TFome Information for pUT3657

TFome Name:pUT3657
Genbank Insert ID:KJ726959
Protein Name:ZmbHLH65
Species: Maize
TF Family: bHLH
Template: BT084779
Corresponding TF Gene: GRMZM2G387528
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3282up 3' Primer Name: UT3282dn
5' Primer Sequence: caccATGTCCGACAGCAGCGACTTCG 3' Primer Sequence: TGTTTCAGCCTCATTTCTTCCTGTACAA
5' Primer Tm (º C): 65.1 3' Primer Tm (º C): 58.4
PCR Condition:
Nucleotide Sequence: ATGTCCGACAGCAGCGACTTCGCCGAGCTGCTATGGGAGAACGGCCAGGCGGTGGTGCACGCGCGGACGAGGAAACACCC
GCCCCAGCAGGCCGCCTTCCCGCCCTTCACCTTCACCTGCAGCAGCAGAGCTCAACAAAGGCAGCCGGTGCCGCCGCTGC
TCAAGACGGGAGGAGGTCTCTTTGCCGACTTCTCCTCGGCCACCGCCGGCCTAGACGCCCCCCGTGACAACGCCGACCTC
GACGACACCGTGCCGTGGATCCACTACCCCATCCCCATCGTCGACGAAGCCAGTCCCGCCGCGCCTGCCCTGGCAGATAG
CTTCATCCCAGATTTCTTCTCGGAGCTGCATGCGGCGGCGGCGACGACGAGCCTCGGCCCTCTGCCACCACCACCACCAC
CACCACCAGCAGTCCAGCACACCGGCGACGACAACGACAACCGGAGCACCCCGGTTCCCAATCCCACCACCGGCAGAGGA
CCAGAGCCCTCAAAGGAAACCCACCGCGCACCAGTACCAGGTCCAGCCGGCAGGCCTGAGCCACAACCACAAGCTGAGCT
CGCGCCCGCCAGGAAGCCTCGGCAGGAGAGCGGCGGCGGGGAGGCTTTGATGAACTTCTCCATCTTCTCTAGGCCGGCAG
CCATGGCGCGTGCCAGCCTGCGGCAGAGGCCGCCACAGACAGGCACCGACAAGGCGTCCAATGCCACCACGAGCACCCGC
GTGGAGTCGACGGTGCTACAGTCGGCCAGTGCCAGTGGGCCGAGAACTGCAGCTGCCCCCGCCGTGTTCGTGGACCAGAG
GACGGCGTGGCCGTCGCAGCAGCCCAAGGAGGTGCGGTTTCCATGCGCAGCAGCAGCGCCACCGCCGACGCCGACGGCTG
GTAACCTGCAGCAAGAAAGACCTAGCAACAACAACATGACTCCTCCGCAGAAAGAAGTAGAGACCAGGAAGGCGTCAGAG
GCTGCGGGCGCCACCGCCACCTCATCGGTCTGCTCCGGCAATGGTGCTGGGACAGGAAAGGACGACGAGTCCTGGCGCCA
ACAGAAGAGGAAGACCCTGCAGGCCGAGTGTTCCGCGAGTCAAGATGATGATCCTGACGATGAGTCCGGTGGGATGAGAA
GATCGTGCAGCAGAGGTGCGAAGCGCAGCCGCACCGCGGAGGTGCACAACCTGTCCGAAAGGAGGAGAAGGGACAGGATC
AATGAAAAAATGCGTGCCCTGCAAGAGCTCATCCCCAACTGCAACAAGATTGACAAGGCCTCCATGCTGGACGAAGCGAT
CGAGTACCTCAAAACCCTCCAGCTTCAAGTGCAGATGATGTCCATGGGAAGTGGGCTGTGCATTCCTCCCATGCTGCTGC
CACCAGCCATGCAGCACCTGCAGATTCCTCCGGCTGCTGCTCACTTCCCTCATCTCGGCATGGGATTGGGTTACGGGATG
GGTGTCCTCGATATGAACAGCGCGGCGGCGGCGGCGGCGGCGGTTCCATTTCCGCACATGCCCGGTGCTCACTTCCCTTG
CCCGATGATCCCAGGCGCGCCACTACCACGGGGTCTCGGAATCGGAATGCCTGCCAGAAACACGATGCCGGTGTTCGGAG
TCCCTCCTGGGCAACCAATCCAGCCACCATTCCCATCTTTGGCGGGCCTTCCTGTCAGGCCAAACCTGGCACCTCCTCAC
GCCTCCTCAGCCGCCATGGCTAACATGGTGCAGGAGCAACAGCAGCAGCAGCAGCATCAGAGCCTGAATAATGGAGCATC
AACAGCAGCAGCAACAGCATCAGATCCTCAGACTATCGTGCAGGCTGAGAGACAGCATTTTAGTGTGCCCTCTTCAGCAC
AAACCGAGAGCGATCAGTTTTTGGATGGTGGTGGCAACAGGGCTATTTGTACAGGAAGAAATGAGGCTGAAACA
TFome clones do not contain stop codon at 3'end.
Translation: MSDSSDFAELLWENGQAVVHARTRKHPPQQAAFPPFTFTCSSRAQQRQPVPPLLKTGGGLFADFSSATAGLDAPRDNADL
DDTVPWIHYPIPIVDEASPAAPALADSFIPDFFSELHAAAATTSLGPLPPPPPPPPAVQHTGDDNDNRSTPVPNPTTGRG
PEPSKETHRAPVPGPAGRPEPQPQAELAPARKPRQESGGGEALMNFSIFSRPAAMARASLRQRPPQTGTDKASNATTSTR
VESTVLQSASASGPRTAAAPAVFVDQRTAWPSQQPKEVRFPCAAAAPPPTPTAGNLQQERPSNNNMTPPQKEVETRKASE
AAGATATSSVCSGNGAGTGKDDESWRQQKRKTLQAECSASQDDDPDDESGGMRRSCSRGAKRSRTAEVHNLSERRRRDRI
NEKMRALQELIPNCNKIDKASMLDEAIEYLKTLQLQVQMMSMGSGLCIPPMLLPPAMQHLQIPPAAAHFPHLGMGLGYGM
GVLDMNSAAAAAAAVPFPHMPGAHFPCPMIPGAPLPRGLGIGMPARNTMPVFGVPPGQPIQPPFPSLAGLPVRPNLAPPH
ASSAAMANMVQEQQQQQQHQSLNNGASTAAATASDPQTIVQAERQHFSVPSSAQTESDQFLDGGGNRAICTGRNEAET
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map