TFome Information for pUT3689

TFome Name:pUT3689
Genbank Insert ID:KJ726988
Protein Name:ZmbHLH168
Species: Maize
TF Family: bHLH
Template: BT064056
Corresponding TF Gene: GRMZM2G030744
Transcript: T03
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3303up 3' Primer Name: UT3303dn
5' Primer Sequence: caccATGGACGCGCAGCAGCAGC 3' Primer Sequence: GTTTGATCCTGCCCTCAGACCTCC
5' Primer Tm (º C): 67.6 3' Primer Tm (º C): 61.3
PCR Condition:
Nucleotide Sequence: ATGGACGCGCAGCAGCAGCTGGATTTGGTCATGCGTCACCAGAGCATGGCCACCGTCTGCGAGAGCGAGGACGCGCTGGG
GTCGTCGGAGTCGGATCCCGCGAGGCCGGCGCGCCCGCGCGGCAAGAGGAGCCGCGCCGCCGAGGTGCACAACCTCTCCG
AGAAGAGGAGGAGGAGCAGGATCAACGAGAAGATGAAGGCGCTGCAGACCCTCATACCCAACTCGAGCAAGACGGACAAG
GCTTCCATGCTTGATGATGCCATTGAGTACCTGAAGCATCTTCAGCTCCAGGTGCAGATGTTGTCTATGAGGAATGGCCT
CTATCTGCCCCCAGGAAACCTGTCTGGAGCACCCGAGACTCTGGCACCCTTAGAAATGTGCGCAGCACTTAACCAAAGTG
GTGCCAAGGCATCAAATTCCGGCGTTGTCGTTCTACCTGGAAACAAAATTCCTGTAGCTCGTCTTCTACTTGATCCACCA
AACCATGATCAGCGGCATGAAAACCCGCTTGTATTACAAAGTGTTCCTAGTAGTTCGACCGCTGTAGAGCCTCGGTTCCT
TCAACAGCCAGCACAGCCCAACCTCCAATCCTTTCAGCTGGCTGTACCTCCCGAGATGATCTTCGACGAGGCGGACATGA
TGCTAAAGCATCGCCTAGCTTCAGGTCGAGAAACCACATCTCTACCAGTAGGACATGAGGCCAAGCCTGGTAGGCAGGAA
GCATGCATGGTAAATTCTGATATTTCTAACAGAGGTTCACTCGGGAAAGAAGTATTTATGCCCTACTTGCATAGCTTGCA
GAGCGGCGATACGGAGGGAGGTCTGAGGGCAGGATCAAAC
TFome clones do not contain stop codon at 3'end.
Translation: MDAQQQLDLVMRHQSMATVCESEDALGSSESDPARPARPRGKRSRAAEVHNLSEKRRRSRINEKMKALQTLIPNSSKTDK
ASMLDDAIEYLKHLQLQVQMLSMRNGLYLPPGNLSGAPETLAPLEMCAALNQSGAKASNSGVVVLPGNKIPVARLLLDPP
NHDQRHENPLVLQSVPSSSTAVEPRFLQQPAQPNLQSFQLAVPPEMIFDEADMMLKHRLASGRETTSLPVGHEAKPGRQE
ACMVNSDISNRGSLGKEVFMPYLHSLQSGDTEGGLRAGSN
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map