TFome Information for pUT5363

TFome Name:pUT5363
Genbank Insert ID:KJ727504
Protein Name:ZmbZIP111
Species: Maize
TF Family: bZIP
Template: BT042103.1
Corresponding TF Gene: GRMZM2G073427
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: B490_F 3' Primer Name: B490_R
5' Primer Sequence: caccATGGCGGGTGGCGGCGCC 3' Primer Sequence: CCTATCGGCAAGAGCTTCAGGATCTGAGAATGTCGTTGA
5' Primer Tm (º C): 72.7 3' Primer Tm (º C): 65.8
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 1.25 l DMSO 100% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq 13.5 l ultrapure water PCR program: 40 sec 98°C 35x : 15 sec 98°C 8 sec 65°C 40 sec 72°C 7 min 72°C leave at 10°C
Nucleotide Sequence: ATGGCGGGTGGCGGCGCCGGCGACGACGACGTGGTGGTGGTGAGCTGCGGCGGCAGAGGCGGCGGCGACCCCGGCGCATA
CGCCGCGGTGCTCAAGAGGAAGCTCGACCTGTACTGCGCCGCCGTCGCCAAATCCATGGAAGCTAAATCACAAGAACATT
CGTTGGGCTACCCCAACTCACAAGCTTCAGATGCTTCGCAATTGATTTCTCAAGCTTCCTTTGATGGTGACATAGATGGG
GCAAGCCTAGTTACAAACTCTAATGTCATAGAAGATGATGATTTTCAAGGCAAGCCAACCAACAGTGGCACATCAAAGGA
ACTGTCAGACGATGATGGTGACCTTGAAGAGAATACTGACCCCGCTAACGCCAAGAAGATGAGGAGAATGGTGTCAAACC
GGGAATCGGCTAGGCGATCAAGAAAGAGGAAGCAAGCACACTTGACCGATCTTGAATCACAGGTTTCCAGGTTAACGTCT
GAGAATGCATCTTTGCTGAAGCGACTGGCTGATATGACTCAGAAATACAAGGACGCTTCCGTTGACAATAAAAATCTAAC
AGTTGATGTTGAGACTATGAGGAGAAAGGTGAACATAGCCGAGGAAGCTGTCAGGAGGCTAACTGGGATCACCCTCATGC
TACCGACCGCTTTTGAGAAGCCCACGAGCAGCGCGCCCCTGTCATCATGTCCATCTGATGCAGCTTCTGCTTCTGCTTCT
GCCGCCATTGAAGACAGCGTGAAGCATTTCCTCCAGGCGCCACTTGAAAACAGTGGGATCATCCCAAACGCAGCAGTTTT
TCAGACCAAGCCAGCTTCTCTGCACCGTGTTGCTAGCCTGGAGAACCTGCAGAAGAGGATCGTCGGGGATGCAATGTATT
CCGAGACCGCGTCAACGACATTCTCAGATCCTGAAGCTCTTGCCGATAGG
TFome clones do not contain stop codon at 3'end.
Translation: MAGGGAGDDDVVVVSCGGRGGGDPGAYAAVLKRKLDLYCAAVAKSMEAKSQEHSLGYPNSQASDASQLISQASFDGDIDG
ASLVTNSNVIEDDDFQGKPTNSGTSKELSDDDGDLEENTDPANAKKMRRMVSNRESARRSRKRKQAHLTDLESQVSRLTS
ENASLLKRLADMTQKYKDASVDNKNLTVDVETMRRKVNIAEEAVRRLTGITLMLPTAFEKPTSSAPLSSCPSDAASASAS
AAIEDSVKHFLQAPLENSGIIPNAAVFQTKPASLHRVASLENLQKRIVGDAMYSETASTTFSDPEALADR
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map