TFome Information for pUT3430

TFome Name:pUT3430
Genbank Insert ID:KJ726888
Protein Name:ZmbHLH67
Species: Maize
TF Family: bHLH
Template: BT062649
Corresponding TF Gene: GRMZM2G397518
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3292up 3' Primer Name: UT3292dn
5' Primer Sequence: caccATGGATCCATATCACTACCAAACCATGTATG 3' Primer Sequence: GTAAGAGCCGGGCAGACCGG
5' Primer Tm (º C): 61.1 3' Primer Tm (º C): 62.8
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59.5°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGATCCATATCACTACCAAACCATGTATGACCCACGCGGCTTCCCCATCATCCACCCGCAGCCTTACCTCCAGCACCC
GGTGGCCGGCGCCCTCGGTGACAGCAGGGTGCGCGGCGGCGGCAGTGGCGCGCGGCGGCGTCCTGGCGCCAAGCTCTCCA
CGGACCCGCAGAGCGTTGCGGCGCGCGAGCGGCGGCACCGCATCAGCGACCGCTTCCGCGTGCTCCGCAGCCTCGTCCCC
GGCGGCAGCAAGATGGACACTGTGTCCATGCTCGAGCAGGCCATCCACTACGTCAAGTTCCTCAAGACGCAGATCAGCCT
GCATCAGGCCGCGCTGATGCAGCACGAGGAAGGATGCCATGCTGAGCTCGCCGCCTATTCCGCGGTGGCGGTGGTTGGTG
ACAACGAGGTGACACTCGCGTCCCATGGTCGTACCGGCGCATGCGACGAGATGATGCAGCTCCAGGTGGCGGCGGAGGAA
GCTTTGAGTTATGGTGTTGATGCCCATCAGCCGTACGGGCTCGATCCCAGGCAGCTGAGTGGTGGGCACGAGCTGCCACC
GCTGCCTGCTTCTTGCATCTTCCTCGAGGAGCCTGCAGACGCATGCTACTCTGTGTGTGACCTCGACGACGGGGACACCG
GTCTGCCCGGCTCTTAC
TFome clones do not contain stop codon at 3'end.
Translation: MDPYHYQTMYDPRGFPIIHPQPYLQHPVAGALGDSRVRGGGSGARRRPGAKLSTDPQSVAARERRHRISDRFRVLRSLVP
GGSKMDTVSMLEQAIHYVKFLKTQISLHQAALMQHEEGCHAELAAYSAVAVVGDNEVTLASHGRTGACDEMMQLQVAAEE
ALSYGVDAHQPYGLDPRQLSGGHELPPLPASCIFLEEPADACYSVCDLDDGDTGLPGSY
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map