TFome Information for pUT3964

TFome Name:pUT3964
Genbank Insert ID:KJ726992
Protein Name:ZmCA5P16
Species: Maize
TF Family: CCAAT-HAP5
Template: gDNA
Corresponding TF Gene: GRMZM2G078691
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: Tup30 3' Primer Name: Tdn30
5' Primer Sequence: caccATGGATCCTAACAAATCCAGCACCCC 3' Primer Sequence: CTGCTGAGGCACGTAGTAGTAGGCGAGAG
5' Primer Tm (º C): 63 3' Primer Tm (º C): 64.3
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 63.4°C 35 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, gDNA template 4ng/ul final concentration
Nucleotide Sequence: ATGGATCCTAACAAATCCAGCACCCCGCCGCCGCCTCCAGTCATGGGTGCCCCCGTTGCCTACCCTCCGCCTGCGTACCC
TCCCGGTGTGGCCGCCGGCGCCGGCGCCTACCCGCCGCAGCTCTACGCACCGCCGGCTGCTGCCGCGGCCCAGCAGGCGG
CGGCCGCGCAGCAGCAGCAGCTGCAGATATTCTGGGCGGAGCAGTACCGCGAGATCGAGGCCACTACCGACTTCAAGAAT
CACAACCTCCCGCTCGCCCGCATCAAGAAGATCATGAAAGCCGACGAGGACGTCCGCATGATCGCCGCCGAGGCTCCCGT
GGTGTTCGCCCGGGCCTGCGAGATGTTCATCCTCGAGCTCACCCATCGCGGCTGGGCGCACGCCGAAGAGAACAAGCGCC
GCACGCTCCAGAAATCCGACATTGCCGCTGCCATCGCCCGCACCGAGGTATTCGACTTCCTTGTGGACATCGTTCCGCGC
GACGACGGTAAAGACGCTGATGCGGCGGCCGCCGCAGCTGCCGCGGCTGCCGGGATCCCGCGCCCCGCCGCCGGAGTACC
AGCCACCGACCCTCTCGCCTACTACTACGTGCCTCAGCAG
TFome clones do not contain stop codon at 3'end.
Translation: MDPNKSSTPPPPPVMGAPVAYPPPAYPPGVAAGAGAYPPQLYAPPAAAAAQQAAAAQQQQLQIFWAEQYREIEATTDFKN
HNLPLARIKKIMKADEDVRMIAAEAPVVFARACEMFILELTHRGWAHAEENKRRTLQKSDIAAAIARTEVFDFLVDIVPR
DDGKDADAAAAAAAAAAGIPRPAAGVPATDPLAYYYVPQQ
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map