TFome Information for pUT4140

TFome Name:pUT4140
Genbank Insert ID:KJ727070
Protein Name:ZmbHLH72
Species: Maize
TF Family: bHLH
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G170559
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGACACCCTGTTCGTGCTGGGCCAAGAGAGCAGGCTGAGGATCCTTCAGCAAGCCGCTGCTAGAGTTCCAGGCTGCGC
TTACCTTTGCGCTTGGGCTGCTGCTATCCCAAGCCAGCCACATAGGCCAGCTAGCAGCTCTAGCTCTGCTGCTGCTGCTA
CCACCTCTGCTAGGCTGCTGTGCTGCATCGATGCTTGGCTTTGCGACGGCGGCAGACCATCTCTTGGCGACGATGCTCAT
AGGCTGAGGGCCCTGTTCGATGCTTACAGGGGCTCTCTGTGCGCTCCAGTGTCTGGCTGCGTTCCAGGGTGGGCTTACAA
GGATGGCAGGGCCTACATGGAGCTGCCAGCTCATGATCTGACCACCAGCGCTTCTCTGCCAGTGCAGCAGCAGTTCTACC
AAGAGGCCGGCATCCAGATGGCCGCTTTCATGGGCTGCGAGTCTGGCGAGGTTGAGATCGGCATGTACACCGCTCCAACC
AGCGGCACCTCTCCAATGTCTCTCGAGAGCACCCTGCAGCAGGTTTTCAGCGAGGACTTCTTCCAGCAGAGCCTGCTCGA
GGAGCTGCTGCAGCTTCCACCAACCAGGCCAAGCAGCCCATCTGTGTCTGTTGGCTCTCCAGCTGCTGACGCTCTGACCT
CTCTGCCAAGGACCATGGCTGCTACCCCAACCCCAAGCTCATCTGTTGAGAGAAGGGCCCCACCAGTGCCTCCGCCACCA
CCACCACATGCTAGACCACCAGTGTTCCCAGTGCCATTCGCTAGACATGGCCCAGGCCATGTGAGGTTCCCATCTGCTGA
CACCGACGACGCTGCTATGGCCCAGGCTATGATCGACGTGATCAGCGGCGCTAGCTCTAGCAGCGCACTTCCAACCCCAC
CATCTACCGCTACTGCTCCTCCACCTCCAGGCAAGCACCATAGAGCTAGAAGGCGCAGAGGCGCCGCTACTGCTTTCAGG
GCTTACAACGCTGCTCTGGCTCCAAGGGCTCCATGGCGCCCACCAGGCGTTCCAGGCCAAAGGATGATCAAGATGGGCAT
CAGCATCCTGAGGCGCATGCACATGCTGAGGTTCTCAAGAGAGCGCACCGGCACCACTATGGCTCAAAGAGGCCAAGAGG
GCGAGGACGACCTTTCTAGCCCAGCTGTGCCAACCAGCAGCCAGCTGAACCACATGATCTCTGAGAGGCGCAGGCGCGAG
AGGCTGAACGAGTCTTTCGAGGCTCTTAGGGGCCTGCTGCCACCAGGCTCTAAGAAGGACAAGGCCACCGTTCTGGCCAA
GACCCTGGACTACATGAGCATCCTGGTGGCCCAGATCGCTGACCTCGAGGCTAAGAATCGCAGCCTCGAGTCTAGGGCTC
AGCACCACCATAGGCATGCTAACGGCGGCTCTAATGGCGGCAGGGTGGTGGTTCTTCAGGGCCTGATGTCTGGCACCAGC
AGCTCTGAGAGGGTGCAGGTTCACGTTACAGCTGGCGCTGGGGATGTGGGCACTTCTGCTTCATCTCCATCTGCCGGCCC
TCCACCACCAGCTTCTCGCGAGGTTGAGGAGACTGTGAGGGTTCACGCTAGGGCCGCTAGGTCTGATGTGGCTGAGCTTG
TGGCTAGGGCCCTGGCTGCTATTAAGGGCATGGGCAGGTTCACCGTGGTGGCTGTTGATGCTGGCAGGTCATCTGATGGC
GGCGGCGATTTCGCTCAGGCTACCTTCACTCTGAGGGCCACCTCTTGCAAGGAGCATTTCACCAAGCCACCAGACGCCGC
CCTGCTTGATGTTGAGGGCGTTGTGTGGATCGACTTCGCCCACGGCAACACCAATGCTCCATGCGCTCAGGACACCTTCA
GCAGGGAT
TFome clones do not contain stop codon at 3'end.
Translation: MDTLFVLGQESRLRILQQAAARVPGCAYLCAWAAAIPSQPHRPASSSSSAAAATTSARLLCCIDAWLCDGGRPSLGDDAH
RLRALFDAYRGSLCAPVSGCVPGWAYKDGRAYMELPAHDLTTSASLPVQQQFYQEAGIQMAAFMGCESGEVEIGMYTAPT
SGTSPMSLESTLQQVFSEDFFQQSLLEELLQLPPTRPSSPSVSVGSPAADALTSLPRTMAATPTPSSSVERRAPPVPPPP
PPHARPPVFPVPFARHGPGHVRFPSADTDDAAMAQAMIDVISGASSSSALPTPPSTATAPPPPGKHHRARRRRGAATAFR
AYNAALAPRAPWRPPGVPGQRMIKMGISILRRMHMLRFSRERTGTTMAQRGQEGEDDLSSPAVPTSSQLNHMISERRRRE
RLNESFEALRGLLPPGSKKDKATVLAKTLDYMSILVAQIADLEAKNRSLESRAQHHHRHANGGSNGGRVVVLQGLMSGTS
SSERVQVHVTAGAGDVGTSASSPSAGPPPPASREVEETVRVHARAARSDVAELVARALAAIKGMGRFTVVAVDAGRSSDG
GGDFAQATFTLRATSCKEHFTKPPDAALLDVEGVVWIDFAHGNTNAPCAQDTFSRD
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map