TFome Information for pUT3990

TFome Name:pUT3990
Genbank Insert ID:KJ727009
Protein Name:ZmCA5P1
Species: Maize
TF Family: CCAAT-HAP5
Template: gDNA
Corresponding TF Gene: GRMZM2G074773
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: Tup26 3' Primer Name: Tdn26
5' Primer Sequence: caccATGAGGCAGGCGAGGCCGTAC 3' Primer Sequence: GAGCGCGCCGTTGCCG
5' Primer Tm (º C): 62 3' Primer Tm (º C): 62
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 67.2°C 30 sec anneal, 72°C 12 sec extend, 35 cycles; Step 3, 72°C 5 min extend 1 cycle; 5ng/ul gDNA template, 1x GC (NEB), 3.5mM MgCl2, 0.2mMdNTP, 0.5uM ea primer, 7.5%glycerol, 3%DMSO, 0.02U/ul Phusion (NEB); 5ng/ul gDNA template
Nucleotide Sequence: ATGAGGCAGGCGAGGCCGTACTCGGGGATCTTCTGCGGCGGGGTGTCGGCGCGGACGGGACCGCACGCGCTGCCGCTGGC
GCGCATCAAGAAGATCATGAAGCGCTCCGCGGGGGAGGCGGCGGCCGCCGCGGACGGCGGCGCCAGGATGATCTCCGGCG
AGGCGCCCGTTGTGTTCTCCAAGGCGTGCGAGCTCTTCGTCGCCGAGCTCACGCGCCGCGCCTGGGCCGCCACGCTCGAC
GGCAAGCGCCGCACCGTGCACAGGGAGGACGTCGCCACGGCCGTGCACAACACCGACCTCTTCGACTTCCTCGTCGACGT
CGTCACGGCGGACACTGCTGGTGGAACCGGCGGCGGCGGGCACGACGGCAACGGCGCGCTC
TFome clones do not contain stop codon at 3'end.
Translation: MRQARPYSGIFCGGVSARTGPHALPLARIKKIMKRSAGEAAAAADGGARMISGEAPVVFSKACELFVAELTRRAWAATLD
GKRRTVHREDVATAVHNTDLFDFLVDVVTADTAGGTGGGGHDGNGAL
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map