TFome Information for pUT3438

TFome Name:pUT3438
Genbank Insert ID:KJ726895
Protein Name:ZmbHLH81
Species: Maize
TF Family: bHLH
Template: BT064492
Corresponding TF Gene: GRMZM5G828396
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3334up 3' Primer Name: UT3334dn
5' Primer Sequence: caccATGAAAGAGGGCAGACCCGGC 3' Primer Sequence: GAAGAACTCCATCTTCATGTGGCCCT
5' Primer Tm (º C): 65.7 3' Primer Tm (º C): 60.6
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 60.9°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGAAAGAGGGCAGACCCGGCGCCGGCGACTACATTGCCAGCCTGCTAAGCTCGTCGCCGAGGCTCGACTTCGGCGGGGA
GGAGGAGGAGGACTGCCTGGACAGGTTCTGCGGCGACCCGGGGTTCGCGGCTCGCGCGGCGCGGCTGTCCAGCTTCAGCG
GGCAGCGCTTCGCCGTCACCGCGGGCCTCTTCGGGCTGCCCCCGCCGCTGCCCGCCGCGAGCGGCGGCGGCGAGTTCGCC
GGGTCCCGGGAGGCGTCGTCGGTGTCGGACCCGGCGTCGGCGATGAAGGACGCCAATGCCAAGAAGCGGAAGGCGCCCGC
GGCGGCGGCGGCCAAGGGCAAAGGCAGGGAGCCATCTGCGCAGGCACAGGCAGGGGAGCCGAAGGGTCCGGACGCCAAGA
GGTGCTGCAAGGCGGAGGGAGGCGAGGGGGAGGAGGGGAGCCCCGTGAAGCTGCCCAAGCCGGAGCAGGCCGGCAGCGAC
AGCTCCGTCGAGGACGGCGGCGCGCAGAACCAGAAGCCGCCGCCGCCGGTGAAGGGAAAGAATGCCAAGCCGGTGGAGCC
TCCCAGGGACTACGTCCATGTCCGGGCCAGGAGAGGGCAGGCTACCGACAGCCACAGCCTCGCAGAGAGGGTGAGAAGGG
AGAGGATCAGCCAGAGGATGAAGGTCCTTCAGGACCTGGTGCCAGGATGCAACAAGGTGATCGGCAAGGCGCTGATGCTC
GACGAGATCATAAACTACGTGCAGTCGCTGCAACGGCAGGTCGAGTTCCTCTCCATGAAGCTCGCAACCGTCAACCCACT
GGACCTGAGCAACCTGCCCACGCTCCTACAAAAAGATGCCTGCGGCGCTTCGGCGAGCTCGGTCTTCTCGCTGGAGAGCT
GCAGCCCGGGGTTCCCGTTCGGCGGGCAAGGGGACGTGTTCCAGTCCTTCGTCCCGAACGGCCTGGAGAACCCGTGCGGC
GGCCTGAACCCGCTGGACCTGGCTCTGTCCCAGGCTACCGGCGGGCAGTTCGGTTTCCAGGACGGAACGGCCGGCACAAA
CCTGCAGCAAAGGAACTACTGGGAGGAGGAGGAGGAGGACCTGCAGAGCGTGTTCCACATCGACGACAACGGGCAGAGCC
AGGAGCACGGGGCCTCAGCTTCAGCACAAAGCTTCCATGGTCAGCTTCAACCACAAGAGGGCCACATGAAGATGGAGTTC
TTC
TFome clones do not contain stop codon at 3'end.
Translation: MKEGRPGAGDYIASLLSSSPRLDFGGEEEEDCLDRFCGDPGFAARAARLSSFSGQRFAVTAGLFGLPPPLPAASGGGEFA
GSREASSVSDPASAMKDANAKKRKAPAAAAAKGKGREPSAQAQAGEPKGPDAKRCCKAEGGEGEEGSPVKLPKPEQAGSD
SSVEDGGAQNQKPPPPVKGKNAKPVEPPRDYVHVRARRGQATDSHSLAERVRRERISQRMKVLQDLVPGCNKVIGKALML
DEIINYVQSLQRQVEFLSMKLATVNPLDLSNLPTLLQKDACGASASSVFSLESCSPGFPFGGQGDVFQSFVPNGLENPCG
GLNPLDLALSQATGGQFGFQDGTAGTNLQQRNYWEEEEEDLQSVFHIDDNGQSQEHGASASAQSFHGQLQPQEGHMKMEF
F
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map