TFome Information for pUT4693

TFome Name:pUT4693
Genbank Insert ID:KJ727259
Protein Name:ZmbHLH99
Species: Maize
TF Family: bHLH
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G076636
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGTCATGAAGATGGAACACGAGGACAACGGCGCTATCGGTGGCACTGATGGTACTTGGACTGAGGACGATAGGGCTCT
TGGCGCTGCTGTTCTTGGTGCTGATGCTTTCGCTTACCTGACTAAGGGCGGTGGCGCTATTTCTGAGGGCCTGGTTGCTA
CATCTCTGCCAGGCGATCTGCAGAACAAGCTGCAAGAGCTGGTTGAGTCTGAGAGCCCAGGCACCTCTTGGAACTACGCT
ATCTTCTGGCAGCTGAGCAGGACCAAGTCTGGCGATTTGGTTCTTGGCTGGGGTGATGGTTGTTGCAGAGAACCTAGAGA
TGGCGAGTTGGGTGCAGCTGCTTCTGCTGGTTCTGAGGACTCTAAGCAGAGGATGAGGAAGAGGGCTCTCCAGAGGCTGC
ATATCGCTTTCGGTGTGGCTGACGAAGAGGACTACTCTCCAGGCATCGATCAGGTGACCGATACCGAGATGTTCTTCCTG
GCCAGCATGTACTTCGCCTTCCCAAGGCATGCTGGTGGTCCAGGTCAAGCTTTTGCTGCTGGCATCCCAATCTGGGTGCC
AAACTCTGAGAGAAAGGTGGTGCCAGCCAACTACTGCTACAGGGGCTTCCTTGCTAATGCCGCTGGCTTCAGGACTATCG
TGCTTGTGCCATTCGAGTCAGGCGTTCTCGAGCTTGGCTCTACCCAGCACATTGCTGAGTCATCTGGCACCGTTCAGACT
GTGAGATCTGTGTTCGCTGGCACGAGCGGCAACAAGTCAGCTGTTCAAAGACATGAGGCCGAGAGGTCACCAGGCCTCGC
TAAGATTTTCGGCAAGGATCTGAACCTGGGCAGGCCATCTGTTGGCCTTGCTGTTGGTGTGAGCAACTCCAAGGTTGACG
AGAGAACTTGGGAGCAGAGATCTGCTGTGGGCGGCACTTCTTTGCTTCCATCAGTTCAGAAGGGCCTGCAGAATTTCTCT
TGGTCACAGGCTAGGGGCCTGAACAGCCACCAACAAAAGTTCGGCAACGGCGTGCTGATCGTGTCTAACAATGAGGGCGC
CCATAGGAACAATGGCGCTGTGGATTCTCCAAGCGCTGCTCAGTTCCAACTGCAGAAGGCTCCACAGCTCCAAAAGCTGA
GCGTGGTGCAAAAGACCCCACAGCTGGTTAATCAGCAGCCGATGCAGGCTCAAGTGCCAAGGCAGATCGATTTCTCTGCT
GGCTCTAGCTCAAAGCCAGGCGTGTTGGTTACTAGGGCTGGCGTTTTGGATGGTGAGTCTGCTGAAGTTGACGGCCTGTG
TAAGGAAGAAGGCCCGCCACCAGTTATGGAAGATAGAAGGCCAAGAAAGAGGGGCCGCAAGCCAGCTAATGGTAGAGAGG
AACCACTGAACCACGTCGAGGCTGAAAGACAAAGGCGCGAGAAGCTGAACCAGAGGTTCTACGCTCTTAGGGCCGTGGTC
CCAAACATCAGCAAGATGGACAAGGCTTCTCTCCTGGGCGACGCCATCACCTACATCACCGACCTTCAAAAGAAGCTCAA
GGAAATGGAAACCGAGCGCGAGAGGCTTCTTGAGAGCGGTATGGTTGATCCAAGGGAAAGAGCCCCAAGGCCAGAGGTGG
ACATCCAGGTTGTGCAAGATGAGGTGCTGGTGAGGGTGATGAGCCCGATGGAAAACCACCCAGTGAAGAAGGTGTTCCAG
GCCTTCGAAGAGGCTGAGGTTAGGGTTGGCGAGTCTAAGGTGACCAGCAACAACAACGGCACCGCCGTGCATAGCTTCAT
CATTAAGTGTCCGGGCACCGAGCAGCAGACCAGGGAAAAGGTTATCGCTGCTATGAGCAGGGCCATGAGCAGT
TFome clones do not contain stop codon at 3'end.
Translation: MVMKMEHEDNGAIGGTDGTWTEDDRALGAAVLGADAFAYLTKGGGAISEGLVATSLPGDLQNKLQELVESESPGTSWNYA
IFWQLSRTKSGDLVLGWGDGCCREPRDGELGAAASAGSEDSKQRMRKRALQRLHIAFGVADEEDYSPGIDQVTDTEMFFL
ASMYFAFPRHAGGPGQAFAAGIPIWVPNSERKVVPANYCYRGFLANAAGFRTIVLVPFESGVLELGSTQHIAESSGTVQT
VRSVFAGTSGNKSAVQRHEAERSPGLAKIFGKDLNLGRPSVGLAVGVSNSKVDERTWEQRSAVGGTSLLPSVQKGLQNFS
WSQARGLNSHQQKFGNGVLIVSNNEGAHRNNGAVDSPSAAQFQLQKAPQLQKLSVVQKTPQLVNQQPMQAQVPRQIDFSA
GSSSKPGVLVTRAGVLDGESAEVDGLCKEEGPPPVMEDRRPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRAVV
PNISKMDKASLLGDAITYITDLQKKLKEMETERERLLESGMVDPRERAPRPEVDIQVVQDEVLVRVMSPMENHPVKKVFQ
AFEEAEVRVGESKVTSNNNGTAVHSFIIKCPGTEQQTREKVIAAMSRAMSS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map