TFome Information for pUT3433

TFome Name:pUT3433
Genbank Insert ID:KJ726890
Protein Name:ZmbHLH118
Species: Maize
TF Family: bHLH
Template: BT040271
Corresponding TF Gene: GRMZM2G061906
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3316up 3' Primer Name: UT3316dn
5' Primer Sequence: caccATGAGCTGCGCGGGGCAG 3' Primer Sequence: GGCGACCGGCGGCCAGT
5' Primer Tm (º C): 67.8 3' Primer Tm (º C): 67.6
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 64.9°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGAGCTGCGCGGGGCAGGCCGGCGGGTGGCTGCTCGACTACGCGCTGGTGGAGGAGGAGATCCAGGGCTCCGAGTTCAT
CTACATGGTCGACGACCCGGCCGTCTCCAGCGTGATACTGGGGTTTGATGCTCCCAGGAAGGAGGACGGCGTCCAGGACA
ACCCCGGCGCCAAGAAGAGATCCCGGCCGGAGTCCAGCGCGCCACCCGGCACCAAGGCTTGCCGTGAGAAGCTCCGGCGA
GACAGGCTCAACGAAAGGTTCAACGAGCTCTGTGCCATCTTGGAACCCGGCAAGCCACCCAAGGCCGACAAAGTCGCTAT
CCTAAGCGACGCTGCCCGTCTCCTGAACCAGCTGCAGGGCGAGGCCCAGAAGCTTAAGCAATCGAACGAATCGCTCCAGG
AGTCCATCAAGAGTCTTAAGGCTGAGAAGTCCGAGCTGCGAGACGAGAAGACGAGGCTCAAGGCTGAGAAGGAGAGGTTG
GAGCAGATGCTCAAGGGCGTCGTCAGCCACGCCGCGGTGGCTGCTCCGGGGCCCTTCGTCCCGCACCCTGCTGCGGTCGC
TCCATCTTTCCACCACCACCCGGCGGCGTTTGCTCAGGCTGGAAAGTTCGTCCCGTACCCCAGCTACCCGCCGCCGGCTG
CGTTCTGGCAGTGGATACCGCCGACGTCCCTCGACACGACCAAGGACCCCGCACACTGGCCGCCGGTCGCC
TFome clones do not contain stop codon at 3'end.
Translation: MSCAGQAGGWLLDYALVEEEIQGSEFIYMVDDPAVSSVILGFDAPRKEDGVQDNPGAKKRSRPESSAPPGTKACREKLRR
DRLNERFNELCAILEPGKPPKADKVAILSDAARLLNQLQGEAQKLKQSNESLQESIKSLKAEKSELRDEKTRLKAEKERL
EQMLKGVVSHAAVAAPGPFVPHPAAVAPSFHHHPAAFAQAGKFVPYPSYPPPAAFWQWIPPTSLDTTKDPAHWPPVA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map