TFome Information for pUT3489

TFome Name:pUT3489
Genbank Insert ID:KJ726943
Protein Name:ZmbZIP68
Species: Maize
TF Family: bZIP
Template: BT039652.1
Corresponding TF Gene: GRMZM2G157722
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C246_F 3' Primer Name: C246_R
5' Primer Sequence: caccATGGATCTCAACGAATGCGA 3' Primer Sequence: CCAGGGACCTGTCAATGTTCTCC
5' Primer Tm (º C): 66.1 3' Primer Tm (º C): 65.5
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 40 sec 72°C 7 min 72°C leave at 4°C for clones:
Nucleotide Sequence: ATGGATCTCAACGAATGCGAGAGGAGGGGGCCGGCGGGGCCGGATCCGCTGTCGAGGCAGGGGTCGATCTACTCGCTCAC
GTTCGACGAGTTCCAGAACACGCTCGGCGGGATGGGGGGAGGGCTTGGCAAGGACTTCGGCTCCATGAACATGGACGAGC
TGCTGCGGAGCATCTGGACCGCCGAGGAGAGCCAGGCCATGGCGTCGGCTTCGGCCTCGGCCTCGGCCTCGGTGTCCGCG
CTGGGGGCAGCCGTCGACGGCGGGGCGGCGCTGCAGAGACAGGGGTCTCTCGCGCTGCCGCGCACTCTCAGCGTCAAGAC
GGTGGACGAGGTCTGGCGGGACTTCGTGCGGGAGGCGCCGCCCGGGACGGCTGGCGGCGAGGAGCCGCAGCCCAACCGAC
AGCCGACTCTCGGGGAGATGACGCTGGAGGAGTTCCTGGTCAGGGCCGGGGTCGTCCGTGACAACCCTGCCGCGGCAGCT
GCTGCTGTCCCTGCGCAGCCGGTTGCTCCACGGCCGATTCAGGCGGTCAGTAATGGCGCCTCCATCTTTTTCGGCAACTT
TGGGGGCGCCAATGACGCCGGGGCTGGAGCGATGGTGTTTGCGCCGGTGGGGATCGGGGATCAGGCCATGGGCAACGGGT
TGATGCCTGGGGTGGCTGGGATGGCAGGTGGTGCGGTCACCGTGAGTCCGGTGGATACGTCGGTGGCCCAGCTGGATTCT
ATGGGCAAGAGCGATGAGGATCTGTCATCGCCAATGGCTCCGGTGCCATACCCCTTTGAGGGAGTGATAAGGGGAAGGAG
GAGCGGTGCGGGTGTGGAGAAGGTTGTGGAGCGGCGGCAGAGGAGGATGATCAAGAACAGGGAGTCTGCAGCTAGATCCC
GTGCCCGTAAGCAGGCTTATACAATGGAGTTGGAAGCCGAAGTTCAGAAACTGAAGGAACAAAATGCAGAATTGCAGAAG
AAGCAGGAACAAATAATGGAGATGCAGCAAAACCAGGTCCCGGAATTGGTCAGCAATCCATATGCACAAAAGAAGCGATG
CCTGCGGAGAACATTGACAGGTCCCTGG
TFome clones do not contain stop codon at 3'end.
Translation: MDLNECERRGPAGPDPLSRQGSIYSLTFDEFQNTLGGMGGGLGKDFGSMNMDELLRSIWTAEESQAMASASASASASVSA
LGAAVDGGAALQRQGSLALPRTLSVKTVDEVWRDFVREAPPGTAGGEEPQPNRQPTLGEMTLEEFLVRAGVVRDNPAAAA
AAVPAQPVAPRPIQAVSNGASIFFGNFGGANDAGAGAMVFAPVGIGDQAMGNGLMPGVAGMAGGAVTVSPVDTSVAQLDS
MGKSDEDLSSPMAPVPYPFEGVIRGRRSGAGVEKVVERRQRRMIKNRESAARSRARKQAYTMELEAEVQKLKEQNAELQK
KQEQIMEMQQNQVPELVSNPYAQKKRCLRRTLTGPW
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map