TFome Information for pUT5309

TFome Name:pUT5309
Genbank Insert ID:KJ727460
Protein Name:ZmABI39
Species: Maize
TF Family: ABI3-VP1
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G172621
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGCTATGGCTGCTGCAGCTGCCGCTGCTACTAAGAGATGCATGAATCCAGCTTGCGGCGCTCCAGCTTCTGTTCTTGG
TGCTGGTGGTGATTGGAGAAAGGGTTGGCCACTTAGGTCTGGCTGCTTCGCTCTGCTTTGCGATAAGTGCGGTCTTGCCT
ACGAGCAGTTCGTGTTCTGCGACATCTTCCACCAGAAGGAAAGCGGTTGGAGGGACTGCTCTTTCTGCGGCAAGAGACTT
CATTGCGGCTGCGTGGCCAGCAAGAACTCTTACGATCTGCTTGATTCTGGCGGCGTGCACTGCGTTACCTGCATGAAGAA
TAGCCTGGCTCAGTCTGCTTCTGGCCAGGTGGTGCCAAAGCTTTTCCCAAGCCCAATCAACCTGCGCTTCTTCGGCAAGT
CTGACGAGCTTCTCAGCTCCAGAAACTTCGAGCAGCCACCATCTCTGACCCTGGACTCTAGGAACGACGATATCGCCTTC
GTGAACAAGAGCAACCACCTGTTCATGGTCAAGGGTATCGAGGCTGGCCAGTCCAGCAACATCCTGAGGCAAAAGGAAAT
CGAGAACGGCAGCAGGCAGATCAAGTGGGAACAGCCAACTCTGAGCTTCGGCGATATGGGCAGGCCATTCCTTACTAGAT
CTCAGAGCGCCCTCGAGAGCCCACAATGTACTAGAAGGGACGACAACAAGGACCCGACCACCGATTCTACCAGCGAGTCA
TTCTCTGAGGCCTGCCTGTCTATGAGCCTGGGCATTGCTAACAACGTGAACAGGATGGAAGCCACCTCTACCGCTGAGAG
GCCAGTGCTGCTTTCTACCACTGCTATTGCTGAGGGCAGAGAGCTGGCTACCACTCTGTCTCCATTCCAGCATGCTCAGA
GGGCTAGGCATTTCCTGACTAGGCCACCAAGGATTGGTGAGGGTGCAGCTTTCGATCCAACCAGGGACATGTTTCCACAT
CTGAGGGTTGCAAGGCCTCCAGCTGATGGTAGGGGTAGGAATCAGCTGCTTCCAAGGTACTGGCCAAGGATCACCGATCA
AGAGCTTCAGCAGATCAGCGGCGACAGCAACTCTACTATCGTGCCGCTGTTCGAGAAGGTGCTGAGCGCTTCTGATGCTG
GTAGGATTGGCAGGCTGGTTCTGCCAAAGGCTTGCGCTGAGGCTTACTTCCCACCAATCTCTCAACCTGAGGGAAGGCCA
CTGACCATCCAAGATGCAAGAGGCAAGGAATGGCACTTCCAGTTCAGGTTCTGGCCGAACAACAACAGCAGGATGTACGT
GCTCGAGGGTGTGACTCCATGCATCCAGTCTCTTCAACTCCAGGCTGGCGATACCGTGACCTTCTCTAGAATTGATCCAG
GCGGCAAGCTCGTGATGGGTTTCAGAAAGGCTACCAACACCGTGAGCCTCCCAGACTCTCAGATCTCTGCTATCGCTAAC
GGCTCCCTGCTGTCCGAGACTCTGTTCTCTACTGCCAACGAGAACATCGGCGTGGTGTCTGGTTACCCAGGCTTCCTGCA
TTCTATCAAGGGCGCTGCCGATCTGCATCCGAACTCACTGTACGACCACCACATGAACCTGGTGGATGGTGACGTGAGCT
GGAACAAGGCTGACAAGTTTGGCTCTAGGCCAGACGAGGGCTCTCTTCAGTTTCTCCAGAAGAGGTCCCACAACATTGGC
AGCAAGTCTAGGCGCTTCCTGATCGACGCTGAGGACGCTATGGAACTGAAGCTGACTTGGGAGGAAGCCCAAGAGCTGCT
TAGACCAGCTCCAACTGCTAAGCCAACCGTGGTGATGATCGAGGACTACGAGTTCGAAGAGTACGACGAGCCACCAGTGT
TCGCTAAGCGCAGCATCTTCACTATCAGGGCTACTGGCGAGCAGGACCAGTGGATTCAGTGTGACGATTGCTCAAAGTGG
CGCAGGCTGCCACTGAACGTTATCGTGGCTCCAAAGTGGACCTGCACCGACAACTCTTGGGACAGCAAGTGCTGTAGCTG
TAGCGCCCCAGAGGAACTGACTCCAAGGGAACTTCAGTCTGTGCTCCAGCAGTACGAGGAAATGAGGCGGAGGAAGGGAT
CTTACGGCCTCAAGCTTAACGTGGCCGAGATGGATGCTTCTAGCCTGGACGCTTTTGCCACCGCTGCTGTTTTCGGTGAG
GTTGGCAATCAAGGCTCTGCCTCTGTGGCTACTACCACTAAGCACCCAAGACACAGGCCAGGGTGCACTTGCATTGTGTG
CATTCAACCACCAAGCGGCAAGGGCCCAAAGCACTCACCAGCTTGTACATGCAATGTCTGCATGACCGTTAGGCGCCGCT
TCAAGACTCTGATGATGAGAAAGAAGCAGCGCCAGAGCGAGAGGGAAGAAGCTGAAGCAGGCAAGAAGATCGCCTGGGTT
AACAGGGATGAGCCAGAGGGATCTAGCCTCTCTAGGTCACCACAGACTCTGGACTCCACTAGGGATAACTCTGACGTGAC
CGCTACCATGCTCGACAAGGTGAGCGACGTTAACAAGCAGGGCCATATCGACGTGGACCTCAACCTTCATCCAGCTGCCC
CAGCTGTTCAGGCTGCTCAGCAACAACCACGCCCAGTTTCTATGATGGGCCTTCTGGAAGTTGCCGGTAGGCCACTCGAC
AACTACATGAAGCAGAACGGCCTGACTTCTCTGGTGGGTGAGCAAGGTGGTGGTTCTTCTTCTACTGCTACCGTTCCTCC
ACCAGCTCTGGTTGAGTCTGAGGAAAGGACTTCTAACGAGGGCCTGGTGCCATCTGCTAGCGCTGAAAGGGAACCTAACG
CCATGGCTGTTGATGAGGCCGGCGAGAATCAGCAAGATAAGGCTGCTGACGACGACGATGACGCTGTTGCTGCTGCGGCT
ACT
TFome clones do not contain stop codon at 3'end.
Translation: MAMAAAAAAATKRCMNPACGAPASVLGAGGDWRKGWPLRSGCFALLCDKCGLAYEQFVFCDIFHQKESGWRDCSFCGKRL
HCGCVASKNSYDLLDSGGVHCVTCMKNSLAQSASGQVVPKLFPSPINLRFFGKSDELLSSRNFEQPPSLTLDSRNDDIAF
VNKSNHLFMVKGIEAGQSSNILRQKEIENGSRQIKWEQPTLSFGDMGRPFLTRSQSALESPQCTRRDDNKDPTTDSTSES
FSEACLSMSLGIANNVNRMEATSTAERPVLLSTTAIAEGRELATTLSPFQHAQRARHFLTRPPRIGEGAAFDPTRDMFPH
LRVARPPADGRGRNQLLPRYWPRITDQELQQISGDSNSTIVPLFEKVLSASDAGRIGRLVLPKACAEAYFPPISQPEGRP
LTIQDARGKEWHFQFRFWPNNNSRMYVLEGVTPCIQSLQLQAGDTVTFSRIDPGGKLVMGFRKATNTVSLPDSQISAIAN
GSLLSETLFSTANENIGVVSGYPGFLHSIKGAADLHPNSLYDHHMNLVDGDVSWNKADKFGSRPDEGSLQFLQKRSHNIG
SKSRRFLIDAEDAMELKLTWEEAQELLRPAPTAKPTVVMIEDYEFEEYDEPPVFAKRSIFTIRATGEQDQWIQCDDCSKW
RRLPLNVIVAPKWTCTDNSWDSKCCSCSAPEELTPRELQSVLQQYEEMRRRKGSYGLKLNVAEMDASSLDAFATAAVFGE
VGNQGSASVATTTKHPRHRPGCTCIVCIQPPSGKGPKHSPACTCNVCMTVRRRFKTLMMRKKQRQSEREEAEAGKKIAWV
NRDEPEGSSLSRSPQTLDSTRDNSDVTATMLDKVSDVNKQGHIDVDLNLHPAAPAVQAAQQQPRPVSMMGLLEVAGRPLD
NYMKQNGLTSLVGEQGGGSSSTATVPPPALVESEERTSNEGLVPSASAEREPNAMAVDEAGENQQDKAADDDDDAVAAAA
T
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map