TFome Information for pUT5459

TFome Name:pUT5459
Genbank Insert ID:KJ727599
Protein Name:ZmbZIP108
Species: Maize
TF Family: bZIP
Template: BT033992.1
Corresponding TF Gene: GRMZM2G033230
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: C249_F 3' Primer Name: C249_R
5' Primer Sequence: caccATGGACGATGGAGCTGACCTC 3' Primer Sequence: ATTTATCTTTAAAGCAAAGCCCAAAGAAGC
5' Primer Tm (º C): 62.9 3' Primer Tm (º C): 56.9
PCR Condition: PCR Reaction Mix:5 l GC buffer 1 l primer forward 20mM 1 l primer reverse 20mM 1.25 l DMSO 100% 1 l DNA template (c=1 ng/l) 1 l dNTPs 10mM 0.25 l phusion taq (FL-530) 14.5 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 60°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGACGATGGAGCTGACCTCCCCTGCCAGTTGCTCTTCTCCCACCCGGAGATGCCTCACAGCTTCGACGAGTTCTTCAG
CAGCGCCACGACCACGACCACCTGCACCCACACGCACAGCTGCGACCCTCCGGGCCCCTCGGCCGCCATGCATACCCACA
CGTGCCTGCACACGCACACCCAGGTCCTGGGCAGCGGCGGGGAGGACGACGGCTCAGCGAAGCCGCGGAGGCCCCTGGGA
AACAGGGAAGCCGTGCGCAAGTACCGCGAGAAGAAGAAGGCGCGCGCGGCCTTCCTGGAAGGGGAGGTCAAGAAGCTGCG
CGCCGCCAACCAGCAGCTCGTGCGGCGGCTGCAAGACCACGCGGCTCTGGAGGCGGAGGTGGCGAGGCTCAGGGGCCTCC
TGCTCGACGTCCGGGGCAGGATCGACGCCGAGGTCGGCGTGTCCCCGTTTCAGAGGCCATGCAGCGCGGGCTCTGTGGAG
TGCGGCGCTGATCCAGCTCTGCGCTTCGATGGCAGCTCGGAGGTAGTAGGAGGTGGTGGCTGGGAGAAGAGCTCAAGCCC
TCAAGTTGCGGACTGCAGGATCGATGAAGGGGGAGGAGGGATGTCACGGGAACTTGATGTTCTAGAAGTTTCGGGGCCTC
TGCCAGCTTCTTTGGGCTTTGCTTTAAAGATAAAT
TFome clones do not contain stop codon at 3'end.
Translation: MDDGADLPCQLLFSHPEMPHSFDEFFSSATTTTTCTHTHSCDPPGPSAAMHTHTCLHTHTQVLGSGGEDDGSAKPRRPLG
NREAVRKYREKKKARAAFLEGEVKKLRAANQQLVRRLQDHAALEAEVARLRGLLLDVRGRIDAEVGVSPFQRPCSAGSVE
CGADPALRFDGSSEVVGGGGWEKSSSPQVADCRIDEGGGGMSRELDVLEVSGPLPASLGFALKIN
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map