TFome Information for pUT4131

TFome Name:pUT4131
Genbank Insert ID:KJ727067
Protein Name:ZmbHLH43
Species: Maize
TF Family: bHLH
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G165042
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGAACCAGTTCGTGCCGGACTGGTCCAACATGGGCGATACCTCTAGACCACTGGGCGAGGAGGACGACCTGATCGAGCT
TCTTTGGTGCAACGGCCACGTGGTGATGCAGAGCCAGAGCCATAGGAAGGTGCCACCGAGGCCAGAGAAGGCTGCTGCTG
TTGCTGCTCCACCAGCTCCAGCTTCAGTGCCACAAGAGGATGAGGGCGGCCTGTGGTTCCCATTCGCTCTGGCTGATAGC
CTGGACAAGGATATCTTCAGCGAGTTCTTCTGCGAGGCCCCAACTCCTGCTCCAGCTGCTGCAGACGCTGCCCCAGCTGC
TTCTGGCGGCGGCACTGGCACTGAGGCTGGCGGCAAGTCTTGCGGCGGGGATGTTCCAGTTCCAGCTGAGGATGATAGAA
GAGGCGGCGGCGGCGCTTGCGCTGTTTCTGCTGGCGATCCATGCGACCTGATGCCGCCACCAAAGTCTACCCCAGCTAGC
TGCTCTAGGCAGCAGACCACCATGTCTCTTGCTAACGGCGGCGATAACGCTGGCGGCGATCTTCCAGGCCTTGTTAGAGC
TGGGGCAGAGGCTGGCGCTAGCTCTATGCTTTCTGCTATCGGCAGCAGCATCTGCGGCAGCAACCAGGTTCTGGTTCAGA
GGGCTGCTTGCGCTCCAGGCAGAGCTTCTGCTTCTGGCTCTGGCACTGCTAGAGGGGATGGGTCAGGCTCTGCTGCTCTG
CCATCTGCTGTTGGCAGCGCCAACGCTAATGCTGTTGGCGGCGGCAGAGGCCATGAGGCTTCTAGCTCAGGCAGGTCCAA
CTACTGCTGCTTCGGCGCTGCTACCACCACCACAACCACCACTACAACCGAGCCAGCCAGCACCAGCAACAGGTCCTCTA
AGAGGAAGAGGCTGGACACCGAGGACAGCGAGTCTCCATCTGAGGATGCTGAGTCTGGCAGCGCCGCTATGCTTGCTAGA
AAGCCGCCACAGAAGATGACCACCGCTAGGCGCTCTAGGGCTGCTGAGGTGCACAATCTTTCTGAGAGGCGCAGGCGCGA
CAGGATCAACGAGAAGATGAGGGCCCTGCAAGAGCTGATCCCGCACTGCAACAAGACCGACAAGGCCAGCATGCTCGACG
AGGCCATCGAGTACCTGAAGTCCCTGCAGCTCCAGGTGCAGATGATGTGGATGGGCAGCGCTGGCATTGCAGCCCCACCA
GCTGTTATGTTCCCAGGCGTGCACCAGTACCTGCCAAGAATGGGCGTTGGCATGGGCGCAGCCGCTGCTGCTGCACTTCC
ATCTATGCCAAGGCTGCCGTTCATGGCCCCACAGCCAGTTGTTCCAAGCGCTCCAGTTTCTGTGGGCCCAGTGCCAGCTT
ACAGGGGCCATATGCCAGCTGTGGGCATCACTGAGCCATACGGCCATTACATCGGCGTGAACCACCTCCAGCCAGCTCCT
CCACCTCCACAGGTTCAAGGCGTGAGCTACTACCCACCACCACTGGGCGCTACTGCTAAGGCTGTTCAGCAGGCTGCTGA
GCTGCATCATGTTCCAGGCCCAGGCGGCTCTATTATGCCAGCTGGCGCTGCTCCAGGCGTGCTGCTTCCAGAGTCTGCTC
AAGGCAGAGGCCCAGGCACTGTGCCATGCGCTCCACCATTCTCTAGCAGCGCTTCAGTGTTCGGCCTGCAGATGGGCGCT
CTTAGA
TFome clones do not contain stop codon at 3'end.
Translation: MNQFVPDWSNMGDTSRPLGEEDDLIELLWCNGHVVMQSQSHRKVPPRPEKAAAVAAPPAPASVPQEDEGGLWFPFALADS
LDKDIFSEFFCEAPTPAPAAADAAPAASGGGTGTEAGGKSCGGDVPVPAEDDRRGGGGACAVSAGDPCDLMPPPKSTPAS
CSRQQTTMSLANGGDNAGGDLPGLVRAGAEAGASSMLSAIGSSICGSNQVLVQRAACAPGRASASGSGTARGDGSGSAAL
PSAVGSANANAVGGGRGHEASSSGRSNYCCFGAATTTTTTTTTEPASTSNRSSKRKRLDTEDSESPSEDAESGSAAMLAR
KPPQKMTTARRSRAAEVHNLSERRRRDRINEKMRALQELIPHCNKTDKASMLDEAIEYLKSLQLQVQMMWMGSAGIAAPP
AVMFPGVHQYLPRMGVGMGAAAAAALPSMPRLPFMAPQPVVPSAPVSVGPVPAYRGHMPAVGITEPYGHYIGVNHLQPAP
PPPQVQGVSYYPPPLGATAKAVQQAAELHHVPGPGGSIMPAGAAPGVLLPESAQGRGPGTVPCAPPFSSSASVFGLQMGA
LR
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map