TFome Information for pUT4176

TFome Name:pUT4176
Genbank Insert ID:KJ727082
Protein Name:ZmbHLH62
Species: Maize
TF Family: bHLH
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G333582
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGCCATGCCGCCACCAACTCCAGCTGCTACCACTGCTTCTTGCGCTGCTACTCTGCCAGCCGCTGAGATCCAGGACGC
TTTCCTGAGCATCGACATGCACACCAGCATGCAGATCAGCATGAGGATGAACATGGACGACGAGGCCGCCGTGGTGTACC
ACATGTCATCTAATGGCGGCGCTACCGCCATCGATATGCACGACCCAATCGTGGACGGCCTGCTGGATGATCCACATCAT
GGCGCTGGCGGCAGAGCTTGCTCTAGCTTCCCAAGCAGCAGCAGCTGCAGCAGCCTGTCTCTTCCAGCTGCTAGCGCTTC
TCTGAGCTGCTCTCCAGACGACCCAGACAGCTCAAGCGCTCACCATATCCTGGCAGCTCCAGCTGCTGGCGGCGGCAATC
AACAATACCCAGAGGTGTCCAGCCACGTGCCACTGCTTCCGCCTCCGCCACCACCACCAGTTGTGCCATCTTACGACCAC
CACCATCAGTACACCGACCTGCACGCTCCAGCTCCAGCTAAGCCAACTGGCGCCTTCAAGCACTACGCTAGGCATCTGGG
CCCAAAGAGGCCACCAAAGCCAGGCGCTTGCGGCCAGAGGATGTTCAAGACCGCTATGAGCGTGCTGAGCAAGATGCACG
TGGCCGCTAGGTACAGCCAGCAGCAGTACTACTACCAGGCTGCTGCTGCCGCTGAGGCTGCTCAGCCACCACCATCTGTT
AACCAGCTGCAGCACATGTTCAGCGAGAGGAAGAGGCGCGAGAAGCTGAACGACAGCTTCCACGCTCTGAAGGCCGCACT
TCCACCAGGCGCTAAGAAGGACAAGACCAGCATCCTGATCAGGGCCAGGGACTACGTGAGAAGCCTTGAGGCTAAGGTGG
CCGAGCTTGAGGAGAAGAACAAGAGCCTCGAGAGCAGGCTGACCAGAAGGACTGACGGCAGCTCTGACGACGACGATAAT
GGCTCTGGCGAGACTACCAAGGTCGAGGTCGAGATCACCAGGACCGCTAACGAGGAGCTGTGCACCGTGAAGATCGAGGT
GAGAAGCCCACCAGCCGCTGCTGCTTCTCCATGCAACAGGACTGATGTGGTGGTGAGGACCCTGCAGTGCCTGAAGGAGC
AAATTGGCGACGGCGTGAGCCTGGTGGCTATGTCTACTTCTGGCGGCGCTGAGCCAGCTGCTACTGGCGTTAAGAACGCC
TTCCCAAGGGCCGTGCTGACCATGCAGATTAAGTCTCCAGGCACCGACTGGGAGGAGCAGCCAGTGAAGGATGCTGTGGC
TAAGGTGGTGGCCGATGCTCTGACCACCACCACTACTCCACCTCTGCCACCAGCTGCTGCTACTACTGTTCCACCAGCAG
CTTCTCCGGGCCTGCTGATCAACGACAGCAGCAGCGCTAACAACAGCCTGACCATCAGGCCGGCCATCATGAAG
TFome clones do not contain stop codon at 3'end.
Translation: MAMPPPTPAATTASCAATLPAAEIQDAFLSIDMHTSMQISMRMNMDDEAAVVYHMSSNGGATAIDMHDPIVDGLLDDPHH
GAGGRACSSFPSSSSCSSLSLPAASASLSCSPDDPDSSSAHHILAAPAAGGGNQQYPEVSSHVPLLPPPPPPPVVPSYDH
HHQYTDLHAPAPAKPTGAFKHYARHLGPKRPPKPGACGQRMFKTAMSVLSKMHVAARYSQQQYYYQAAAAAEAAQPPPSV
NQLQHMFSERKRREKLNDSFHALKAALPPGAKKDKTSILIRARDYVRSLEAKVAELEEKNKSLESRLTRRTDGSSDDDDN
GSGETTKVEVEITRTANEELCTVKIEVRSPPAAAASPCNRTDVVVRTLQCLKEQIGDGVSLVAMSTSGGAEPAATGVKNA
FPRAVLTMQIKSPGTDWEEQPVKDAVAKVVADALTTTTTPPLPPAAATTVPPAASPGLLINDSSSANNSLTIRPAIMK
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map