TFome Information for pUT4686

TFome Name:pUT4686
Genbank Insert ID:KJ727252
Protein Name:ZmARF19
Species: Maize
TF Family: ARF
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: AC207656.3_FG002
Transcript: T002
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGAACGCGAGAGAGAATCTGATCGCTGCCTGGATCCACAGCTCTGGCATGCTTGTGCTGGCGGCATGGTTCAAATGCC
AGCTGTGCACTCCAAGGTGTACTACTTCCCACAAGGCCACGCTGAACATGCTCAGGGCCCAGTTGATCTTCCAGCTGGTA
GAGTGCCAGCTCTCGTTTTGTGTAGAGTGGCTGCTGTGCGCTTCATGGCTGACCCAGATACTGATGAGGTGTTCGCCAAG
ATCAGGCTGGCTCCAGTTAGGCCAAATGAGCCAGGCTACGCTGATGATGCTATTGGTGCAGCTGCTGCTTCTGGCGCTCA
AGAGGATAAGCCAGCTAGCTTCGCTAAGACCCTCACCCAGTCTGACGCTAACAATGGCGGCGGTTTCTCTGTGCCAAGGT
ACTGCGCTGAGACTATCTTCCCAAGGCTGGACTACAGCGCTGATCCACCAGTGCAGACTGTGCTCGCTAAGGATGTTCAT
GGCGTGGTGTGGAAGTTCAGGCACATCTATAGAGGCACCCCAAGGCGCCATCTGCTGACTACTGGTTGGTCTACCTTCGT
GAATCAGAAGAAGCTGGTTGCCGGCGACAGCATCGTGTTCATGAGAACTGAGAACGGCGATCTGTGCGTTGGCATTAGGC
GCGCTAAGAAGGGTGGTATTGGCGGCCCAGAGTTCATGCACCACCATCATCAACAACCGCCACCACCACAAGGTGGTGGT
TACGCTGGCTTCTCTATGTTCCTTAGGGGCGAAGAGGATGGCGGCAAGATGATGGCTGCCGCTGCTACTAGAGGTAAGGC
TGCTAGAGTTAGGGTGAGGCCAGAGGAAGTTGTTGAGGCCGCTAACTTGGCTGTTTCCGGCCAACCATTCGAGGTGGTCT
ACTACCCAAGGGCTAGCACTCCTGAGTTCTGCGTTAAGGCTGGTGCTGTGAGAGCTGCTATGAGGACTCAATGGTGCCCA
GGCATGAGGTTCAAGATGGCCTTCGAGACTGAGGACAGCAGCAGGATCTCTTGGTTTATGGGCACCGTGAGCGCTGTGCA
TGTTGCCGATCCAATTAGGTGGCCAAACTCTCCTTGGAGGCTGCTGCAAGTTGCTTGGGACGAGCCAGATCTGCTGCAGA
ACGTTAAGAGGGTGAGCCCATGGCTGGTTGAGCTGGTGTCTAACATGCCAGTGATCCATCACCTGACCGCTACCCCATTT
TCTCCGCCAAGAAAGAAGCTCTGCGTCCCGCTTTATCCTGAGCTGCCACTTGAGGGCCAATTCCCAGCTCCAATGTTCCA
CGGTTCTCCATTGCTTGGTAGAGGCGGTGCTGGTGGTCCAATGTGCTATTTCCCAGATGGTACTCCAGCCGGCATTCAAG
GTGCTAGGCATGCTCAATTCGGCATCAGCCTGTCTGACCTGCACCTGAACAAGCTTCAGCCAGGCCTTTCTCCACATGGC
CTGCATAGGCAACTTGACCACGGCGTGCAAGTGCAGGTTCAGCAACCTAGAATTGCCGCTGGCCTTATTGTGGGCGGTCA
TCCAGCTGCTAGGGACGATGTTTCTTGCCTGCTTACCATCGGCACCCCGAAGTCTAAGAAGCCACCAAGCGACGTCAAGA
AGGCTTCTACTGCAGCCGCTCCACAACTGATGCTTTTCGGCAAGGCTATCCTGACCGAGCAGCAAATTTCACTGGGCGGT
GGCAATGTGGTTCCAGCCCTTGCAAAGAAGTCCCCATCTGACGACGATGACGATGTGGCTGAGAGGACCGTGTCCAACTC
TGATGTTTCATCCCCAGGCAGGTCTAACCAGGACGGTACATCATCTGGTGGGGGACCTGCAGCTAGAGCTTGTTGGCAAG
AGGAAGAGTGCAACAACAGGGCTGCTGGCTCTGAGGATGATCTGCTTGGCCATTGCAAGGTTTTCATGCAGTCTGAGGAC
GTGGGCAGGACCCTGGATCTTTCTGCTGTTGCTAGCTACGAGGAACTGTACCAGAGGCTGGCAGATATGTTCGGCGTTGA
CAAGGCTGAGCTGACCAGCCACGTTTTCTACAGGGATGACGCTAGCGGCGCTCTTAAGCACCCTGGTGATGAGCCATTCA
GCGAGTTCACTAAGACTGCTAGGCGCCTGACCATCCTCACCGATGAGTCATCTGATAGCCTCGCTAGG
TFome clones do not contain stop codon at 3'end.
Translation: MERERESDRCLDPQLWHACAGGMVQMPAVHSKVYYFPQGHAEHAQGPVDLPAGRVPALVLCRVAAVRFMADPDTDEVFAK
IRLAPVRPNEPGYADDAIGAAAASGAQEDKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSADPPVQTVLAKDVH
GVVWKFRHIYRGTPRRHLLTTGWSTFVNQKKLVAGDSIVFMRTENGDLCVGIRRAKKGGIGGPEFMHHHHQQPPPPQGGG
YAGFSMFLRGEEDGGKMMAAAATRGKAARVRVRPEEVVEAANLAVSGQPFEVVYYPRASTPEFCVKAGAVRAAMRTQWCP
GMRFKMAFETEDSSRISWFMGTVSAVHVADPIRWPNSPWRLLQVAWDEPDLLQNVKRVSPWLVELVSNMPVIHHLTATPF
SPPRKKLCVPLYPELPLEGQFPAPMFHGSPLLGRGGAGGPMCYFPDGTPAGIQGARHAQFGISLSDLHLNKLQPGLSPHG
LHRQLDHGVQVQVQQPRIAAGLIVGGHPAARDDVSCLLTIGTPKSKKPPSDVKKASTAAAPQLMLFGKAILTEQQISLGG
GNVVPALAKKSPSDDDDDVAERTVSNSDVSSPGRSNQDGTSSGGGPAARACWQEEECNNRAAGSEDDLLGHCKVFMQSED
VGRTLDLSAVASYEELYQRLADMFGVDKAELTSHVFYRDDASGALKHPGDEPFSEFTKTARRLTILTDESSDSLAR
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map