TFome Information for pUT5458

TFome Name:pUT5458
Genbank Insert ID:KJ727598
Protein Name:ZmbZIP110
Species: Maize
TF Family: bZIP
Template: BT041487.1
Corresponding TF Gene: GRMZM2G079365
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C244_F 3' Primer Name: C244_R
5' Primer Sequence: caccATGGACCCGCGCTTCCC 3' Primer Sequence: CATGAAATCCATGGAGTCACTCAAGGG
5' Primer Tm (º C): 65.7 3' Primer Tm (º C): 59.2
PCR Condition: PCR Reaction Mix:5 l GC buffer (Thermo Fisher/ Finnzymes: contains 1.5 mM MgCl2) 1 l primer forward 20mM 1 l primer reverse 20mM 1.25 l DMSO 100% 1 l DNA template (c=1 ng/l) 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 14.5 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 55°C (DMSO conc. up to 10% decrease annealing temp. by 5.5-6.0°C) 40 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGACCCGCGCTTCCCGCCGCCGGCGCCGTCGTCCTGCTTCGGCGGGGGCGGGGAGCTCCCAGCACGGGGGCACCACCG
GCGGGCGCACTCGGAGACGTTCATCCGGCTGCCCGACGCAGACCTGCTGCTGGACCCGGACGCCGACTTCGGCTTCTCCG
ACATCGACTTCCCCTCTCTCTCCGACGACTCGCCCGCGGCGTCCGACCCGACGCCGCAGCCCCAGGCCCCGCCGCTCCAG
CCGCCGCTGCAGCAGGCGGCTTCCCCGGCGTCCGCGGCGCCGCCGAGGCCGCCGAGCGGGGCGCACATGCGGAGCCTCTC
CCTCGACGCCGCCTTCTTCGACGGCCTCTCGCTGCAGGGAGGCGGCGGCGGCGGGGGGGTAAGAGGGGGCATGGCGGGGC
ACAAGAGGAGCGGCTCCATGGACGGGGACACCTCGCCGTTCGAGGGCGAGTCGGCGCCGCCGTCCGTGCTGCCGGACTAC
GCCAAGAAGGCCGTGCCCGACGACAAGCTCGCCGAGCTCGCGCTCCTCGATCCCAAGCGCGCCAAGAGGATCCTGGCGAA
CCGGCAGTCCGCGGCGAGGTCCAAGGAGAGGAAGATCAAGTACACCAGTGAGCTGGAGAGGAAGGTGCAGACGCTGCAGA
CAGAGGCCACCACGCTTTCGGCACAGCTCACGCTTCTTCAGAGGGACACCTCTGGTTTAACCACTGAGAACAGGGAGCTG
AAACTCCGGTTGCAGGCCATGGAAGAACAAGCTAAACTTCGAGATGCTCTAAATGATGCCTTGAGGGAAGAAGTCCAGCG
GCTTAAGATAGCCGCAGGGCAGGTCCCCAACATGAACGGGAATCCCTTCAATGGAGGACTATCACAGCAGCAGCAGATGC
CAAGCTATTTCTCACAACCACAGCAGATGCAATACTTCAGCGGCCACCAGGGCCAGCATCACCATCCGAAGCATGACCCC
CAGAATTCTTCAAACGGCGGTGGCCAGCCCTTGAGTGACTCCATGGATTTCATG
TFome clones do not contain stop codon at 3'end.
Translation: MDPRFPPPAPSSCFGGGGELPARGHHRRAHSETFIRLPDADLLLDPDADFGFSDIDFPSLSDDSPAASDPTPQPQAPPLQ
PPLQQAASPASAAPPRPPSGAHMRSLSLDAAFFDGLSLQGGGGGGGVRGGMAGHKRSGSMDGDTSPFEGESAPPSVLPDY
AKKAVPDDKLAELALLDPKRAKRILANRQSAARSKERKIKYTSELERKVQTLQTEATTLSAQLTLLQRDTSGLTTENREL
KLRLQAMEEQAKLRDALNDALREEVQRLKIAAGQVPNMNGNPFNGGLSQQQQMPSYFSQPQQMQYFSGHQGQHHHPKHDP
QNSSNGGGQPLSDSMDFM
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map