TFome Information for pUT3983

TFome Name:pUT3983
Genbank Insert ID:KJ727007
Protein Name:ZmCA5P14
Species: Maize
TF Family: CCAAT-HAP5
Template: gDNA
Corresponding TF Gene: GRMZM2G174776
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: Tup29 3' Primer Name: Tdn29
5' Primer Sequence: caccATGGAGCCCAACTCCAGCATCCCTC 3' Primer Sequence: CTGCGGCTGGACGTAGTAGTAGCCCATG
5' Primer Tm (º C): 66 3' Primer Tm (º C): 67
PCR Condition: Step 1, 98°C 10 sec melt, 1 cycle; Step 2, 98°C 30 sec melt, 66.0°C 35 sec anneal, 72°C 18 sec extend, 35 cycles; Step 3, 72°C 5 min extend 1 cycle; Step4, 4°C ; 1x GC (NEB), 3.5mM MgCl2, 0.2mMdNTP, 0.5uM ea primer, 7.5%glycerol, 3%DMSO, 0.02U/ul Phusion (NEB), 5ng/ul gDNA template
Nucleotide Sequence: ATGGAGCCCAACTCCAGCATCCCTCCCCCGGTGATGGGCGCGGCGGTGGCGTACCCTCCGGCGGCCGGCGCCGCGTACTC
CGCCGGGCCGTACGCGCACGCGCACGCGGCGTTGGGCGCGCTGTACCCGCCTCCCCCGGCGCCGGGTCCCCCCTCCTCGC
ACCAGGGCGGCGCGGCGGCGGCGCAGCTGCAGCTGTTCTGGGCGGAGCAGTACCGCGAGATCGAGGCGACGACGGACTTC
AAGAACCACAACCTGCCGCTGGCCCGCATCAAGAAGATCATGAAGGCGGACGAGGACGTGCGCATGATCGCCGCCGAGGC
GCCGGTGGTGTTCGCCCGCGCCTGCGAGATGTTCATCCTGGAGCTGACCCACCGCGGCTGGGCGCACGCGGAGGAGAACA
AGCGGCGCACGCTGCAGAAGTCGGACATCGCGGCCGCCGTGGCGCGCACCGAGGTCTTCGACTTCCTCGTGGACATCGTG
CCGCGGGACGAGCCCAGGGACGCCGACCCCGCCGCCGCCGCCATGGGGGCAGCAGCGCCCGGGATCCCGCAGCACCCCGC
CGCCGCCGACCCCACCACCATGGGCTACTACTACGTCCAGCCGCAG
TFome clones do not contain stop codon at 3'end.
Translation: MEPNSSIPPPVMGAAVAYPPAAGAAYSAGPYAHAHAALGALYPPPPAPGPPSSHQGGAAAAQLQLFWAEQYREIEATTDF
KNHNLPLARIKKIMKADEDVRMIAAEAPVVFARACEMFILELTHRGWAHAEENKRRTLQKSDIAAAVARTEVFDFLVDIV
PRDEPRDADPAAAAMGAAAPGIPQHPAAADPTTMGYYYVQPQ
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map