TFome Information for pUT5240

TFome Name:pUT5240
Genbank Insert ID:KJ727442
Protein Name:ZmARR5
Species: Maize
TF Family: ARR-B
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G133262
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGATGGAAAGGCTGTCTGCTCTGGTGCTGGACGGTGATCCAGCTTCTCTGAGCACCATCTCTGAGACTCTGGCCAAGTT
CAACTTCAGGGTGGTGAGCTTCCAGACCGCTGAGGCTGCTCTCGATTTCATTAAGTGCGGCGCTGCTAAGGCTGCTGACC
TGGATCTTGTGCTGGTTGAGGTGAACCTGAACAACATGGCTCCAGGCACCTCTGCTAACCCAGATCTGTTCCACCACATC
CTGAACGAGCTGCAGGTTCCACTGACCGCTATGTGCTCTAGGGACGATGAAGAGGCCCTGAGCAAGTGCATGAATCTGGG
CGCTTGCTTCCACCTCCTGAAGCCACTTAATAGGCGCAGCTTCAGCATCCTGTGGCATCAGGCTCTCGAGCACAAGTCTA
AGAAGGCCGCTCCACAGAGGCCAAGCCTCAACGATTCTACCAAGAACAGGATGGTGTCCCCGAGCACCGAGAAGCCAAAG
GAAGTGCTTATCCTCGAGAGGAACAACCTGAGCGTGCCACAGTCTAACGGTTACGAGGAACCGAAGAAGCAGAACAGGGT
CACCTGGACCATCGAGCTTCACGAGAAGTTCCTCGAGGCTGTTGAAGCTCTTGGCGGCAACAAGTCTGCTAGGCCAGAGA
AGATCCTGCACCTGATGAACGTTAAGGGCCTGACCGTTAAGCACATCGGCTCTCATCTTCAGAAGCACAGGCTGAGGAAC
CAGAACACTAAGCAGGGCCAGAGGCAAGAGAACGCCTCTAGGAAGCCAATTTCTAGGCAGGGCGAGGCTACCAGCGAGTC
TATTACTCCAAGGGTTGAGACTGACGGCGAGGTGTACCCATCTAGGCTGTGGACTAAGGTGAAGAAGCGCAGCGTTGACA
CCAGCGCTCTTAAGACCGGAATGCATACTTGCACCAGCGGCAACGATAACGAGGGCACTGAGTCTGTGTGGGACAGGTAC
GAGAACAATCTCCAGGTGTTCGCGGAGAACAAGAAGCGGTGGATGTTCACTTCAGAGCATCAGTGGCCAAGGCGCAACAA
CATCGATACAACCGATGAGGGCGACCCAAACGAGGCTCCAGAAACAGCTGCTGGCGCTAGCATTGAGATCTTCGGCTCTA
GCAGCCTGATCTCTGATCAGGTGGGCGAGAAGAATAGGGTCGAGTCTGACAGGGACAACAACACCGTGTGGTCTACCGAT
CTGCTTGAGGGCTACGGCGCTTCTATCGAGTCTAGCGGTTCTAGCTCTCTGCTGGCTAACGACCAAGTGGGTGAGAACAA
CCATTCTGAGGCCGCTAGGGCCAATGACAGCATGGGCAACATCACCCTGCTGGAAGAGATTGCTTACGGCGGTGCTTACA
TTGGCACTTCCGGCTCTGGTTCTCTGCTTACTGGCGATCTCGTTAGGCAGAATGGATGCGCAGAGGCGACCAGGCATAAC
GATAATCTGTGGGGCGTGAACCTCCCACAAGGTTACGGTGCTTTCACCGAGATCAGCGGCAGCTCTAACATGCTGACTAG
CTACCAGGTCGGCGAAAACAACGGCACAGAGTTCGCTAGGGATAACGGCCACATGGAAAACATCTCACTGCTCGAGGTTT
ACGGCGCCTCCATTGAGAACGCTGGTTCATCCTCTATGCTCACCTCTGACCAGGTTGGCCAGTCAAACCTTACCGAGGCC
GACAGGATCTACGACACCATGGGCTCTATCAACTACTTGGAGGGCACCGTGGACCCAGAGTACATTGCTCCAATGGACTA
CTCTGTGGCTTGGGCTCATCTTGAAGGCAACGGTCCAGATCTCAGGTCCCCATCTGATGGTAGGGAAGAGGCTTACAGCT
TCTGGATGTCTCAGCTTGCTGGCCCACAGGACTACCAGTCTCTTGGTCCAGAGGGCTTCCTTCAAGTTGGCGACGACGCT
TACAATGACGCTTTGAGATCAGTGGGCCTGGTGAACGTGGACGAGCAAGCTACTCAAGGCTGGCAAGAGCAGCAGAGCCT
GTCTATGGAAGATCTGCTCCAGGTTGACGACGTGGCCTGGGATGATACCCTTATCTCTATGGGCCTGATCAACCTCCTCG
ACGAGCCAATGGCAGAGGAAGCTGCTATTGGCGGTTCTCAAGGCCAACAGTCACTCGCTCCGGAAGTTCTGCAAGCTGGC
GATAATGCCTACGGCGACGATCTTGGCAGCATCGAGATGGTTAACCTGCTGAACGAACCCATGACCCAAGAGGCCGCAAT
TGCTGACGCTCTCGTGGATGATCCAATGACCCTGATCGCTCAGGACGATGTTCCTTGGGCTTGGTCACCAGCTGTTCCAG
GCGATTACGGCATCGATTGGATTCAGGATCCAAACGCCGAGCTGTACGGT
TFome clones do not contain stop codon at 3'end.
Translation: MMERLSALVLDGDPASLSTISETLAKFNFRVVSFQTAEAALDFIKCGAAKAADLDLVLVEVNLNNMAPGTSANPDLFHHI
LNELQVPLTAMCSRDDEEALSKCMNLGACFHLLKPLNRRSFSILWHQALEHKSKKAAPQRPSLNDSTKNRMVSPSTEKPK
EVLILERNNLSVPQSNGYEEPKKQNRVTWTIELHEKFLEAVEALGGNKSARPEKILHLMNVKGLTVKHIGSHLQKHRLRN
QNTKQGQRQENASRKPISRQGEATSESITPRVETDGEVYPSRLWTKVKKRSVDTSALKTGMHTCTSGNDNEGTESVWDRY
ENNLQVFAENKKRWMFTSEHQWPRRNNIDTTDEGDPNEAPETAAGASIEIFGSSSLISDQVGEKNRVESDRDNNTVWSTD
LLEGYGASIESSGSSSLLANDQVGENNHSEAARANDSMGNITLLEEIAYGGAYIGTSGSGSLLTGDLVRQNGCAEATRHN
DNLWGVNLPQGYGAFTEISGSSNMLTSYQVGENNGTEFARDNGHMENISLLEVYGASIENAGSSSMLTSDQVGQSNLTEA
DRIYDTMGSINYLEGTVDPEYIAPMDYSVAWAHLEGNGPDLRSPSDGREEAYSFWMSQLAGPQDYQSLGPEGFLQVGDDA
YNDALRSVGLVNVDEQATQGWQEQQSLSMEDLLQVDDVAWDDTLISMGLINLLDEPMAEEAAIGGSQGQQSLAPEVLQAG
DNAYGDDLGSIEMVNLLNEPMTQEAAIADALVDDPMTLIAQDDVPWAWSPAVPGDYGIDWIQDPNAELYG
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map