TFome Information for pUT3428

TFome Name:pUT3428
Genbank Insert ID:KJ726887
Protein Name:ZmbHLH20
Species: Maize
TF Family: bHLH
Template: BT037825
Corresponding TF Gene: GRMZM2G414252
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3283up 3' Primer Name: UT3283dn
5' Primer Sequence: caccATGGAGTTCGACCTGCTGAATTATTACAG 3' Primer Sequence: CAGCATGTCGGCGTGGGG
5' Primer Tm (º C): 61.5 3' Primer Tm (º C): 62.2
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59.5°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGAGTTCGACCTGCTGAATTATTACAGCCCGGAAGAAGCGCAGCTTGAGCTGATGAACACGATGCTCCAGCTGGAGCA
GCTCACCGCGCTCGACGGCCATCGTCAGTCGGCGATGGCACCTGCCTCGCCGCCGATATCCCCGATGCAGACCCACCATG
CAGGTCCCCACAGCTTCTCGCCTCTGCCACACACGTCGACGACCACGACCACGACCGCCACCGCCACCGGGTACCCAAAG
AAGCAGCACGCACCGCCACGGGCCGTGTACGGCGCCGCCACTTCCAGCGGCCTCGAGCACCACCACCTCCAGCTCCAGGA
CGGGGGCGCGCTGCAGGCCGGGCCGTCGTCGGCGGCGGCGGCGTCGGCGGACGCGATGCGGGAGGCGATCTTCCGCATCG
CCGCGCTGCAGCCGGTGGAGGATCAGACGACTACGGGCCCGGAGCCGGAGGCGGCGGTGCGGCGGGCCCCGAAGCGGCGC
AACGTGCGCGTGTCCAAGGACCCGCAGAGCGTGGCGGCGCGGCTGCGGCGGGAGCGCATCAGCGAGCGCATCCGCGCCCT
GCAGCGCCTCGTCCCGGGCGGCACCAAGATGGACACGGCCTCCATGCTGGACGAGGCCATCCAGTACGTCAAGTTCCTCA
AGTCCCAGCTGCAGTCCCTGCAGCGCGCCGCCGCCTCCCGCCACGCGCCGTGGCAGTACGCGCTCCTTCCCCACGCCGAC
ATGCTG
TFome clones do not contain stop codon at 3'end.
Translation: MEFDLLNYYSPEEAQLELMNTMLQLEQLTALDGHRQSAMAPASPPISPMQTHHAGPHSFSPLPHTSTTTTTTATATGYPK
KQHAPPRAVYGAATSSGLEHHHLQLQDGGALQAGPSSAAAASADAMREAIFRIAALQPVEDQTTTGPEPEAAVRRAPKRR
NVRVSKDPQSVAARLRRERISERIRALQRLVPGGTKMDTASMLDEAIQYVKFLKSQLQSLQRAAASRHAPWQYALLPHAD
ML
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map