TFome Information for pUT5460

TFome Name:pUT5460
Genbank Insert ID:KJ727600
Protein Name:ZmbZIP7
Species: Maize
TF Family: bZIP
Template: BT039852.1
Corresponding TF Gene: GRMZM2G006578
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: C251_F 3' Primer Name: C251_R
5' Primer Sequence: caccATGGAGGAGTTGGCGGGG 3' Primer Sequence: TTGCTCCTGCGGTGCCCTG
5' Primer Tm (º C): 64.7 3' Primer Tm (º C): 63.5
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 1.25 l DMSO 100 50% 1 l DNA template (c=1 ng/l) 1 l dNTPs 10mM 0.25 l phusion taq (FL-530) 14.5 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 60°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGAGGAGTTGGCGGGGAGCAGGAGGCAGCAGGAGCACCTCCACCTGCAGCACCACCAACCCTTTGCTGCCGCTGCTGA
GCCAGCAGCACCCGGGATGATCAAAGATGTCAAGCCACTGGCCAAGAAGGATCACAGGAGAGGCACATCGACCGCTGAAC
GCGACCCAAAAACGCTGAGAAGGCTGGCCCAGAACAGGGAGGCTGCAAGGAAAAGCAGGCTAAGGAAGAAGGCTTACATC
CAGCAACTCGAGTCCAGCAGGATCAGACTAGCTCAGCTCGAACAAGAACTGCACACTGCAAGAGCTCAGGGAGTTTTCTT
CCCCAACAGTGGCATCCTCGCTGACCAAGGTGTCGCCGGCAAAGGCGTCCCCATCGGCGGCATCGACGGCCTCAGCTCAG
AGGCGGCGATGTTCGACGTGGAGTACGGGCGGTGGCAGGAGGAGCACTACCGGCTGATGTACGAGCTTCGGGCGGCGCTG
CAGCAGCACCTGCCGGAGGGGGAGCTGCAGATGTACGTGGAGAGCTGCCTGGCTCACCACGACGAGATGGTGGGCATCAA
AGAGGGCGCCATCAAGGGCGACGTCTTCCACCTCATCTCCGGCGTCTGGCGGAGCCCCGCCGAGCGCTGCTTCCTCTGGC
TCGGCGGCTTCCGCCCCTCCGAAGTCATCAAGATGCTGCTGAGCCACGTAGAGCCCCTGACCGAACAGCAGATCGTGGGC
GTGTACGGGCTGCAGCAGTCGGCGCTGGAGACGGAGGAAGCGCTGAGCCAGGGCCTGGAGGCGCTCTACCAGTCGCTGTC
TGACACCGTGGTCTCCGACGCGCTCAGCTGCCCCTCGAACGTCTCCAACTACATGGGTCAGATGGCCGTCGCCATGAACA
AGCTCTCCACGCTCGAGGGCTTCGTCAGACAAGCTGAGAACCTTCGGCAGCAGACGCTGCACCGGCTGCACCAGGTCCTG
ACGACGCGGCAGATGGCGCGGTCGCTGCTGGCCGTGTCGGACTACTTCCACCGCCTCCGCACGCTGAGCTCGCTCTGGGT
CACACGCCCCAGGGCACCGCAGGAGCAA
TFome clones do not contain stop codon at 3'end.
Translation: MEELAGSRRQQEHLHLQHHQPFAAAAEPAAPGMIKDVKPLAKKDHRRGTSTAERDPKTLRRLAQNREAARKSRLRKKAYI
QQLESSRIRLAQLEQELHTARAQGVFFPNSGILADQGVAGKGVPIGGIDGLSSEAAMFDVEYGRWQEEHYRLMYELRAAL
QQHLPEGELQMYVESCLAHHDEMVGIKEGAIKGDVFHLISGVWRSPAERCFLWLGGFRPSEVIKMLLSHVEPLTEQQIVG
VYGLQQSALETEEALSQGLEALYQSLSDTVVSDALSCPSNVSNYMGQMAVAMNKLSTLEGFVRQAENLRQQTLHRLHQVL
TTRQMARSLLAVSDYFHRLRTLSSLWVTRPRAPQEQ
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map