TFome Information for pUT4687

TFome Name:pUT4687
Genbank Insert ID:KJ727253
Protein Name:ZmARR4
Species: Maize
TF Family: ARR-B
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G360523
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGACGCCGACACTTTCCCAGTTGGCCTTAGGGTTCTGGCTGTGGACGATGATAGGGTGTGCCTGAAGATCCTTGAGAG
GCAGCTGAAGTGCTGCAACTACAACACTACCGTGGTGACCGATGCTCAGACCGCTCTTGATATGCTTCGCGAGAGGAAGG
ACGGCAACCAGTTCGATCTGGTGATCAGCGACGTGGTGATGACCAAGATGGACGGCTTCAAGCTGCTCGAGCTTATCGGC
CTTGAGATGGACCTGCCAGTGATCATGCTGAGCGCTAACTCAGAGACTCAGACCATCATGAAGGGCATCAAGCACGGCGC
TTGCGACTACATGGTTAAGCCAGTTAGGCTTGAGCAGCTGAGGGGCATCTGGACTCATGTGGTGAAGAACAGCAAGATCG
ACCCGAGGAACAACATCGGCTCTGACTCTGATGACGTGGTCCAGAAGCTTCCATCTGGCGACGGTGATAAGGGTGAGAAG
GTTGGCGAGACTAGGCAGAAGAAGTACCCGAAGAAGAACAAGAAGACCGTCGACGTTGCCGACGAGGACAACGAAAAGAC
CAGCGCCCAAAAGAAGCAGAGGGTTCAATGGTGCGGCGAGCTGCACCAAAAGTTCGTTCAGGCTGTTAGGCAGATCGGCA
TCGACAGGGCTGTGCCAAAGAAGATTCTCGAGATCATGGACGTCGAGGGCCTGACCAGAGAGAATGTTGCTAGCCACCTC
CAGAAGTACAGGATCTACCTGAGGAAGTTGGGCGACGGCACTCTGAGATCTAGCTCTCCATTCGCTGACGAGACTGAGGC
TTTCAGGCGCAACATGAACGTGCCGAGCTTCATCTCTAGCAGCAGCTCCTCTAACCACTTCGCCAAGATGAACAGCTCCA
GCCTGATCGGCACTGAGACTTTGCTGCCAACCGAGAGCGTGTACATCATGAGCCCACAGAAGAACCTGGGCATCAGCCGC
TCTAACATGGAACCAGTGTCTCACGGCGTGAACCTGCCAAAGGATGTGATCCCAATGCCGGTGCAGGACATCTCTCGCTT
CATTAGCAGCGGCAAGAGCTACGCTCCAGTGTGGTCTGATGGACTGCTTGGTAGAAGCCAGTGCTTCCCTTCTGGCCCAT
CTGGTTCTAGCTTCGCTAACATCAGCAACAGCGTGGTGCTGAACGCCAGCAAGCCATTCTCTGTGGATATCTCTGGCCGC
TCTCTGGCTAACGGCTCTAATGATAGGCCACCGCTGACCAGCAACATGTGCTTCTCAAGCAGCCACTCCTGCAGCTCTTA
CGCTTCTATTCTGGGGGGCAAGATCCTCGGATCTTCTCGCAGAATCCCGTTCGAGGATATCGCCGATGGTGAGATGCTTG
CTCCAGGCAACCTTCCACTTCAGTCTCCACAGCTTGTGAAGCAGCCTTCATCTGCGGGCCTGTTCAACGAAGTGGCTAGG
GATGTTCATCAGTTCGCCGGCCTGAGCAACTCTTGGAAGGTGGCAGTTCCACCAAGGTTCTCTGATCTGGGCCATAACGT
TGGCACCTCTGAGGGACATTCACAGGGCAACATCTTCAAGATCAACAGGCTGTCAAGGCTGGCCAGGTCCTCTGCTCAGA
TCCCAACTTTCAGAAACGAATACCAGAAGAAGACCACGGGCATCATGGGCAAGGCTGTTCCAGTTGTTGGCTTCAGAGAG
CAGGTTGCCCCATTCAGCTTCGAGAACAACACCAGGTCTACCGTGACGCTCATCGGCAACAGTGCTCTTAGTAGCAGCTC
TAGCACTAGGCCAGGCCTGAACATCAACAACTCTGCCATGCTGACCCAGGTGTTGAATGGCGGTGGCGCTAACGATAACC
TGCATGGCGTTTCAACCGTGAACCAGCAGGCCGTTAACGACCAGGTGAACAACGAGTTCTTCATGGGGACCAACGAGGCC
CACAACGCTGAGTCAGATGATCTGGACGACTTCCTCGCCTACCTGGTCAACCAGGACTTCATTAACAACGGCGATTCCTT
CATCGACCGCGAC
TFome clones do not contain stop codon at 3'end.
Translation: MDADTFPVGLRVLAVDDDRVCLKILERQLKCCNYNTTVVTDAQTALDMLRERKDGNQFDLVISDVVMTKMDGFKLLELIG
LEMDLPVIMLSANSETQTIMKGIKHGACDYMVKPVRLEQLRGIWTHVVKNSKIDPRNNIGSDSDDVVQKLPSGDGDKGEK
VGETRQKKYPKKNKKTVDVADEDNEKTSAQKKQRVQWCGELHQKFVQAVRQIGIDRAVPKKILEIMDVEGLTRENVASHL
QKYRIYLRKLGDGTLRSSSPFADETEAFRRNMNVPSFISSSSSSNHFAKMNSSSLIGTETLLPTESVYIMSPQKNLGISR
SNMEPVSHGVNLPKDVIPMPVQDISRFISSGKSYAPVWSDGLLGRSQCFPSGPSGSSFANISNSVVLNASKPFSVDISGR
SLANGSNDRPPLTSNMCFSSSHSCSSYASILGGKILGSSRRIPFEDIADGEMLAPGNLPLQSPQLVKQPSSAGLFNEVAR
DVHQFAGLSNSWKVAVPPRFSDLGHNVGTSEGHSQGNIFKINRLSRLARSSAQIPTFRNEYQKKTTGIMGKAVPVVGFRE
QVAPFSFENNTRSTVTLIGNSALSSSSSTRPGLNINNSAMLTQVLNGGGANDNLHGVSTVNQQAVNDQVNNEFFMGTNEA
HNAESDDLDDFLAYLVNQDFINNGDSFIDRD
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map