TFome Information for pUT3491

TFome Name:pUT3491
Genbank Insert ID:KJ726945
Protein Name:ZmbZIP61
Species: Maize
TF Family: bZIP
Template: BT063353.1
Corresponding TF Gene: GRMZM2G137046
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C255_F 3' Primer Name: C255_R
5' Primer Sequence: caccATGCAGGAGCAGGCGGC 3' Primer Sequence: TTGGCCGTGGCCCTCTCCA
5' Primer Tm (º C): 71.1 3' Primer Tm (º C): 69.7
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 40 sec 72°C 7 min 72°C leave at 4°C for clones:
Nucleotide Sequence: ATGCAGGAGCAGGCGGCGAGCTCGCGGCCTTCCAGCAGCGAGAGGTCGTCCAGCTCCGGGCACCACGTGGACATGGAGGT
CAAGGAAGGGATGGAGAGCGACGATGAGATAAGGAGAGTGCCGGAGCTGGGCCTGGAGTTGCCGGGAGCCTCCACGTCGG
GCAGGGAGGCTGGCCCTGGCGCTGCGGGCGCAGACCGCGCCCTTGCCCAGTCGTCCACGGCGCAGGCCAGCGCGCGCCGC
CGCGTCCGCAGCCACGCCGACAAGGAGCACAAGCGCCTCAAAAGGTTACTGAGGAACAGGGTGTCAGCTCAACAGGCTAG
AGAGAGGAAGAAGGCTTATTTAACTGATCTGGAGGTGAAGGTGAGAGATCTGGAGAAGAAGAACTCGGAGATGGAAGAGA
GGCTCTCCACCCTCCAGAACGAGAACCAGATGCTCCGACAGATACTGAAGAACACCGCTGTAAACAGAAGAGGTTCAGGA
AGCACTGCTAGTGGAGAGGGCCACGGCCAA
TFome clones do not contain stop codon at 3'end.
Translation: MQEQAASSRPSSSERSSSSGHHVDMEVKEGMESDDEIRRVPELGLELPGASTSGREAGPGAAGADRALAQSSTAQASARR
RVRSHADKEHKRLKRLLRNRVSAQQARERKKAYLTDLEVKVRDLEKKNSEMEERLSTLQNENQMLRQILKNTAVNRRGSG
STASGEGHGQ
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map