TFome Information for pUT4953

TFome Name:pUT4953
Genbank Insert ID:KJ727396
Protein Name:ZmbHLH2
Species: Maize
TF Family: bHLH
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G172795
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGCCCTGTCTGCTTGTCCAGCTCAAGAGGAACTTCTGCAACCTGCTGGTAGGCCACTGAGGAAGCAACTTGCAGCTGC
TGCTCGCAGCATCAACTGGTCCTACTCACTGTTCTGGTCCATCAGCTCTACCCAGAGGCCAAGGGTTCTGACTTGGACCG
ATGGCTTCTACAACGGCGAGGTTAAGACCCGCAAGATCAGCCACTCTGTTGAGCTGACCGCTGACCAGCTTCTGATGCAG
AGGTCTGAGCAGCTGAGAGAGCTTTACGAGGCTCTGCAATCTGGTGAATGCGATAGAAGGGCTGCTAGGCCAGTTGGCTC
ACTGTCTCCAGAGGATCTTGGCGATACCGAGTGGTACTACGTGATCTGTATGACCTACGCCTTCTTGCCAGGTCAGGGAC
TTCCTGGTAGAAGCTCTGCTTCTAACGAGCACGTGTGGCTGTGCAACGCTCATCTTGCTGGCTCTAAGGACTTCCCAAGG
GCTCTGTTGGCTAAGGTTCCCGAGGATCCAGACCTGATCAACAGGGCTACTGCCGCTTTCAGGGAACCACAGTGCCCAAT
CTACTCCGAGCAGCCAAGCTCTAACCCAAGCGCTGATGAGACTGGTGAGGCCGCTGATATCGCTGTTTTCGAGGGCCTGG
ATCACAACGCCATGGACATGGAAACTGCCGGCATTGCTGTGTTTGAAGGCCTCGACCATAACGCTATGGATATGGAGACA
GTTACCGCCGCTGCAGGCAGACATGGTACTGGTCAAGAACTTGGCGAGGCTGACAGCCCATCTAACGCTTCTCTCGAGCA
TATCACCAAGGGCATCGACGAGTTCTACAATCTGTGCGAAGAGATGGACGTGCAGCCACTTGAGGACGCCTGGATTATGG
ACGGCTCTAACTTCGAGGTGCCAAGCAGTGCACTTCCAGTGGACGGTTCTTCTGCTCCTGCTGATGGTTCTAGGGCCACC
TCTTTCGTTGCTTGGACTAGGTCTAGCCAGTCCTGCTCAGGTGAAGCTGCCGCTGTTCCAGTTATTGAGGAACCGCAGAA
GCTCCTGAAGAAGGCTGTTGCTGGCGGCGGTGCTTGGGCTAACACTAATTGCGGAGGTGGCGGCACTACCGTTACTGCAC
AAGAGAACGGCGCTAAGAACCACGTGATGTTGGAGAGAAAGAGGCGCGAGAAGCTGAACGAGATGTTCCTGGTGCTGAAG
TCTCTGGTGCCGAGCATCCATAAGGTGGACAAGGCTAGCATCCTGGCCGAGACTATCGCCTACCTTAAGGAACTGCAGCG
CAGGGTGCAAGAGCTTGAATCTAGAAGGCAAGGCGGTTCTGGCTGCGTGTCAAAGAAGGTTTGCGTTGGCAGCAACTCCA
AGCGCAAGTCACCAGAGTTTGCCGGGGGTGCTAAGGAACACCCTTGGGTTTTGCCAATGGATGGCACCAGCAACGTGACC
GTGACTGTGTCTGATAGGGATGTGCTGCTTGAGGTGCAGTGCCTGTGGGAAAAGCTGCTTATGACCAGGGTTTTCGACGC
CATCAAGAGCCTGCATCTGGACGCTCTGTCTGTGCAAGCTTCAGCTCTTGACGGCTTCATGAGGCTGAAGATCGGCGCTC
AATTCGCTGGATCTGGCGCTGTTGTTCCAGGCATGATCTCTCAGAGCCTTAGGAAGGCTATCGGCAAGAGG
TFome clones do not contain stop codon at 3'end.
Translation: MALSACPAQEELLQPAGRPLRKQLAAAARSINWSYSLFWSISSTQRPRVLTWTDGFYNGEVKTRKISHSVELTADQLLMQ
RSEQLRELYEALQSGECDRRAARPVGSLSPEDLGDTEWYYVICMTYAFLPGQGLPGRSSASNEHVWLCNAHLAGSKDFPR
ALLAKVPEDPDLINRATAAFREPQCPIYSEQPSSNPSADETGEAADIAVFEGLDHNAMDMETAGIAVFEGLDHNAMDMET
VTAAAGRHGTGQELGEADSPSNASLEHITKGIDEFYNLCEEMDVQPLEDAWIMDGSNFEVPSSALPVDGSSAPADGSRAT
SFVAWTRSSQSCSGEAAAVPVIEEPQKLLKKAVAGGGAWANTNCGGGGTTVTAQENGAKNHVMLERKRREKLNEMFLVLK
SLVPSIHKVDKASILAETIAYLKELQRRVQELESRRQGGSGCVSKKVCVGSNSKRKSPEFAGGAKEHPWVLPMDGTSNVT
VTVSDRDVLLEVQCLWEKLLMTRVFDAIKSLHLDALSVQASALDGFMRLKIGAQFAGSGAVVPGMISQSLRKAIGKR
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map