TFome Information for pUT5421

TFome Name:pUT5421
Genbank Insert ID:KJ727561
Protein Name:ZmbZIP107
Species: Maize
TF Family: bZIP
Template: BT038664.1
Corresponding TF Gene: GRMZM2G094352
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B536_F 3' Primer Name: B536_R
5' Primer Sequence: caccATGGGGATCTACGAGCGGCA 3' Primer Sequence: TGCGGGCTCCCTGGGGC
5' Primer Tm (º C): 64.8 3' Primer Tm (º C): 67.3
PCR Condition: PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGGGATCTACGAGCGGCAGCGCCACCTGGTGCCCACCGGCGTGTGGGGGGAACCGTTCCGGCTCGACGCCGACGCCGT
GGCCATGCCCCTGCCCCTGGCCGCGTTCCCCTCGGTCACCGTGGCCACGCCGGCGCCGCTCGACGTCGTCGAGCCGGAAG
CAGAGGAGATCAAGCTCGGCAAACGACTGTTGCAGGCGCAGCAGGACGACGTGCCGCGGATGCAAGAAGCGCCGCCGTCG
TCGGATAGCTTCAGCCACGACGATGACGACGCTAGGCCGAGAGACAAGGTTCAGAGACGGCTCGCGCAGAACAGAGAGGC
AGCTCGGAAGAGCCGGCTACGGAAGAAGGCGTACATACGGAACCTCGAGACGAGCCGCGTGAAGCTGGCGCAGCTGGAGC
AGGAGCTCATAATGGCCAGGCGCCAGCAGCACGGCGCGTACGGCGTGGGAGGAGGAGTCGCACCGCCGGCGGCGCCCGTG
GACCCGCGCGTCGCTGCGTTCGAGCTGGAGTACGCGCACTGGGTGGAGGAGCAGAGCAGGCAGGCGACGGAGCTGCGGGC
GGCGCTGCAGTCGCACGCGCCGGACGTCCAGCTGCGGGTCCTCGTCGACGCCGCGCTGGCGCACTACGGCGCGCTGTTCC
AGGCCAAGGCGAGGGCAGCCCGGTCCGACGCCTTCTTCGTGCTGTCCGGCGTGTGGCGGTCCCCCGCGGAGCGCTTCTTC
CTCTGGATCGCCGGCTTCCGCCCCTCCGACCTGCTCAAGGTGCTGGAGCCGCAGCTGAGCCCGCTCATGGACCACCAGGC
GTCCGAGGTGCGCAAGCTGCAGAACACGGCGCGGCAGCTGGAGGACGCGCTGTCGCAGGGCATGAGCAAGCTGCAGCAGA
CGCTGGTCGACACCCTCATGACCGTCGACGTCTCCCCGGACGGCGCCGGGGGCGGATACGCGGGGCAGCAGATGGCCTGC
GCCGTGGGCAAGCTCGCCGACCTCGTCGACTTCGTGGACAAGGCGGACCACCTCCGGCAGCAGACGTTGCGGAACATGCA
CAAGATCCTGACGCCGCGGCAGGCAGCGCGCGGCCTGCTCGCGCTCGCGGACTACGGTCAGCGCCTGCGCGCGCTCAGCT
CGCTCTGGGCGGCGCGCCCCAGGGAGCCCGCA
TFome clones do not contain stop codon at 3'end.
Translation: MGIYERQRHLVPTGVWGEPFRLDADAVAMPLPLAAFPSVTVATPAPLDVVEPEAEEIKLGKRLLQAQQDDVPRMQEAPPS
SDSFSHDDDDARPRDKVQRRLAQNREAARKSRLRKKAYIRNLETSRVKLAQLEQELIMARRQQHGAYGVGGGVAPPAAPV
DPRVAAFELEYAHWVEEQSRQATELRAALQSHAPDVQLRVLVDAALAHYGALFQAKARAARSDAFFVLSGVWRSPAERFF
LWIAGFRPSDLLKVLEPQLSPLMDHQASEVRKLQNTARQLEDALSQGMSKLQQTLVDTLMTVDVSPDGAGGGYAGQQMAC
AVGKLADLVDFVDKADHLRQQTLRNMHKILTPRQAARGLLALADYGQRLRALSSLWAARPREPA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map