TFome Information for pUT3675

TFome Name:pUT3675
Genbank Insert ID:KJ726974
Protein Name:ZmbHLH150
Species: Maize
TF Family: bHLH
Template: gDNA
Corresponding TF Gene: GRMZM2G045431
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: Tup8 3' Primer Name: Tdn8
5' Primer Sequence: caccATGGAGTTTGACCTGCTGAATTACAG 3' Primer Sequence: CATGTCGCCGTGGGGC
5' Primer Tm (º C): 56.8 3' Primer Tm (º C): 56
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 56.2°C 35 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 4ng/ul final concentration
Nucleotide Sequence: ATGGAGTTTGACCTGCTGAATTACAGCCCGGAAGCGCAGCTTGAGCTGATGACCACGATGCTCCAGCTGGAGCAGCTAAC
TGCGCTCGACGGCCATCAGTTTCCGACGATGGCGCCTGTCTCGCCGCCGATATCCCCGATGCAAACCCATCATGCAGGTC
ACAGCTTGTCGCCTCCGCCACACATGTCGGCGACGACGACGACCACCACCACCGGGTACCCAGAGCAGTACACGCCGGCG
GCGGCCGTGTACGGCGCCACCGCTACCGGCCTCGAGCACCTCCAGGACTACGTGCTGTCCCCGCCGGGCGGCGACTGCGG
CGGCGCGGCGGCGGCGCACGCGGGGTCGACGTCGCCGACGTCGGCGGACGCGATGCGGGAGGCGATCTTCCACATCGCCG
CGCTGCAGCCGGTGGAGATCGACCCGGAGGCGGTGCGTCCCCCGAAGCGGCGCAACGTGCGCATCTCCAAGGACCCGCAG
AGCGTGGCGGCGCGGCTGCGGCGGGAGCGCATCAGCGAGCGCATCCGCACCCTGCAGCGCCTCGTCCCGGGCGGCACCAA
GATGGACACGGCGTCCATGCTGGACGAGGCCATCCACTACGTCAAGTTCCTCAAGTCCCAGGTGCAGTCCCTGGAGCGCG
CCGCCCACCGCGCCGCCGCGCTCGGCGCCGCCGCCTACCCGGCCTTCCACGCGCCGTGGCAGTACGCGCTGCCCCACGGC
GACATG
TFome clones do not contain stop codon at 3'end.
Translation: MEFDLLNYSPEAQLELMTTMLQLEQLTALDGHQFPTMAPVSPPISPMQTHHAGHSLSPPPHMSATTTTTTTGYPEQYTPA
AAVYGATATGLEHLQDYVLSPPGGDCGGAAAAHAGSTSPTSADAMREAIFHIAALQPVEIDPEAVRPPKRRNVRISKDPQ
SVAARLRRERISERIRTLQRLVPGGTKMDTASMLDEAIHYVKFLKSQVQSLERAAHRAAALGAAAYPAFHAPWQYALPHG
DM
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>/John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map