TFome Information for pUT5369

TFome Name:pUT5369
Genbank Insert ID:KJ727510
Protein Name:ZmbZIP126
Species: Maize
TF Family: bZIP
Template: gDNA
Corresponding TF Gene: AC186606.4_FG003
Transcript: T003
Vector: pENTR/SD/D-TOPO

5' Primer Name: SE0001_F 3' Primer Name: SE0001_R
5' Primer Sequence: caccATGTACCACCACCATTTCCAT 3' Primer Sequence: GCAATACGACTTGCATGGCTCG
5' Primer Tm (º C): 59.4 3' Primer Tm (º C): 59
PCR Condition: Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98°C 40 sec melt, 72°C 60 sec extend, 35 cycles; Step 3, 72°C 10 min extend 1 cycle; 1ul Phusion (Thermo Scientific), GC buffer (Thermo Scientific), MgCl2 3 mM final concentration,5% DMSO, template 50ng/ul final concentration.
Nucleotide Sequence: ATGTACCACCACCATTTCCATGGCGAGGTGGCTAGCCTCCGCTTCCTGTCCCCTCCGAACGTCTCGACGTCCTTCGCGGC
GCACCACCACATGAACATGGCACTGCCGCCTCCATCCTACTTCCAAGACGACGACGCTACCCTTGCAGCCATCGACGGCG
GTGACGCGGCCTGTTTTGATGAGCTTGACACCATCGTCGTCGTCCCGGAAGCAGATGCCGCAGCCGCAGCCCGCTGCCAC
CTCACCGGCAGCAACAATGGCGGCTTGTCGGACTCCGACCAGCTGCTGGACGCTGCTGTGATTATGGCGGCCGACGAGGA
GCGCCGGCGCCGGCGGATGGTGTCGAACCGGGAGTCGGCGCGGCGGTCGCGCATGCGCAAGCAGCGGCAGCTCAGCGAGC
TGTGGGCGCAGGTATCGCACCTCCGCGGCGCCAACCGCCGCCTCCTCGACGACCTCAACCGCGCGCTCAGGAGCTGCGCC
GACGCCCGCCGGGAGAGCGCCAGGCTGAGGGAAGAGAAGGCCGAGCTGACGAAGAAGCTCGAGCAGCTGCTGCAGGCGGA
GAAGGGCTCGTTATCGGAGGCGGCCGAGCCATGCAAGTCGTATTGC
TFome clones do not contain stop codon at 3'end.
Translation: MYHHHFHGEVASLRFLSPPNVSTSFAAHHHMNMALPPPSYFQDDDATLAAIDGGDAACFDELDTIVVVPEADAAAAARCH
LTGSNNGGLSDSDQLLDAAVIMAADEERRRRRMVSNRESARRSRMRKQRQLSELWAQVSHLRGANRRLLDDLNRALRSCA
DARRESARLREEKAELTKKLEQLLQAEKGSLSEAAEPCKSYC
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map