TFome Information for pUT5465

TFome Name:pUT5465
Genbank Insert ID:KJ727605
Protein Name:ZmbZIP34
Species: Maize
TF Family: bZIP
Template: BT067808.1
Corresponding TF Gene: GRMZM2G045236
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C293_F 3' Primer Name: C293_R
5' Primer Sequence: caccATGGCGGAGCCGGCCATC 3' Primer Sequence: GTTCACAAGGTGTGGGCTGGCACTCTTG
5' Primer Tm (º C): 67.6 3' Primer Tm (º C): 64.9
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 40 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGCGGAGCCGGCCATCCTCGACCCCTCCCCCTTCGATCTCCGCCACTATCCGGCGCACCTCTTCGACCCGGACATCCC
CCTCGCCGCCGCCGACTTCCCACTCGGTGAATTCGCCGGAGACGACGGCTGTGATGGCCTAGACTTCGACGTGCCCGTAG
ATTTCTCTATCGACGACTTCCTCCTCCGGTCTCCTGACCGCGGGCACGACGGCGACGACTCCGGCGAGGGCTCTGCTGCC
GGATCCGGCCCCACCGCATCTTCCTTGGCCTCCCCGGCCACCTCCGGCACTAACTCCGCCGTGGCCAACGTCGGCAACCG
CAAGGTGAAGCACGAGGACTCGGACGAGGGAAGGAGCGGCGCCACCCCGAATTGGAGCCTCAAGCGGAAGCAGGTGAGTC
CCGGAGAAGCAACCTCGGATGGGGCCAAGTGCCGCCGATCCGGCGATGGGGATGTTTCGCCATCTGCATCGGCGTCGCGT
GCAGCCACGGAGGACTCCGATGAGAGGGGCGTGGGCGGCGAGGAAGAGGACAAGCGCCGGACTGCGCGCCTGATGCGGAA
CCGTGAGAGCGCGCAGCTCTCTCGGCAGAGGAAGAAGCGGTACGTGGAGGAGCTGGAAGAGAAGGTGAAATCGATGCACT
CGGTGATAAATGACCTCAGTTGTAAGATCTCCTTCATCGCTGCGGAGAACGCCACACTGAGGCAGCAACTCGGTGGTGTG
GGGGTGAGTGGACCACCACCTGGGGTGTATCCACCGCCACCGCCAGGCATTCATTTCCCTTGGGTTTCTGGGTATGCAAT
GCGGCCTCATGGCTCCCATGTTCCACTTGTTCCAATCCCGCGATTGAAGCCGCAGCATGCAGCAGCTGCAGTGAAGGTAT
CCAAGAAACCAGATGTCAAGAAGTCTGTGGAGAGCAAGAGTAAGACCAAGACCAAGACCAAGACCAAGAAGGTGGCAAGT
ATTAGCCTTCTTGGTTTGCTGTTTGTTGCATTTGTCTTTGGCGCTTTTGTTCCGGGGTTCAATCATAGTCTCGGACTGAG
AGGAAGGAGCGACAATGTGATGTTTGGAAATTTTGGCCAGTCTGATGCTAGGGTGTTTAGTGTCACTAACCATGGTAAAG
GACCTAAAGGCAGTTTCAACAGTAGTGACATGATCAGTACTGATCATGACATGATGACTGGGCATGATGATGGAGCGGGG
CAGAAACACCGAGCTGCAAATAACTCTAGTGAGATCTTGCCTGCTTTGTTGTATGTGCCGAGGAATGGAAAACATGTTAT
GATCAATGGCAATTTGATTATTCATTCTGTGATGGCTAGTGAGAAAGCAGTGGCACATAAGGGCTCAAATAGTCAGTCAG
TTAAAGATCACAAGGAGACCAGTGTTGCTATAGCTCGCTATCTGTCACCACCTGGAAAGGATATGAATTCAAAAGAGATG
TTCCCGCCAGATGCACCACTGCCACAATGGTTCCGTGAAGGAATGGAAGGACCTGTTTTGAACTCGGGAATGTGCAGTGA
GGTATTTCAGTTTGATATTTCTCCAGCTTCGGCCAAATCTGGTGGCATTATACCTGCTTCGGCCAAATCTGCCTCAAGTG
TCAATGCTACCCAGAAAATTCTGAAACCTTCTCCTGCTTATAGGGGCAAGCTGAAAAACAGGAGGATCATGTATAACGAG
GCAATTCCACTTACTGGAAAAACAGCGAACAACACAAAGCCTCAGGCTTTTAACAGTACTGCTGAAAGCAGCAAGGTACC
TGATACTGATAGCAAGCCTGCATCATCAGTTATCGTCTCTGTGCTAGCTGATCCCAGGGAGGCTGGCAATGGGGATGGTG
ATCCAAGAGTATCACCAAAACCCCTCTCCAGGATATTTGTCGTCGTTCTGCTCGATGGTGTCAGATACGTGACTTACTCG
TGCACACTTCCTTTCAAGAGTGCCAGCCCACACCTTGTGAAC
TFome clones do not contain stop codon at 3'end.
Translation: MAEPAILDPSPFDLRHYPAHLFDPDIPLAAADFPLGEFAGDDGCDGLDFDVPVDFSIDDFLLRSPDRGHDGDDSGEGSAA
GSGPTASSLASPATSGTNSAVANVGNRKVKHEDSDEGRSGATPNWSLKRKQVSPGEATSDGAKCRRSGDGDVSPSASASR
AATEDSDERGVGGEEEDKRRTARLMRNRESAQLSRQRKKRYVEELEEKVKSMHSVINDLSCKISFIAAENATLRQQLGGV
GVSGPPPGVYPPPPPGIHFPWVSGYAMRPHGSHVPLVPIPRLKPQHAAAAVKVSKKPDVKKSVESKSKTKTKTKTKKVAS
ISLLGLLFVAFVFGAFVPGFNHSLGLRGRSDNVMFGNFGQSDARVFSVTNHGKGPKGSFNSSDMISTDHDMMTGHDDGAG
QKHRAANNSSEILPALLYVPRNGKHVMINGNLIIHSVMASEKAVAHKGSNSQSVKDHKETSVAIARYLSPPGKDMNSKEM
FPPDAPLPQWFREGMEGPVLNSGMCSEVFQFDISPASAKSGGIIPASAKSASSVNATQKILKPSPAYRGKLKNRRIMYNE
AIPLTGKTANNTKPQAFNSTAESSKVPDTDSKPASSVIVSVLADPREAGNGDGDPRVSPKPLSRIFVVVLLDGVRYVTYS
CTLPFKSASPHLVN
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map