TFome Information for pUT3432

TFome Name:pUT3432
Genbank Insert ID:KJ726889
Protein Name:ZmbHLH53
Species: Maize
TF Family: bHLH
Template: BT038655
Corresponding TF Gene: GRMZM2G111146
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3289up 3' Primer Name: UT3289dn
5' Primer Sequence: caccATGTCGGCGCTAGTGGACGCG 3' Primer Sequence: TTGCGGTTGCGGCCCCG
5' Primer Tm (º C): 67.4 3' Primer Tm (º C): 65.6
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 63.6°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGTCGGCGCTAGTGGACGCGCTGTGCGCGCCGCCCAGCGACACCGCGCTCGTCTACGACACCTTCAACGCGGCCAACTT
CCTCTTCGACGCCGCCGCGTTCTGTGACGGCGCCGGCATTGTCGCCGCCCCGGCGTCCGCGCACCAGGCGCCCGTGGAGG
CGCAGGCAGCGACGACGTCGGCGGCGCCGACGACGACGGCTAGGGCCAGGAAGCGGCGGCGGCGGCCGAGGAGCTGCAAG
TCCAGGGAGGAGACCGAGACCCAGCGGATGACGCACATCGCCGTCGAGCGCAACCGCCGCCGCCAGATGAACGAGTACCT
CGCCGCGCTCCGCTCCATCATGCCGGAGGCCTACGTCCAGAGGGGCGACCAAGCTTCCATCGTCGGAGGGGCAATCGAGT
TCGTGAAGGAGCTGGAGCAGCAGGTCCAGTGCCTCGAGGCGCAGAAGCGGAAGCTGCTGGTTCACCAGCGCGTGGCGGCG
GCGAAGCCTGCAGACGCGACGCCAATGCGCGCCAGCAGCAGCGGCGCCAGGGCCTGCTGCGCGGATTCGGCGGCGGCGGC
GGCGACGACGAGCAACTGCAGCAGCGGCTTGACGACGGAGGACGCGGCGGCGGACCACGCGCTGCCGCCGCCCCCGTTCG
CGCAGTTCTTCACGTACCCGCAGTACGTGTGGTGCCACTCGGCGGCGCGCGACCCCGCGGAGGAGGAGGACGGGCGGCGG
TCAGGGGTGGCCGACGTCGAGGTGACCCTGGTGGAGACGCACGCCAGCGTCCGAGTGATGACGGCGCGGCGTCCCGGGCA
GCTGCTCAGCCTGGTCACCGGGCTGCAGGCGCTCCGCCTCGCCGTGCTCCACCTCAGCGTCGTCACCGCGCTGGACGCCC
TGGTCCTCTACACCACCAGTGTCAAGGTGGAGGAAGGGTGCGGCCTGGCGACGGCGGACGACATCGCCGCCGCTGTGCAC
CACGTGCTCTGCATCATCGACGCCGAGGCCACCGAGCAGCAGCTGCTCGCGGCAGCGGGGCCGCAACCGCAA
TFome clones do not contain stop codon at 3'end.
Translation: MSALVDALCAPPSDTALVYDTFNAANFLFDAAAFCDGAGIVAAPASAHQAPVEAQAATTSAAPTTTARARKRRRRPRSCK
SREETETQRMTHIAVERNRRRQMNEYLAALRSIMPEAYVQRGDQASIVGGAIEFVKELEQQVQCLEAQKRKLLVHQRVAA
AKPADATPMRASSSGARACCADSAAAAATTSNCSSGLTTEDAAADHALPPPPFAQFFTYPQYVWCHSAARDPAEEEDGRR
SGVADVEVTLVETHASVRVMTARRPGQLLSLVTGLQALRLAVLHLSVVTALDALVLYTTSVKVEEGCGLATADDIAAAVH
HVLCIIDAEATEQQLLAAAGPQPQ
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map