TFome Information for pUT5362

TFome Name:pUT5362
Genbank Insert ID:KJ727503
Protein Name:ZmbZIP72
Species: Maize
TF Family: bZIP
Template: BT036137.1
Corresponding TF Gene: GRMZM2G030280
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: B476_F 3' Primer Name: B476_R
5' Primer Sequence: caccATGGCAGATGCCAGCCCTAG 3' Primer Sequence: TTCCTTGGGCCGAGCAA
5' Primer Tm (º C): 63.9 3' Primer Tm (º C): 57.4
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 1.25 l DMSO 100% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 13.5 l ultrapure water PCR program: 40 sec 98°C 35x : 15 sec 98°C 30 sec 60°C 40 sec 72°C 7 min 72°C leave at 10°C
Nucleotide Sequence: ATGGCAGATGCCAGCCCTAGAACAGAAACATCAACAGATGATACTGACGACAATAACGGGCTTGAACCAGGCAGAGGTGG
TCTTGTTGCTTCTGACTCCAGTGACAGATCCAAAGACAAACATGGAGATCAAAAGACATTGCGTCGCCTTGCTCAAAATC
GTGAGGCTGCAAGGAAGAGCCGACTAAGGAAGAAGGCATATGTTCAACAGTTGGAGAATAGCAGGCTAAAACTTACCCAA
CTAGAGCAAGAGCTGCAAAGAGCTCGTCAGCAGGGCATTTTTATATCTAGTTCAGTTGATCAGTCACACTCCATGAGTGG
AAATGGGGCATTGGCCTTTGATATGGAGTATGCGAGGTGGTTGGAAGAGCACAATCGGCAAATTAGTGAACTAAGGGCTG
GTGTCAGTGCTCATGCAAGTGATACTGATCTTCGAAGTGTTGTTGATAAGATCATGTCACACTATGATGAGATTTTTAGG
CTCAAAGGCAATGCAGCCAAGGCAGATGTTTTTCACGTCTTATCTGGCATGTGGAAGACACCGGCGGAGAGGTGTTTTTT
GTGGCTAGGAGGTTTCCGACCATCTGAGGTTTTAAAGCTTCTTTCGACACAACTTGAACCTCTCACCGAACAGCAGCTGT
CAGGGATAAGCAACCTTCAGCAATCTTCACAGCAGGCTGAGGATGCTCTTTCACAAGGAATGGAGGCCCTTCAGCAATCA
TTGGCCGAAACCTTGGCTGGGTCTCTTTCGTCTTCTGGATCAACAGGAAATGTGGCAAACTACATGGGTCAAATGGCCAT
GGCTATGGGAAAACTTGGAACGCTTGAGAATTTCCTTCGTCAGGCTGACAACCTGCGGCTGCAGACTCTACAACAGATGC
AAAGGATCCTGACCACTAGACAGTCAGCTCGGGCGCTACTTGTGATAAGTGATTACTCTTCACGTCTCCGTGCCCTAAGC
TCTCTCTGGCTTGCTCGGCCCAAGGAA
TFome clones do not contain stop codon at 3'end.
Translation: MADASPRTETSTDDTDDNNGLEPGRGGLVASDSSDRSKDKHGDQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTQ
LEQELQRARQQGIFISSSVDQSHSMSGNGALAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFR
LKGNAAKADVFHVLSGMWKTPAERCFLWLGGFRPSEVLKLLSTQLEPLTEQQLSGISNLQQSSQQAEDALSQGMEALQQS
LAETLAGSLSSSGSTGNVANYMGQMAMAMGKLGTLENFLRQADNLRLQTLQQMQRILTTRQSARALLVISDYSSRLRALS
SLWLARPKE
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map