TFome Information for pUT5464

TFome Name:pUT5464
Genbank Insert ID:KJ727604
Protein Name:ZmbZIP70
Species: Maize
TF Family: bZIP
Template: BT055042.1
Corresponding TF Gene: GRMZM2G060290
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C284_F 3' Primer Name: C284_R
5' Primer Sequence: caccATGGCTTATCCATCCACCTCCG 3' Primer Sequence: CCCGGCCGGGCCTCTAGCC
5' Primer Tm (º C): 62.9 3' Primer Tm (º C): 68.6
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 40 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGCTTATCCATCCACCTCCGGGATGGTCCAAGCTTCTTCATCCCTCCATGGCAGCATGAGAAGGGATCCCGAAGGATA
TGACATGTCGTCGGATCTGGACCAAGCCCTACTCCTCTACTTCGATGGTCAGCAAGCCAAGACATCAGTTCAAGTTCAAG
AACAACAACCGCAGACTCTCAACATCTTCCCCTCCCAGCCAATGCACATCGGCCCATCCCCAAAGGGCTCCATGGCTTCT
TCTTCAGCTCCCGCAGCAGCAACACAGGTCGCTGGCCCTTCCAAGAACCCGCAGGCGCCGTCGTCGTTGAAGGTGGGAGG
CGGGCCGCCGCCGCTCGCCGCCGGTAAGAGCTCCAAGGCAGCAATCAAGAGAGAAGGTAGCGGCAGCGGCAAGCATCATG
GCGCCGCCGCGGGAGCTTCGAGCACGGCGGACCAGGAGGCCGGGCCCCGGACGCCGGACCCAAAGACCCTGCGGCGGCTC
GCGCAGAATAGGGAGGCCGCCAGGAAGAGCAGGTTGAGGAAGAAGGCTTATATCCAGCAGCTAGAGACAGGCAGGATCAG
GCTGGCACAGCTAGAGCAGGAGATGCAGATGGCAAGAACTCATCAGGGTGCACTATGGGGTGCAGGAACTCTAAGCCCAG
ACGCGGCGCTGTTCAACCTGGAGTACGAGCGGTGGCTGGGGGAGCACAGCAAGGTGGTGGCCCGTCTGCGGGCCGCCGCG
GAGGAGCACCGGCCGGACGTCGAGCTGCGGGCGTACGTGGACGAGGCCGCCGCGCACTACGGCGCGCTGATGGGCCACAA
GGCCCGGCTCGCCGCCGCCGACCCGCTCCACCTCCTCTCCGGCCTGTGGAAGGGCGCCGCCGAGCGCTGCTTCCTCTGGA
TCGGCGGCTTCCGCGCCTCCGACCTCGTCAAGGTCGTGGTCCGGCACGTGGAGCCGCTGGCGGAGCAGCAGGCGGCGGGG
GCGCGCGACGTGGAGCAGGCGGCGCGGCGGACAGAGGAGGCGCTCGACGCCGAGCTGGAGGCGCTGCTGCGGTCGCTCTC
CGAGGTCGTGTCCTCGGACGTGCAGCCGCCGGGGCCGGGGATGATGTACGGCGGCGGCGGCCAGCTCTACCACCCGGCCG
ACGTGGCCGGCTACATGGGGATGGGCCACATGCACGTCGCCCTCGCCATGGACAAGGTGGCCTCGCTCGGAACCATCCTC
AGACAGGCAGACGAGCTGAGGATGCAGGCGTTGCACGCGCTCCGGCAGATCCTGACGGCGAGGCAGGCGGCGCGCTGCTT
CGTCGCCGCCGATGACTACTTCTGCCGCCTCCGGACGCTCAGCGCGCTCTGGACCACCAGCCGGACGGCCCAGCTGGCTA
GAGGCCCGGCCGGG
TFome clones do not contain stop codon at 3'end.
Translation: MAYPSTSGMVQASSSLHGSMRRDPEGYDMSSDLDQALLLYFDGQQAKTSVQVQEQQPQTLNIFPSQPMHIGPSPKGSMAS
SSAPAAATQVAGPSKNPQAPSSLKVGGGPPPLAAGKSSKAAIKREGSGSGKHHGAAAGASSTADQEAGPRTPDPKTLRRL
AQNREAARKSRLRKKAYIQQLETGRIRLAQLEQEMQMARTHQGALWGAGTLSPDAALFNLEYERWLGEHSKVVARLRAAA
EEHRPDVELRAYVDEAAAHYGALMGHKARLAAADPLHLLSGLWKGAAERCFLWIGGFRASDLVKVVVRHVEPLAEQQAAG
ARDVEQAARRTEEALDAELEALLRSLSEVVSSDVQPPGPGMMYGGGGQLYHPADVAGYMGMGHMHVALAMDKVASLGTIL
RQADELRMQALHALRQILTARQAARCFVAADDYFCRLRTLSALWTTSRTAQLARGPAG
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map