TFome Information for pUT3670

TFome Name:pUT3670
Genbank Insert ID:KJ726969
Protein Name:ZmbHLH82
Species: Maize
TF Family: bHLH
Template: gDNA
Corresponding TF Gene: GRMZM2G417164
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: Tup1 3' Primer Name: Tdn1
5' Primer Sequence: caccATGTCCCACATCGCGGTGGAGCG 3' Primer Sequence: TATGGCGGCGGCCATGGCTG
5' Primer Tm (º C): 68.4 3' Primer Tm (º C): 67.3
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 67°C 35 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,4ng/ul template.
Nucleotide Sequence: ATGTCCCACATCGCGGTGGAGCGCAACCGGCGGCGTCAGATGAACGAGCATCTCAAGGTGCTCCGGTCCCTGACGCCCGG
TCTCTACATCAAGCGCGGCGACCAAGCATCCATCATCGGCGGCGCCATCGAGTTCATCAAGGAGCTGCAGCAGGTGCTGG
AGTCGCTGGAGGCCAGGAAGAAACGGCGCAGCAGCGGCGGCGGCCACAGCTTCCTTACGGGTAGCCCCAGCCCCACGCCC
AGCCCGCGCTCCCACCTCCTCTCCTCCGTTTCCACCCCGTCGCCGCCTGTCATGATCAAGGAGCTCGCCGCCTGCTGCAA
CTCCGCCGTGGCCGACGTCGAGGCCAAGATCTCCGGCTCCAACGTCCTGCTGCGCACGCTCTCCCGCCGCAGCAGCATCC
CCGGACGACAGGCCGTCAGGCTCATCGCCGTGCTGGAGGGCCTCCACCTGGAGGTGCTCCACCTCAACATCAGCACCATG
GAAGACACCGTCCTCCACTCCTTGGTGCTCAAGATCGGCCTCGAGTGCCAGCTCAGTGTTGAGGATCTTGCCTACGAGGT
GCAGCAGATCTTCGGTCAGGACACGGCCGAGCAGAAGCCGCAGGAGGAGAATCATAATCAGCATATCATATACTCGTCAG
CCATGGCCGCCGCCATA
TFome clones do not contain stop codon at 3'end.
Translation: MSHIAVERNRRRQMNEHLKVLRSLTPGLYIKRGDQASIIGGAIEFIKELQQVLESLEARKKRRSSGGGHSFLTGSPSPTP
SPRSHLLSSVSTPSPPVMIKELAACCNSAVADVEAKISGSNVLLRTLSRRSSIPGRQAVRLIAVLEGLHLEVLHLNISTM
EDTVLHSLVLKIGLECQLSVEDLAYEVQQIFGQDTAEQKPQEENHNQHIIYSSAMAAAI
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>/John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map