TFome Information for pUT3674

TFome Name:pUT3674
Genbank Insert ID:KJ726973
Protein Name:ZmbHLH113
Species: Maize
TF Family: bHLH
Template: gDNA
Corresponding TF Gene: GRMZM2G471635
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: Tup6 3' Primer Name: Tdn6
5' Primer Sequence: caccATGGACTTCGACATGCTGAATTCCC 3' Primer Sequence: CCACTGGCCGTTCATGGCAC
5' Primer Tm (º C): 61 3' Primer Tm (º C): 60.7
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 61°C 35 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,4ng/ul template.
Nucleotide Sequence: ATGGACTTCGACATGCTGAATTCCCACCCGGAGGCGCAGCTTGAGCTGATGAAAGCAATGCTCCAGCTGGAGCAGCTCAC
TGCGTTCCCTCTCCCTGATCACGGCGCCATGATGATGGTGCCTTCAACTCCCCCGTCGCCAGGCATGCCGCAAGCTACTA
CCCACCACCATTATTTCTCTTCGGTGCCGCACCACATGTCTGCGGGCGCCAACGGCCGTGGCGTTTACGACGACGACCAC
CAGTACTCCCAGGGCACAGCTGCGCCCTGCAACGGCAGCGCCCGGTCGTCGGAGTACGTGGCGCATGCGCAGGGCCCTGT
CGCCGGCGGCGGAAACAGCGGGGAGAGCACCGCCGCCACCTCCGTCGGGTCGTCGTCGTCGGCGATGCGGGAGATGATCT
TCCGCGTGGCTGCGCTCCAGCCCGTGAACATCGACCCGGAGATGGTGCGGCCCCCGAAGCGGCGCAACGTGCGCATCTCC
ACCGACCCGCAGAGCGTGGCGGCACGGATGCGGCGGGAGCGCATCAGCGAGCGCATCCGCATCCTGCAGCGGCTCGTCCC
GGGCGGCACCAAGATGGACACCGCGTCCATGCTGGACGAGGCCATCCACTACGTGAAGTTCCTCAAGACGCAGGTGCAGT
CCCTCGAGCGCGCCGCCGCCGCCAGCGGCCGCCGCCCGGCGGCACCTGCAGTGGCCGATAACGGTGCCATGAACGGCCAG
TGG
TFome clones do not contain stop codon at 3'end.
Translation: MDFDMLNSHPEAQLELMKAMLQLEQLTAFPLPDHGAMMMVPSTPPSPGMPQATTHHHYFSSVPHHMSAGANGRGVYDDDH
QYSQGTAAPCNGSARSSEYVAHAQGPVAGGGNSGESTAATSVGSSSSAMREMIFRVAALQPVNIDPEMVRPPKRRNVRIS
TDPQSVAARMRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKTQVQSLERAAAASGRRPAAPAVADNGAMNGQ
W
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>/John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map