TFome Information for pUT5308

TFome Name:pUT5308
Genbank Insert ID:KJ727459
Protein Name:ZmABI31
Species: Maize
TF Family: ABI3-VP1
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G177244
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGCAGCCAGCTACCGATACTGAAGTTGTTGGCGGCGCTGTGAAGAAGGAACCGGAAGAGATCGTGGTTGAGGTGGACGG
TGATGGTGATGAAGAGGCCGAGAAGGTTGTGAAGAGAAGAAGAAGGCGCCGCAAGAAGACCACCGCTTGGGATCCACATA
AGAAGAGGGCTTGCGTCGACTGCACTAAGAGGTGCGCTAGGATTCATGGCCAACCAGCTTCTCCAAGCGCTCTGGCTTCT
AGCTCATCTAACGCTAGGCCAGTGCCAGCTGTGCCGTCATTCTTCAAGGTGATGCTGGGCTACTTCAGCGAGGACATGGA
TATCCCACCACCGTTCGCTAAGACCATCTGGGATCTTGCTGGCTCCAACGTGTTCCTTGAGGATGCTTTCGGTCTGAGGT
GGCGCGTTAGACTGTGTCTTAGAGATGGCGTGCTGTCTTTCGGCCACGGCTGGAAGAACTTCGTGCTGGATCATGCTGTT
AGCTGCGGCGAGTTCCTGGTGTTCAGACAGATTGCTCGCAGCGTGTTCACCGTGCAGATGTTCGCTCCATCTGCTGTTGA
GAGGCTGCATCTGTGCGAGAGGAACAAGAGGCAGTCTAGGAAGAGGAAGCCAAGGCCACAGAGGCAGAGAACCTCAAGCC
CATCTATCCAGACCGTGAAGGCCACCAAGAACAGGGTGAAGAACAGCAACAAGAAGCGCCAGAGGACCGACGATCATCAG
AATGGTGTTAGGCCAAGGGGCCACAAGTCTAAGACTGCTGCTCAGGTGTGCGTGGACGACTCTGATGTTCCAGATCCTGC
TAGCGAGCCAAAGTGCCCAGATACTTCTGAAAGGCTGCCAGAGGCTGAGGTGACAGAGCCACAAGAGATTTCTGAACCTC
CAGCCGGTAGGCACGAGAGGGAAGCTCAAAAGGTTTTGGACGGCGAGGCTGAAGTGGCTGATGACTCTGCTATGCTCGAG
GAAACCGAGACTATCTACAACGCCAGGCTGACCGAGCATCAGGTTCCAGCTGCTAACGAGACTGAGCATGGCGAGTGCCT
CGCTAACTTCGACGCTTCTATCCCGCTGGCTATGATGGACCTGAACGAAGTGTCTATCGACGACATCTTCCTGAGCGCCG
ACACTTACGAGTTCGGCTCTGATATGTGCAACCCAGAGCCATTCTCTGTGGGCCTGAACATGGAAGGCACTACCACTGGC
CAGAACAGCGGCTTTTCTTGCTTGGAGGAACTGGACACCCCACAGAACCACCTTTCTTCTATGGGTGACGGCCACTGCTC
TCTGGTTCCAGAGGCCGGTCCATGCACTGAGAACAACAAGGAAAAGGCCGACGCTCTCGAGACTGGCGCTAGCTACTTTT
GCGACAGCAGCTTCCACGACATCGACATCAATGCCCTGCCAGCCAAGGAACCACCATCATTCGGTGAGGACTCTCCATCT
CCAAGAGCTGGCGCTGAGATGCATCCAAATGAGTGCGGTCTGTCTAGCTGCTCTCAGGACAGGGGCAACTCTAGCCTGCT
GATCATTAACAAGCAGGCCGCCCACAAGGAATCCGACTCATTCATGACCACCAAGCAGGACAAGCCACAGGGCGGTCAAT
GCTCTATGCAAGATGGTGTTGGCAGGCAGCACCCAACCGAGATCACTTCTAGGAATGCTAAGCCACGCGAGAGCCCAGAG
CTTACTAGAAACCCACTTGGCACCGGCACAGGCTCTGAGTCTACTCATTCTGGCACCTCTGAATCTGGCGGCCTTCTGGC
TCTTACCGAGAACATCACCAAGTTCTGCATTGCCGTTCCAGCCCCAGGCCAAACTTGGCTTGAAATTCCAGGCAGGCTGC
CGGTTATCCCGAGGACTAAGAAGCACGGTCGCAAGGTCGTGATGCTCAAGGATCCAAGGATGAGGCTGTGGCCAGTTCTG
TACCAATGCACCCCAAGGTTCAACGGCATCATCACTGGTTGGGTTGACGTGTGCAGAGAGAACAGGATCAAGGAAGGCGA
TACCTGCGAGTTCGAGCTGAGCGGCAATTCTGAGCTGTCATTCCAGCTTCAGGTGAGGGTGCCAAACACCCATCCAGAAT
GT
TFome clones do not contain stop codon at 3'end.
Translation: MQPATDTEVVGGAVKKEPEEIVVEVDGDGDEEAEKVVKRRRRRRKKTTAWDPHKKRACVDCTKRCARIHGQPASPSALAS
SSSNARPVPAVPSFFKVMLGYFSEDMDIPPPFAKTIWDLAGSNVFLEDAFGLRWRVRLCLRDGVLSFGHGWKNFVLDHAV
SCGEFLVFRQIARSVFTVQMFAPSAVERLHLCERNKRQSRKRKPRPQRQRTSSPSIQTVKATKNRVKNSNKKRQRTDDHQ
NGVRPRGHKSKTAAQVCVDDSDVPDPASEPKCPDTSERLPEAEVTEPQEISEPPAGRHEREAQKVLDGEAEVADDSAMLE
ETETIYNARLTEHQVPAANETEHGECLANFDASIPLAMMDLNEVSIDDIFLSADTYEFGSDMCNPEPFSVGLNMEGTTTG
QNSGFSCLEELDTPQNHLSSMGDGHCSLVPEAGPCTENNKEKADALETGASYFCDSSFHDIDINALPAKEPPSFGEDSPS
PRAGAEMHPNECGLSSCSQDRGNSSLLIINKQAAHKESDSFMTTKQDKPQGGQCSMQDGVGRQHPTEITSRNAKPRESPE
LTRNPLGTGTGSESTHSGTSESGGLLALTENITKFCIAVPAPGQTWLEIPGRLPVIPRTKKHGRKVVMLKDPRMRLWPVL
YQCTPRFNGIITGWVDVCRENRIKEGDTCEFELSGNSELSFQLQVRVPNTHPEC
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map