TFome Information for pUT4275

TFome Name:pUT4275
Genbank Insert ID:KJ727115
Protein Name:ZmARID1
Species: Maize
TF Family: ARID
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G365731
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGCTGGCGCCCCAGATCCTTCTATGGCTTCTGCTCTCGAGGCCCAGAGCAAGAGCATGGAGGCTCAGTCTAGGACCCT
GGCTACCCAGTCTCAGCTGCTTCAGCAGCTGTGCGACAGGCTCGAGTCTCAGGATCATAGGTGGTCCCAGATGGAGGACG
CCGTGAAGATGAACACCACCAACATCAGCAAGCTGCAAGAGAAGCTGGGCGACGGCGAGATCGAGGCTTTCAGGACTGAT
CTGACTCGCAGCCTGGGCGACAGGGTTGAGGCTCAGATTGCTCAGCTTGAGGCTGCTACTCTGGGCAGGGTTGACGGCCT
GGTGACCGAGACTTCTAACAGGGTGGCCGTGCTCGAGAACGCCACCTCTGCTTTCGAGTCTTGGAGGCCAAGGGTCGAGC
ACAGCGTTGACGGCATTAGGAACAGCGTGGACACCCTGAGGTCCGACCTGGCTAGGCTGACTCAGTTCCTCGAGAGGAAC
AACAACACCACCGGCGATCACCACGGCCAGGATTCTATCCTGGGCCCATACGTTCCAGCTGGCGAGCACACTTCTGCTAG
CCCAACTTGCACTGGCAGGCCAGATGGCCATGGCACCGCTACTACTAATCGCGAGTACGGCTTCGGCAAGCCGCACCCAA
TTCAGCTGCCAATCAACGGCCAGAAGTTCAAGAACAAGGAGCAGATCCACAGCCCGATCCCGACCCAGCATACAAAGATG
GCTGGCAACCCAGCCCCACCGCTGCCACTTAAGTCAAGCTCAAGCGCCCCAGCCTTCAAGAGCAACGCTTCTGGCTCATC
TAGGGGCCACGAGCCAGCTAAGGCTAGCTCTACTTCTCCAGCCGCTGCTGAGCTGCCAGTGCCAATCCCATCTGATAGCC
TGGTGGCTGTGCTTGGCGTTGTGGGCGAGCTTTCTGCTAGGGGCCTTCTGCAGGGCTTCACCATTCCAGAGGCTGGCGGC
CTTACTGACGAGGGCGCTTCTGCTGTTTTCGACGGGATGCTGACCGCCTTCCTTGCTGAGCATGGCGTTGACCCAGCTGC
CGCTTCTTGCCTTCCAATCCCACCACCACCACTTGGCGACGGCAAGAAGGTTGAGCTGGTCAGCCTGTTCCTGGCCGTTA
GAGCTAGAGGCGGCTTCAGCGCTGTTGAGTCTTGGGCTGCTGTGGCTGAGGCTGTGGGCCTTGATCCAACCGCTGATACC
GCTGTGAAGCTGCTGTACGGCAAGTACCTGGCTCTCCTCGAGCAGAGCTGCGACAAGCCACTGAAGCAGGACCACATGGT
GGTGAGCAGCGGCAACGCTGATGTGCTGATGGGCTCTAAGAAGGAGCGGTTCCTGAGCCCGATCAAGTGCCTGACTTCTA
CCGCTGGCAGCGCCTCTGCTCACCTTAAGAGAAAGCGCGAGACTCTGGTGGGCATGCTGGATTGGGTGCACCTGGTGGCT
AAGAACCCAGGCAAGCAGGGCAAGAATAGGGCCGCTGGCCATAAGCAGGCTGTGGTGGTTGAGCTTAGGCGCCAGATGTT
CGCCGACAAGGATTGCCTTGCTCGCGAGGAGCTGGTCAAGACCCTGAACTGGGTGAGGCTGGTGGCAAAGTCTGCTGGCC
ATCCAGGCATCATCGGCATGAACAACAGGCCAGACCACCTGGCCACCGCTGTTGAGTTGAGGTCTGCTATGAGCCTCGTG
GGCATGCTCGAGTGGGTTAGGCTGGTTGCTACCAGCCCAGCTGAGCCAGTTGTGATGGAGGGCAACAGCCTGAGCTACAG
GTCTACCGCTCTGCTGTTCCGCGAGCAGAAGTTCGCCAACAAGAAGTGCAGCCGCTGGTCTGCTGCTAGCCCACAGTCTG
GCATCAGCATTAGGCCATGCGGCCAGGCTGATATCCCAGAGTGGACTGGCAAGAGGTCCAGCCCATACGACGATCCAAGG
ACCCTTCGCTTCCTGGGCGAGCCAATTCTGCTGCCAGGCTCTACCGAGCATCTGGACGGCGCTTCTATTGGCAAGGGCAG
GCAGGACGATTGCAACTGCCAATTCCCAGGCTCTATCGCCTGCATCAGGTTCCACGTGGCCGAGAAGAAGATCGAGCTGA
AGCACGAGCTGGGCAGCGCCTACTACGAGATGGGCTTCCATAGGATCGGCGAGGACCTGGCTCTGACCTGGACTAAGGAC
GAGGAGACTAAGTTCAACAGCACCGTGCAGGACAACATGCCGGCCAGCAAGAACAAGCTGTGGGACAAGCTGCTGGCCGT
GCTGCATGCTAAGGGCAAGGAGGGCCTGGTCAGCTACTACCACAACGTGTTCCAGGTGTGGCGCAGGGCCTACCAGAATA
GGCTTGCTGGCAACGCTGACAGCGACGACGACTCTATTGAGCCGGGCTTCCTGTACTTCAGGCAAGGCGGCGATAGAAGC
AGGTCTAGGTCTGCCGCTAGCTCTAGAAAGAGGCGCAGGTCT
TFome clones do not contain stop codon at 3'end.
Translation: MAGAPDPSMASALEAQSKSMEAQSRTLATQSQLLQQLCDRLESQDHRWSQMEDAVKMNTTNISKLQEKLGDGEIEAFRTD
LTRSLGDRVEAQIAQLEAATLGRVDGLVTETSNRVAVLENATSAFESWRPRVEHSVDGIRNSVDTLRSDLARLTQFLERN
NNTTGDHHGQDSILGPYVPAGEHTSASPTCTGRPDGHGTATTNREYGFGKPHPIQLPINGQKFKNKEQIHSPIPTQHTKM
AGNPAPPLPLKSSSSAPAFKSNASGSSRGHEPAKASSTSPAAAELPVPIPSDSLVAVLGVVGELSARGLLQGFTIPEAGG
LTDEGASAVFDGMLTAFLAEHGVDPAAASCLPIPPPPLGDGKKVELVSLFLAVRARGGFSAVESWAAVAEAVGLDPTADT
AVKLLYGKYLALLEQSCDKPLKQDHMVVSSGNADVLMGSKKERFLSPIKCLTSTAGSASAHLKRKRETLVGMLDWVHLVA
KNPGKQGKNRAAGHKQAVVVELRRQMFADKDCLAREELVKTLNWVRLVAKSAGHPGIIGMNNRPDHLATAVELRSAMSLV
GMLEWVRLVATSPAEPVVMEGNSLSYRSTALLFREQKFANKKCSRWSAASPQSGISIRPCGQADIPEWTGKRSSPYDDPR
TLRFLGEPILLPGSTEHLDGASIGKGRQDDCNCQFPGSIACIRFHVAEKKIELKHELGSAYYEMGFHRIGEDLALTWTKD
EETKFNSTVQDNMPASKNKLWDKLLAVLHAKGKEGLVSYYHNVFQVWRRAYQNRLAGNADSDDDSIEPGFLYFRQGGDRS
RSRSAASSRKRRRS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map