TFome Information for pUT3644

TFome Name:pUT3644
Genbank Insert ID:KJ726950
Protein Name:ZmARF33
Species: Maize
TF Family: ARF
Template: BT064253.1
Corresponding TF Gene: GRMZM2G179121
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2994up 3' Primer Name: UT-2994dn
5' Primer Sequence: caccATGGCGACGCAGCAACCG 3' Primer Sequence: GGAGGAGGGAGGAGCTCGATTCA
5' Primer Tm (º C): 61.9 3' Primer Tm (º C): 62
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 61C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGCGACGCAGCAACCGCTTCCCCCCGCCGACGGCGTCGGCGATAACACCGTCGACCGCGACGTCTGGCTGGCCTGCGC
CGCACCGTTTTCCCGCATCCCCACCGTCGGCGACGAGGTCTACTACTTCCCCGACGGCCACATCGAGCAGCACCAGCACC
TGTCCGCGGCGCCGCTCCCTGCGCAGGACCGCTTCCACTGCACCGTCACCGATGTGAGCCTCGGCGTGGACGACAAGACG
GACGAGGTGTTCGCCAAAATCTCCCTCAGACCACGGCCAGGCCGCGCCGCCGCTCCTCCTCCGGGCCCCGGCGGCAGCAG
CAACAGCCCCGCGCCCGCCCCCGGCCCGCCACAGAAGCTCAGATACTTCACTAAGGATCTCTCGCAAACCGACGTCTACG
CAAAGTTCCGCATCCCGCTGGAGAACGAGCACGTCTTACCCATACCCAAGGTCGAAACGGACGGTGCCGACCAGCAGCGC
GTGCAGCGCCAGGACGTCGTCATGCGCGACACCAGGGGCAAATCGTGGAGGTTCTCCGAAACCTACCGCGTCAATCCGAG
CAAGGAACACTCGCTCGGAACCGGGTGGTTGGATTTCGCCAAGGCCAAGCGGCTCGCCGCGGGGGACAAGATCGTCTTCA
TGCGCCGCCCCAACGGCGACCTCATCGTCGGCGTGCGGCGCCTCCACGTGCCCAGGTACCGGCCGTTCGACTTCCAAGGC
CCGGCCCAGGACGTCATGGAGGCCGTACGCCTCGCCGCCGCGGGAAGGCCCTTCACAGTCACCTACTTCCCGAGACAGGC
CGCCGTGGAGTTCATCGTCCCGCGCAGCGAGGTGGATGACGCGCTCGCCACCAGCTGGGAACCAGGCGCAGTGGTGCGCA
TGGAGGTGATGGAGGACGAGAACAGGCAGCACACCGTGTGGGTCCACGGCCGCGTCAATGCGATCCGCCAAAACATCTGG
CGAATGCTCGAGATCATCTGGGGTGTTGATCCTCCTCTTGCAACGACGAGATCTGTGAATGCTTGGCAAGTTGCGTCCCT
TCCTCCTCTTGCAACGACTGGGAGGAGTGTGATCCGGTCGGAATCAGAAAGCCAGGCCCAGAGCCCTTCTTCTTCTACTC
GAGCCAGCAGGCACACCGACAGTGGCCCTTCTGCTACACCCTTTTCTGCAACGCCACCGCGTACAATAAGCATCCCCAGT
GCTTCTCCCCCCAACAACCCAGTGAGCTTGTCTCCACTCCAGAACGTGAAGACCATAAAGCTCTTTGGCGAAACAATCTC
TGTTGATGGTGGTGGTGGTGGTGGTTCTGAACAAGTGAATCGAGCTCCTCCCTCCTCC
TFome clones do not contain stop codon at 3'end.
Translation: MATQQPLPPADGVGDNTVDRDVWLACAAPFSRIPTVGDEVYYFPDGHIEQHQHLSAAPLPAQDRFHCTVTDVSLGVDDKT
DEVFAKISLRPRPGRAAAPPPGPGGSSNSPAPAPGPPQKLRYFTKDLSQTDVYAKFRIPLENEHVLPIPKVETDGADQQR
VQRQDVVMRDTRGKSWRFSETYRVNPSKEHSLGTGWLDFAKAKRLAAGDKIVFMRRPNGDLIVGVRRLHVPRYRPFDFQG
PAQDVMEAVRLAAAGRPFTVTYFPRQAAVEFIVPRSEVDDALATSWEPGAVVRMEVMEDENRQHTVWVHGRVNAIRQNIW
RMLEIIWGVDPPLATTRSVNAWQVASLPPLATTGRSVIRSESESQAQSPSSSTRASRHTDSGPSATPFSATPPRTISIPS
ASPPNNPVSLSPLQNVKTIKLFGETISVDGGGGGGSEQVNRAPPSS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map