACGGATCAGCGGCCAAACACACCGCGGTCAAAACACTCGGCCACAGAGCAGCGC
AGACGCAGCAAGATCAATGATAGGTTTCAGATACTTAGGGAGCTGTTGCCACACAGTGATCAAAAGAGAGACAAAGCAAC
ATTTCTCTTGGAGGTTATTGAATATATACGGTTTTTGCAAGAGAAGGTGCAAAAATATGAGGCAACATTTCCAGAATGGA
ACCAAGAAAATGCGAAGATGCTTCCATGGTCAAATATGTATTTTCGATCATTCTGGAAAAATGCACAGAGTAAAGGCCAA
ATCTCAGGAGATTCCCCGCCTGACCCTTCACATTTCATGAGAAATGGATCCTCCCCTGGATCTAATTTCACTGGGAAACT
TGATGATAATCACAACATAGTGACATCTGCAGCTGCATCAGGGGCACAGGATCAGGTAGAAACCGACCACATGGCTAGCG
GATGCTACAGATCAGCAGAAACTACGGCGAATTTTACAAATAATGCTATGTCTCAGTCCCAACCTCAATGGACTGGTCCA
TCCCCTGTGGATGACTCTGCAGTGAACAGTGAGACGCTTAACAACCAGCAGTTGGTGATTGATGAAGGAACAATCAGAGT
GTCCAGTAACTATTCCCAAGAGTTGCTTAACTCGTTGACTCATGCCCTTCAAAGCTCAGGTGTAGATTTGTCCCAAGCCA
ACATCTCTGTGCAGATCAACATGGGAAAGCGTGCCGCCAAGAGACCCGCTGCTGGTGTATCCAGCAACTCCAAGGAACCA
GCTGATCCGGCATCTAGCAACGAATTAGGGCATCAGTTGACGCTGTCGGGTGCCGGTGCCGACTGCCTCTCTCACGCAAC
AAAGCGGCATAAACGGAGCAACAGC
TFome clones do not contain stop codon at 3'end.
Translation: MGIQGNKAATHEHDFLSLYAAAAAKDAPLLLHDSKAPPPSQGNFLLKTHDFLQPLDQKPGAPPEPSPLPASAAESRHQPQ
QQVVVANQHALPLPGGVGTFSISPAPVSVALPAAVVKSEPPFVLWGQPAATLHPGARAGHQQQWALPFAGAGQVRLPPQQ
APPDRKGRGGAGGGVMESGSRSSGGAGFDDDDGLTTRREVSSSLQDLTVRVDRKGGSCSDGGTDQRPNTPRSKHSATEQR
RRSKINDRFQILRELLPHSDQKRDKATFLLEVIEYIRFLQEKVQKYEATFPEWNQENAKMLPWSNMYFRSFWKNAQSKGQ
ISGDSPPDPSHFMRNGSSPGSNFTGKLDDNHNIVTSAAASGAQDQVETDHMASGCYRSAETTANFTNNAMSQSQPQWTGP
SPVDDSAVNSETLNNQQLVIDEGTIRVSSNYSQELLNSLTHALQSSGVDLSQANISVQINMGKRAAKRPAAGVSSNSKEP
ADPASSNELGHQLTLSGAGADCLSHATKRHKRSNS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map