TFome Information for pUT3963

TFome Name:pUT3963
Genbank Insert ID:KJ726991
Protein Name:ZmCA3P3
Species: Maize
TF Family: CCAAT-HAP3
Template: gDNA
Corresponding TF Gene: GRMZM2G169884
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: Tup24 3' Primer Name: Tdn24
5' Primer Sequence: caccATGAGCCATACTTCCAATTTCACTG 3' Primer Sequence: ATCTCTGCCACCTTGCTGGTTG
5' Primer Tm (º C): 55.5 3' Primer Tm (º C): 58
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 56.2°C 35 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, gDNA template 4ng/ul final concentration
Nucleotide Sequence: ATGAGCCATACTTCCAATTTCACTGGTTTTTCCCAATTAGAACATCCCCAACCCCAAAGAAATTCTAGGGCATCAAGTTC
AACCACACATGATGCCAACGTTAGGCATGACAACAACCTCTTGCCCATTGCCAATGTCGGGAGGATCATGAAGGACGCCC
TCCCTCCGCAAGCCAAGATCTCGAAGCATGCCAAGGAGACCATCCAAGAGTGCACGACGGAGTTCGTGGGCTTCGTCACC
GGCGAGGCCTCCGAGCGGTGCCGCCGGGAGAGGCGGAAGACCATCAACGGCGACGACATCTGCCATGCCATGAGGAGCCT
TGGCCTCGACCACTACGCCGACGCCATGCGCAGGTACCTGCAGAGGTATCGTGAGACCGAGGAGCTAGCGGCAGCACTCA
ACAGTGGCGGTGGTGGCCACGATGGCAATGCCATTCAGATTGATGTGAGGGATGAGTTGTCCATCTTCAAGGGTAGCAAC
CAGCAAGGTGGCAGAGAT
TFome clones do not contain stop codon at 3'end.
Translation: MSHTSNFTGFSQLEHPQPQRNSRASSSTTHDANVRHDNNLLPIANVGRIMKDALPPQAKISKHAKETIQECTTEFVGFVT
GEASERCRRERRKTINGDDICHAMRSLGLDHYADAMRRYLQRYRETEELAAALNSGGGGHDGNAIQIDVRDELSIFKGSN
QQGGRD
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map