TFome Information for pUT3688

TFome Name:pUT3688
Genbank Insert ID:KJ726987
Protein Name:ZmbHLH35
Species: Maize
TF Family: bHLH
Template: BT061358
Corresponding TF Gene: GRMZM2G173372
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3246up 3' Primer Name: UT3246dn
5' Primer Sequence: caccATGGAGCTTGACGAGGAGACGACG 3' Primer Sequence: TACAGTTTCCAGAATGAAAAAGGAATCCGATC
5' Primer Tm (º C): 62.3 3' Primer Tm (º C): 58.6
PCR Condition:
Nucleotide Sequence: ATGGAGCTTGACGAGGAGACGACGTTCTTGGACGAGCTCATGTCGCTGCGGCGGGAGGCGGCGGCGGCGGCGTCCGCGCA
GTGGCAAGCAGCTGCCGCCTACACGGGCGGCGGCGGACAAGGCATGATGATGGGCGACCTCCTCTTCTACGGCGGGACGA
CGGAGGCCGCCGCGACTGCCACCAGCGGCGGCGGCATGGACCAGCTCTCGCCGTTCCACCAGCAGGAGCCGCTGCAGGTG
CAGGCGCCCCCGCACCCTCACTCGCACGAGGAGTTCAACTTCGACTGCCTCAGCGAGGTTTGCAACCCGTACAGGGGCGG
CGGCGTCGCCGTAATAACGGGGGCCGAGGCCGCCGCGGTGCCCGGAAGGGCGCTCGCCGCTCTCCACGACGCCATGGTGG
AGGACGAGACGAGCGGTCACCTGCAGGGGCAGCAATATGGAGCCGGCGGCTCCCCGACGTTCGTGTTCGGCGGTGCCGCC
GGCGAGAGCTCCGAGCAGACGCCCGTCGTCGTCAGGGGTGCCGCCGGCGGCCCCCACCACAGGAGCAAGGTCCACGGCGG
CGGCGCGCCGTCCAAGAATCTCATGGCCGAGAGGCGGCGGCGGAAGCGCCTCAACGACCGTCTCTCCATGCTCCGCTCTA
TTGTTCCCAAGATCAGCAAGATGGACAGGACATCCATTCTCGGTGACACCATTGACTACGTGAAGGAGCTGACGGAGCGG
ATCAAAGTCCTGGAAGAGGAGATCGGCGCCTCTGCGGAGGACCTGGACCTCCTCAACACCTTGAAAGCTTCTTCGTCCAG
CGGCAGCAACGAGATGATGGTGAGGAACTCCACAAAGTTCGACGTCGAGAGGCGGGGCAACGGGAGCACGAGGATCGAGA
TCTGCTGCGCCACAAACCCCGAGGTGCTGCTGTCCACGGTAAGCGCGCTGGAGGTGCTGGGCCTGGAGATCGAGCAGTGC
GTGGTGAGCTGCTTCAGCGACTTTGGCATGCAGGCCTCTTGCTCACAAGTGAGTAGTAGTACTACATGCATGGTTAAGGG
ATCGGATTCCTTTTTCATTCTGGAAACTGTA
TFome clones do not contain stop codon at 3'end.
Translation: MELDEETTFLDELMSLRREAAAAASAQWQAAAAYTGGGGQGMMMGDLLFYGGTTEAAATATSGGGMDQLSPFHQQEPLQV
QAPPHPHSHEEFNFDCLSEVCNPYRGGGVAVITGAEAAAVPGRALAALHDAMVEDETSGHLQGQQYGAGGSPTFVFGGAA
GESSEQTPVVVRGAAGGPHHRSKVHGGGAPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYVKELTER
IKVLEEEIGASAEDLDLLNTLKASSSSGSNEMMVRNSTKFDVERRGNGSTRIEICCATNPEVLLSTVSALEVLGLEIEQC
VVSCFSDFGMQASCSQVSSSTTCMVKGSDSFFILETV
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map