TFome Information for pUT3457

TFome Name:pUT3457
Genbank Insert ID:KJ726912
Protein Name:ZmbZIP91
Species: Maize
TF Family: bZIP
Template: BT061202.1
Corresponding TF Gene: GRMZM2G060109
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B483_F 3' Primer Name: B483_R
5' Primer Sequence: caccATGGCGGAACCGGCCCT 3' Primer Sequence: AAGGTGAGGGCTATGGCTTTTGAACGG
5' Primer Tm (º C): 72.4 3' Primer Tm (º C): 69.4
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGCGGAACCGGCCCTCCTCCCGCCGGACCCCTTCGCGGACCTCCCCTTCCCCGAATTTCAGGCGCCCATCGACGGCGA
CAGCTTCGCGCTTGAGGACTTCGATCTGGAGGATCTGGACCTGGACGTGGACTTCGACCTCGACCTCTTCGGCTCGGACG
GGCAACCCTCGCAACCGCCCCCGCTCGCGACCTCGTCCTCCTCGGCCGGGTCTCCGGTTGGAGGCTCGTCCTCTTCCGGT
GCCGTCGGGGACGGCGGAGGGCTGAGGAATGAGGAGTCTTCGGAGTCGTCTTCGAGGAGCGCGAGCGGCACGGATGGCAG
CGGCAAGGGGAAGGGGGAGGAGGACGAAGCGAAGCGGCGCGCGCGGCAGGTACGCAACCGCGAGAGCGCGCACCTGTCGC
GGCAGAGGAAGAAGCAGTACGTGGAGGAGTTAGAGGGTAAGGTGAAGGCCATGCAGGCCACCATCGCCGACCTCTCCGCC
AGGATCTCCTGCGTCACCGCCGAGAACGCCGCTCTCAAACAGCAGCTGGGTGGCGCTGCTGGGGCCGCGCCGCCGCCGAT
GCCGATGTACCCCACGATGTATTCTTTGCCGATGCCGTGGATGCACCCATCGTATCCCATGCGTGGATCGCAGGTGCCGC
TCGTGCCAATTCCTCGGCTGAAACCCCCGCAGCCTGCATCTGCTGTAGCAGAGCCACCGGCCAAGAAGGCTAGGAAGACC
AAGAAGGTTGCAAGTGTTAGTCTCCTGGGGTTGATTTGCGTTGCAATGCTTTGTGGGTGTTTGATTCCTGCAGTAAATCG
GATGTATGACTCTGTTGATGCTGGAGAAGGTGCTGCATACGGTCCATCTCATCGTGGGAGGGTGCTGGCTGTTGAGGGGC
CTCATGATAATGTTTCAGATGGTGTCGATCCAAAGCCGCCACAGCGTGCCAGTGAGACGCTTCCAGCACTGTTGTATCTA
CCAAAGAATGGGAAGCATGTCAAGATTAATGGGAATCTTGTTATTAAGTCGATTGTTGCTAGTGAGAAAGCTTCATTGCA
GATGTCTGGCAATGATGGGAAGCGTCCTCAAAACCAAGAGGAGGAGACTAGACTGGCAATTCCTGGCTATGTGACTCCAT
TGAAAGCTGGAGAGGTTATGGAATCAACCGAAGGAATGATGAACAATGAACTGTTGGCGTTAGCTCCTGCAGATGGAAGC
ATGTATAGGGAGGAGGATGGATTGCTGCCACAGTGGTTTAGTGAAGCAATGTCTGGCCCCCTGCTGAGCTCAGGAATGTG
CACTGAAGTTTTCCAGTTCGATGTGTCCCCATCATCTGCTCATCCAAATGGCATCATTCCTGTCTATTCCAATGCCATGT
CAAACTCGTCACAGAACTTCACTCAAGATCTTCCCCCTGCTCGTGTTCGCACGGTCAAGAACAGAAGGATTTCGTACTCC
GAGGCCATTCCTCTGAGAGGTTCAACATCCAACGACACTGAGCACCTCAAGAGCTTTGGCAGTACGAAGCCTGTTTCGTC
AGTGGTCGTCTCTGTGCTGGCTGATCCCAGAGAGGCTGGCGATGGGGATGGTGAGGGAAGGATCTCTTCAAAATCATTGT
CCCGCATCTTAGTTGTCGTTCTTATAGATAGTGTTAAGTATGTTACGTACTCTTGTGTCCTGCCGTTCAAAAGCCATAGC
CCTCACCTT
TFome clones do not contain stop codon at 3'end.
Translation: MAEPALLPPDPFADLPFPEFQAPIDGDSFALEDFDLEDLDLDVDFDLDLFGSDGQPSQPPPLATSSSSAGSPVGGSSSSG
AVGDGGGLRNEESSESSSRSASGTDGSGKGKGEEDEAKRRARQVRNRESAHLSRQRKKQYVEELEGKVKAMQATIADLSA
RISCVTAENAALKQQLGGAAGAAPPPMPMYPTMYSLPMPWMHPSYPMRGSQVPLVPIPRLKPPQPASAVAEPPAKKARKT
KKVASVSLLGLICVAMLCGCLIPAVNRMYDSVDAGEGAAYGPSHRGRVLAVEGPHDNVSDGVDPKPPQRASETLPALLYL
PKNGKHVKINGNLVIKSIVASEKASLQMSGNDGKRPQNQEEETRLAIPGYVTPLKAGEVMESTEGMMNNELLALAPADGS
MYREEDGLLPQWFSEAMSGPLLSSGMCTEVFQFDVSPSSAHPNGIIPVYSNAMSNSSQNFTQDLPPARVRTVKNRRISYS
EAIPLRGSTSNDTEHLKSFGSTKPVSSVVVSVLADPREAGDGDGEGRISSKSLSRILVVVLIDSVKYVTYSCVLPFKSHS
PHL
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map