TFome Information for pUT3462

TFome Name:pUT3462
Genbank Insert ID:KJ726917
Protein Name:ZmbZIP46
Species: Maize
TF Family: bZIP
Template: BT087970.1
Corresponding TF Gene: GRMZM2G037910
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B541_F 3' Primer Name: B541_R
5' Primer Sequence: caccATGGACGAGGACTGGAGCAC 3' Primer Sequence: ATGGTTCCACTGCTTCTGCTGAG
5' Primer Tm (º C): 69.1 3' Primer Tm (º C): 65.9
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGACGAGGACTGGAGCACCGATCCCGCTCACCGCCGACGTCGCCTCCTCTTGCCGACGAGCTGTCAGACCCAGACCCA
GAACCCGCCACAGACACAGCCCCCGCTCCCCATGGACCTCGCCTCCCAGTACAACCGACGCTTTGCACCGTCACTTTTCC
TCCCACCGATGGCGCCCCGCCTGGCTTCCAGCTCCGGCTTCTCGGTCTTCAGCAACTACCAGAGCCCGCCGACGCTCGTG
CCCACGGCGGCGGGCGCCGGATCGCATCTCGCTCGGTCGCTCCCGAAGCTGCCTCTTTTCTCCATGGACTCTCTAGGTCT
TCTCCCGTACTCCACCAATCCTGCGGCGGGGGCGGCGGTCCCGCGTTCCCTGTCGTCGCCTGGCGGCTCCGAGCAGCAAG
TGCCGTCCGCGTCCGGCCTGCCGCCGCGCGGGGCGGGGCACCGGAGGTCCCGTAGCGATTTCCTCGTCGGGTTCTCGCGC
CCGAATCAGCTCTCCCTGCCGATGACTCCTGCAGCTGGGGAATACAGGTCGAGGGAAGCCTCCGTACTCGAGGAGCTATT
CCGCTCGTACAGGGACCCGAATCTCAGCTCCTCCGGGGATAACAACAGTGAAAGGAACGATCACTTAGGCAGACAACTCA
CCGGCCAAGGCGCTTGGAGCCCCGGTGATAGCAGCGACAATGACGCTGAGAGCTGGGCGGCTAGTGGCAGCGCAGCCGCA
GGCACCAGCGCTAGCCATAGCCATACTCGCCATTGCCGCAGCCTGTCGGTGGACAGCATCATGGGCAATCTCAACTTTGG
AGGCCTGGGCCAGGTTTCTCTGAGACTGCCGCCTCCGTCTCCGGTGGCAGGCGCCGGCGGCAGCCTCTCGCGTGCTGGAA
GTGGGGCATCGGGCGGTGCGGTTGCGACTGCCTCTTCTCAACCTGCCAACGGAGAGTTCAGCGAGTCTGAGATGAAAAAG
ATCATGGCCAATGATCGCCTTGCTGAGCTCGCTCTTGTTGATCCTAGGAGGGTCAAGAGGATTCTAGCTAATCGGATCTC
GGCAGCGAAGTCTAAGGAGCGCAAGGTGAAGTACATGGGTGAGCTTGAGCGCAAGGTTCGTGTGCTGCAGATGGAAACTA
GTACGTTATCTTCAAAAGCAGCATCGTCGCAGAGGGAATGCGAGGCACTTAGAATTCTGAACAATGAGATGAAGATCAGG
CTGCAAGCAATGGAGCAGCAAGCACAGCTGAAAGATGCTCTGAATGAAGCACTGACTGCTGAAGTGCAGCGCCTGAAACA
AATTGCTGGTGAGGCCAGTGATCCTCATGTGCCGAATATGAGCCGCAAGATTGTCGAACAGCAGCTGCAGAAGCAGCCAT
CGGAGGCCCAGCTGGCTGAGCAGCAGCAGCCACAGGAATCAGAGGAGTTCAAAGCTCAGCAGAAGCAGTGGAACCAT
TFome clones do not contain stop codon at 3'end.
Translation: MDEDWSTDPAHRRRRLLLPTSCQTQTQNPPQTQPPLPMDLASQYNRRFAPSLFLPPMAPRLASSSGFSVFSNYQSPPTLV
PTAAGAGSHLARSLPKLPLFSMDSLGLLPYSTNPAAGAAVPRSLSSPGGSEQQVPSASGLPPRGAGHRRSRSDFLVGFSR
PNQLSLPMTPAAGEYRSREASVLEELFRSYRDPNLSSSGDNNSERNDHLGRQLTGQGAWSPGDSSDNDAESWAASGSAAA
GTSASHSHTRHCRSLSVDSIMGNLNFGGLGQVSLRLPPPSPVAGAGGSLSRAGSGASGGAVATASSQPANGEFSESEMKK
IMANDRLAELALVDPRRVKRILANRISAAKSKERKVKYMGELERKVRVLQMETSTLSSKAASSQRECEALRILNNEMKIR
LQAMEQQAQLKDALNEALTAEVQRLKQIAGEASDPHVPNMSRKIVEQQLQKQPSEAQLAEQQQPQESEEFKAQQKQWNH
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map