TFome Information for pUT3437

TFome Name:pUT3437
Genbank Insert ID:KJ726894
Protein Name:ZmbHLH151
Species: Maize
TF Family: bHLH
Template: DR796485
Corresponding TF Gene: GRMZM2G137541
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3250up 3' Primer Name: UT3250dn
5' Primer Sequence: caccATGGACATGAACGAGAGCGGCG 3' Primer Sequence: GATTTGAAACACGTCCTGTTGCGAC
5' Primer Tm (º C): 65.2 3' Primer Tm (º C): 58.8
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 61.6°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGACATGAACGAGAGCGGCGAGACAGGGATGGAGCAGGGCAACGCGTCCTCCGGCGCCCCGGTGGATTGGCAGACTCA
ATTCAACGCCGCCGCGTTCTCGTGCGCGCCGCAGCAGGTTCCCATGATGGACTCCGCCTTCGCGTCCGCCGGCCTGTGGG
CGTCCACCTCCCAAGCCATGGCGCTCTCCGACGTCGTCGGCGCCATGTCGGCCGCGCGGGGAGGCGGGTTCTTGGCGCCG
GTGCCCGGTTTCCTCCCGCAGGGCCTCGGCCATTTCGCCGTCGACTCCGGCTTCATCGAGCGCGCCGCGCGGACGTCCTG
CTTCAGCGGCGGCGGCGGCGGGGTGATGGGCGCTAGCGCAGCTGCCTTCGGTGCGGCTGGTCAGACCGTGAACAACGCGT
TCAGTGGTTCGTCAGAGGCGCTGCTGCTGGATCACCAGAAGAAGGACGTCGTCGGCGAGAAGGGCGAGCCGGAGCTCGGC
CGCGACGACGGGGTGCTGAGCCCGGAGGCTGCTGGTGGTGGGGACTGCTCGTCCAAGGGGACGTCGGACTCCAAGAAGAG
GAGAAGGCCCAACGAGGTGGTGGGAAGCGATCAGGTCCAGTCGGCCAACCTGCCCAGCGCCGACTCGGCGAACGAGAGCG
TGCACAGCATGGACAAAGGCGAGGAGAGCAGCCCGGCAACAACCACCGCCGGCGCCGGGCCTGGCAAGTCGAGAGGGAAG
GGAGAGAAAGAGGTCCCCGAGTCTCAGAAGGAGGACTACATCCATATCCGAGCCCGCCGCGGACAGGCAACGAACAGCCA
CAGCCTAGCAGAACGGTTGAGGAGGGAGAAGATTAGCGAGAGGATGAAGCTTCTGCAGGACCTTGTTCCTGGTTGCAGCA
AAGTCACTGGCAAGGCGGTGATGCTTGACGAGATCATCAACTACGTTCAGTCCCTGCAAAGACAAGTTGAGTTCTTGTCA
ATGAAGCTCGCCACGGTGAACCCAAGGCTTGACCTCAACATAGAAGGGCTTCTATCGAAAGACCTTCTCCGCTTCCCTGG
TGCTCCTTCGTCCTCCCTCGGGTTTCCCCCAGAAATGATGCACCCGCAGCTACTACAGCTGTCGCAGCCAGGCCGCCTGG
TCCAGGGAGCCGCCGCCGCCGCCGCCGGCATGGCCAATCCGGACGTGTTCAGGAGAATCATGCAGGCGCAGCTGAGCGCG
AAAGACGACAGCGGCGGCGCCTCTCAGATGCTGCCCCACGGGTCGTTCGGTGGCGTTGCGCGGGCGGCGTACCCGTCGTC
GTCGTCGGCCCTGGCGCTGGGGTCCCACGACTACTTGAGGAGCATGAGCAGGGCGTCGCAACAGGACGTGTTTCAAATC

TFome clones do not contain stop codon at 3'end.
Translation: MDMNESGETGMEQGNASSGAPVDWQTQFNAAAFSCAPQQVPMMDSAFASAGLWASTSQAMALSDVVGAMSAARGGGFLAP
VPGFLPQGLGHFAVDSGFIERAARTSCFSGGGGGVMGASAAAFGAAGQTVNNAFSGSSEALLLDHQKKDVVGEKGEPELG
RDDGVLSPEAAGGGDCSSKGTSDSKKRRRPNEVVGSDQVQSANLPSADSANESVHSMDKGEESSPATTTAGAGPGKSRGK
GEKEVPESQKEDYIHIRARRGQATNSHSLAERLRREKISERMKLLQDLVPGCSKVTGKAVMLDEIINYVQSLQRQVEFLS
MKLATVNPRLDLNIEGLLSKDLLRFPGAPSSSLGFPPEMMHPQLLQLSQPGRLVQGAAAAAAGMANPDVFRRIMQAQLSA
KDDSGGASQMLPHGSFGGVARAAYPSSSSALALGSHDYLRSMSRASQQDVFQI
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map