TFome Information for pUT3673

TFome Name:pUT3673
Genbank Insert ID:KJ726972
Protein Name:ZmbHLH100
Species: Maize
TF Family: bHLH
Template: gDNA
Corresponding TF Gene: GRMZM2G313756
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: Tup4 3' Primer Name: Tdn4
5' Primer Sequence: caccATGGACATGGAGGAACAGTTGCTG 3' Primer Sequence: AATCAAACAATCAATCGCTGTTACAAGC
5' Primer Tm (º C): 58.9 3' Primer Tm (º C): 59.8
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59°C 35 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,4ng/ul template.
Nucleotide Sequence: ATGGACATGGAGGAACAGTTGCTGCACCTGGAGCACTTCATGCCCAGCAGCCCGGGCTTCTTTGACGTCGGCAGTGGCCA
CGACCCCGCCCACTTCCCGAACGGCGGGTTATTCGTCGAGCAGGTCGGCGCCACCGACGACGGCGGGTGGGTGGAGGAAC
TCATGCAGCTGGGCGAGGAGCTGTTCGGCGGTCCGGGCGATGGCGGCATCATGGGCGACGCCGACGACCACTACCAGCAG
TGGCAGTGCGACGACGACGTCGACGGCGGCTCCCCAGACGGCGGTCCGCCACCGAGCGTGTCCTTGGACGGCGACGACGC
GAGCCCAGGGGAGCAAGGCGCCGGGGAGCTCGCCTCCGAGCCGCACCGCGAGGACGGCGACGACGTGTCGGGCGCGACGC
GGAAGCGGAGGGACCGGTCCAAGACCATCGTGTCGGAGCGGAAGCGGAGGGTCCGGATGAAGGAGAAGCTCTACGAGCTC
AGGTCGCTCGTCCCCAACATCACAAAGGTGAGGCTAGCTCCGGTCTGGACTTCGGAGTTCGGACGACTGTTCGCTGGGGA
GCTCGGTCATTCGATTCATGTCGTCCCCCTGCATGCATGTGCGCGCCATGTGCAGATGGACAAGGCGTCCATCATCGCCG
ACGCGGTGGTGTACGTGAAGAACCTCCAGGCGCACGCCAGGAAGCTGAAGGACGAGGTCGCCACGCTGGAGGCCCGGCCG
AGATCGCCTACCCGACAGGACCAGCCGCAGCTGCAGCACGGCCGGAGCGCGGGGCCCGGCCGACGACGCCAGCAGCAGCA
GCAGGTGGAGCGCCGCGACGCGGGGAGCGCGGGCGCCGGCGCCCGGGTGACGCACGTGGGCGCGGCGCAGGTCGGCGACG
GCAGGTTCTTCGTGACCGTGGAGTGCGAGCGGCGGGACGGCGTGGCGGCGCCGCTCTGCGCGGCCGCCGAGTCGCTGCTG
TGCTTCCGGGTGGAGAGCTCCAGCATCGGCCGGTCGGGGCCGGACCGCGTCGTGTCCACGCTCACGCTCAAGGTCTGGAC
TGGACTGGACTGGACTGGATCCATGGGGTGGCTTGTAACAGCGATTGATTGTTTGATT
TFome clones do not contain stop codon at 3'end.
Translation: MDMEEQLLHLEHFMPSSPGFFDVGSGHDPAHFPNGGLFVEQVGATDDGGWVEELMQLGEELFGGPGDGGIMGDADDHYQQ
WQCDDDVDGGSPDGGPPPSVSLDGDDASPGEQGAGELASEPHREDGDDVSGATRKRRDRSKTIVSERKRRVRMKEKLYEL
RSLVPNITKVRLAPVWTSEFGRLFAGELGHSIHVVPLHACARHVQMDKASIIADAVVYVKNLQAHARKLKDEVATLEARP
RSPTRQDQPQLQHGRSAGPGRRRQQQQQVERRDAGSAGAGARVTHVGAAQVGDGRFFVTVECERRDGVAAPLCAAAESLL
CFRVESSSIGRSGPDRVVSTLTLKVWTGLDWTGSMGWLVTAIDCLI
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>/John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map